Transcript: Mouse NM_001144987.1

Mus musculus spastic paraplegia 20, spartin (Troyer syndrome) homolog (human) (Spg20), transcript variant 2, mRNA.

Source:
NCBI, updated 2017-05-21
Taxon:
Mus musculus (mouse)
Gene:
Spg20 (229285)
Length:
4013
CDS:
329..2344

Additional Resources:

NCBI RefSeq record:
NM_001144987.1
NBCI Gene record:
Spg20 (229285)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_001144987.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000249048 TCGTGCGGTTCTTGGATAATT pLKO_005 1077 CDS 100% 15.000 21.000 N Spg20 n/a
2 TRCN0000249047 GACTGAACACGGACGAGTTAG pLKO_005 417 CDS 100% 10.800 15.120 N Spg20 n/a
3 TRCN0000183279 GCGAATTGTGTTGGTAAAGAA pLKO.1 1844 CDS 100% 5.625 7.875 N Spg20 n/a
4 TRCN0000249044 CAGGTGTGTGACTGGTTATAC pLKO_005 1136 CDS 100% 13.200 9.240 N Spg20 n/a
5 TRCN0000179319 GCGTCTTCTGTCTGTTCATTT pLKO.1 2422 3UTR 100% 13.200 9.240 N Spg20 n/a
6 TRCN0000249046 GCGTCTTCTGTCTGTTCATTT pLKO_005 2422 3UTR 100% 13.200 9.240 N Spg20 n/a
7 TRCN0000249045 TTGGGCTCACTGCCTACAATA pLKO_005 2130 CDS 100% 13.200 9.240 N Spg20 n/a
8 TRCN0000196037 GAACAGCGTCTTCTGTCTGTT pLKO.1 2417 3UTR 100% 0.495 0.347 N Spg20 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001144987.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_07839 pDONR223 100% 80.6% 84.8% None (many diffs) n/a
2 ccsbBroad304_07839 pLX_304 0% 80.6% 84.8% V5 (many diffs) n/a
3 TRCN0000478689 GTTCCGTTCACCGACTGAGGGTAG pLX_317 9.7% 80.6% 84.8% V5 (many diffs) n/a
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