Construct: ORF ccsbBroad304_07839

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF010757.1_s304c1, BRDN0000403840
Derived from:
ccsbBroadEn_07839
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
SPART (23111)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_07839
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 23111 SPART spartin NM_001142294.1 99.8% 99.6% 205C>A;1250C>A;1629A>G
2 human 23111 SPART spartin NM_001142295.1 99.8% 99.6% 205C>A;1250C>A;1629A>G
3 human 23111 SPART spartin NM_001142296.2 99.8% 99.6% 205C>A;1250C>A;1629A>G
4 human 23111 SPART spartin NM_015087.5 99.8% 99.6% 205C>A;1250C>A;1629A>G
5 human 23111 SPART spartin XM_005266313.5 99.8% 99.6% 205C>A;1250C>A;1629A>G
6 human 23111 SPART spartin XM_005266314.3 99.8% 99.6% 205C>A;1250C>A;1629A>G
7 human 23111 SPART spartin XM_005266315.3 99.8% 99.6% 205C>A;1250C>A;1629A>G
8 human 23111 SPART spartin XM_005266317.3 99.8% 99.6% 205C>A;1250C>A;1629A>G
9 human 23111 SPART spartin XM_011535012.2 99.8% 99.6% 205C>A;1250C>A;1629A>G
10 human 23111 SPART spartin XM_024449334.1 99.8% 99.6% 205C>A;1250C>A;1629A>G
11 human 23111 SPART spartin XR_001749523.2 39.3% (many diffs)
12 mouse 229285 Spg20 spastic paraplegia 20, spar... NM_001144987.1 80.6% 84.8% (many diffs)
13 mouse 229285 Spg20 spastic paraplegia 20, spar... NM_144895.2 80.6% 84.8% (many diffs)
14 mouse 229285 Spg20 spastic paraplegia 20, spar... XM_006501320.2 80.6% 84.8% (many diffs)
15 mouse 229285 Spg20 spastic paraplegia 20, spar... NM_001144988.1 75.3% 80.2% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
2064
ORF length:
1998
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgga gcaagagcca caaaatggag aacctgctga aattaagatc atcagagaag
121catataagaa ggccttttta tttgttaaca aaggtctgaa tacagatgaa ttaggtcaga
181aggaagaagc aaagaactac tataagcaag gaataggaca cctgctcaga gggatcagca
241tttcatcaaa agagtctgaa cacacaggta ctgggtggga atctgctaga cagatgcaac
301agaaaatgaa agaaactcta cagaatgtac gcaccaggct ggaaattcta gagaagggtc
361ttgccacttc tctgcagaat gatcttcagg aggtgcccaa gttatatcca gaatttccac
421ctaaagacat gtgtgaaaaa ttaccagagc ctcagtcttt tagttcagct cctcagcatg
481ctgaagtaaa tggaaacacc tcaactccaa gtgcaggggc agttgctgca cctgcttctc
541tgtctttacc atcacaaagt tgtccagcag aagctcctcc tgcttatact cctcaagctg
601ctgaaggtca ctacactgta tcctatggaa cagattctgg ggagttttca tcagttggag
661aggagtttta taggaatcat tctcagccac cgcctcttga gaccttaggg ctggatgcag
721atgaattgat tttgatacca aatggagtac agattttttt tgtaaatcct gcaggggagg
781ttagtgcacc ttcgtatcct gggtaccttc gaattgtgag gtttttggat aattctctcg
841atacggttct aaaccgtcct cccgggtttc ttcaggtttg tgactggtta tatcctctag
901ttcctgatag atctccggtt ctgaaatgta ctgcgggagc ctacatgttt cctgatacaa
961tgctacaagc agcaggatgc tttgtggggg tcgtcctgtc ctctgagtta ccagaggatg
1021atagagagct ctttgaggat ctgttaaggc aaatgtctga ccttcggctc caggccaact
1081ggaacagagc agaagaagaa aatgaattcc aaatccctgg aagaactaga ccctcctctg
1141accaactaaa agaagcctct ggcactgatg tgaaacagtt ggaccaaggc aataaggatg
1201tacgtcataa aggaaaacgt ggaaaaaggg ctaaagatac ttcaagtgaa gaagttaacc
1261tgagtcacat tgtaccatgt gagccagttc cagaagaaaa gccaaaagaa ttacatgaat
1321ggagtgaaaa agtggctcac aacattttgt caggtgcttc ctgggtgagt tggggtttag
1381tcaaaggtgc tgagattact ggtaaggcaa tccagaaagg tgcttctaaa ctccgagagc
1441ggattcaacc agaagaaaaa cccgtggaag ttagtccagc tgtcaccaag ggactttata
1501tagcgaagca agctacagga ggagcagcaa aagtcagtca gttcctggtt gatggagttt
1561gcactgtagc aaattgcgtt ggaaaagaac tagctccaca tgtcaagaag catggaagca
1621aacttgttcc agaatctctt aaaaaagaca aagatgggaa atctcctctg gatggtgcta
1681tggttgtagc agcgagtagt gttcaaggat tttcaactgt ctggcaagga ttggaatgtg
1741cagctaaatg catcgttaac aatgtttcag cagaaactgt acaaactgtc agatacaaat
1801acggatataa tgcaggagaa gctacccacc atgcggtgga ttctgcggtc aatgttggcg
1861taactgccta caatattaac aacattggta tcaaagcaat ggtgaagaaa actgcaacac
1921aaacaggaca cactctcctt gaggactatc agatagttga taattctcag agggaaaatc
1981aagaaggagc agcaaatgtc aacgtgagag gggagaagga tgagcagacg aaggaagtaa
2041aggaggcaaa gaagaaagat aaatgcccaa ctttcttgta caaagtggtt ggtaagccta
2101tccctaaccc tctcctcggt ctcgattcta cgtagtaatg agctagcgct aaccggtggc
2161gcgttaagtc gacaatcaac ctctggatta

Download FASTA (ORF) (Full)