Construct: ORF ccsbBroad304_07842
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000277.1_s304c1, BRDN0000402005
- Derived from:
- ccsbBroadEn_07842
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FCHO1 (23149)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_07842
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 23149 | FCHO1 | FCH and mu domain containin... | NM_001161357.2 | 99.8% | 100% | 903A>G;1152G>A;1899A>G |
| 2 | human | 23149 | FCHO1 | FCH and mu domain containin... | NM_001161358.2 | 99% | 99.1% | (many diffs) |
| 3 | human | 23149 | FCHO1 | FCH and mu domain containin... | NM_015122.3 | 99% | 99.1% | (many diffs) |
| 4 | human | 23149 | FCHO1 | FCH and mu domain containin... | XM_006722701.4 | 99% | 99.1% | (many diffs) |
| 5 | human | 23149 | FCHO1 | FCH and mu domain containin... | XM_006722702.4 | 99% | 99.1% | (many diffs) |
| 6 | human | 23149 | FCHO1 | FCH and mu domain containin... | XM_011527824.2 | 99% | 99.1% | (many diffs) |
| 7 | human | 23149 | FCHO1 | FCH and mu domain containin... | XM_011527825.2 | 99% | 99.1% | (many diffs) |
| 8 | human | 23149 | FCHO1 | FCH and mu domain containin... | XM_011527826.3 | 99% | 99.1% | (many diffs) |
| 9 | human | 23149 | FCHO1 | FCH and mu domain containin... | XM_011527828.2 | 99% | 99.1% | (many diffs) |
| 10 | human | 23149 | FCHO1 | FCH and mu domain containin... | XM_017026519.1 | 99% | 99.1% | (many diffs) |
| 11 | human | 23149 | FCHO1 | FCH and mu domain containin... | XM_017026520.2 | 99% | 99.1% | (many diffs) |
| 12 | human | 23149 | FCHO1 | FCH and mu domain containin... | XM_017026521.2 | 99% | 99.1% | (many diffs) |
| 13 | human | 23149 | FCHO1 | FCH and mu domain containin... | XM_024451427.1 | 99% | 99.1% | (many diffs) |
| 14 | human | 23149 | FCHO1 | FCH and mu domain containin... | XM_024451428.1 | 99% | 99.1% | (many diffs) |
| 15 | human | 23149 | FCHO1 | FCH and mu domain containin... | XM_017026522.1 | 94.7% | 94.7% | (many diffs) |
| 16 | human | 23149 | FCHO1 | FCH and mu domain containin... | XM_017026523.1 | 94.7% | 94.7% | (many diffs) |
| 17 | human | 23149 | FCHO1 | FCH and mu domain containin... | XM_017026524.2 | 94.7% | 94.7% | (many diffs) |
| 18 | human | 23149 | FCHO1 | FCH and mu domain containin... | XM_017026525.1 | 94.7% | 94.7% | (many diffs) |
| 19 | human | 23149 | FCHO1 | FCH and mu domain containin... | NM_001161359.2 | 93.4% | 93.4% | (many diffs) |
| 20 | human | 23149 | FCHO1 | FCH and mu domain containin... | XM_011527827.2 | 88.7% | 88.7% | (many diffs) |
| 21 | human | 23149 | FCHO1 | FCH and mu domain containin... | XM_017026526.1 | 88.7% | 88.7% | (many diffs) |
| 22 | human | 23149 | FCHO1 | FCH and mu domain containin... | XM_017026527.2 | 88.7% | 88.7% | (many diffs) |
| 23 | human | 23149 | FCHO1 | FCH and mu domain containin... | XM_017026528.1 | 88.7% | 88.7% | (many diffs) |
| 24 | human | 23149 | FCHO1 | FCH and mu domain containin... | XR_002958285.1 | 75.5% | (many diffs) | |
| 25 | human | 23149 | FCHO1 | FCH and mu domain containin... | XM_005259831.4 | 74.6% | 68.7% | (many diffs) |
| 26 | human | 23149 | FCHO1 | FCH and mu domain containin... | XM_024451429.1 | 74.6% | 68.7% | (many diffs) |
| 27 | human | 23149 | FCHO1 | FCH and mu domain containin... | XM_024451430.1 | 74.6% | 68.7% | (many diffs) |
| 28 | human | 23149 | FCHO1 | FCH and mu domain containin... | XM_024451431.1 | 45.5% | 44.2% | (many diffs) |
| 29 | mouse | 74015 | Fcho1 | FCH domain only 1 | NM_028715.3 | 80.6% | 85.3% | (many diffs) |
| 30 | mouse | 74015 | Fcho1 | FCH domain only 1 | XM_006509765.2 | 80.6% | 85.3% | (many diffs) |
| 31 | mouse | 74015 | Fcho1 | FCH domain only 1 | XM_006509766.3 | 80.6% | 85.3% | (many diffs) |
| 32 | mouse | 74015 | Fcho1 | FCH domain only 1 | XM_006509767.3 | 80.6% | 85.3% | (many diffs) |
| 33 | mouse | 74015 | Fcho1 | FCH domain only 1 | XM_011242331.2 | 75.6% | 80% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2742
- ORF length:
- 2673
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat gtcgtatttt ggggagcatt tttggggcga gaaaaatcat ggctttgagg 121 tcctgtacca cagcgtgaag caggggccca tctccaccaa ggagctggcg gacttcatcc 181 gggagagggc caccatcgag gagacctact cgaaggcgat ggcgaaactc tccaagctgg 241 ccagcaacgg gacccccatg gggaccttcg ccccgctctg ggaggtcttc cgcgtctcct 301 cggacaagct ggcgctgtgc cacctggaac tgacacggaa gttacaggat ctcatcaagg 361 acgttctccg ctacggcgag gaacagctca agacccacaa gaagtgcaag gaggaagtgg 421 tgagcacctt ggatgctgtg caggtactct cgggcgtcag ccagctcctg cccaagtccc 481 gcgagaacta cctgaaccgt tgcatggacc aggagcggct gcggagggag agtaccagcc 541 agaaggagat ggacaaggcg gagactaaaa ccaagaaggc ggcagagagc ctgcggcgct 601 cagtggaaaa atacaactca gcccgagctg actttgagca gaagatgctg gactcagccc 661 tgcgcttcca agccatggag gagacacacc tgaggcacat gaaggcactg ctgggctcat 721 atgctcactc ggtggaggac acgcacgtgc agattgggca ggtgcatgag gaatttaagc 781 agaacatcga gaacgtcagc gtggagatgc tactcaggaa gtttgcagag agtaagggca 841 caggccggga gaagcctgga cctctggact tcgaggcata cagtgcggct gccctgcagg 901 aagcgatgaa acgtttgcgg ggagccaagg cctttcgcct tccaggacta agccggcggg 961 agcgggagcc ggagccacct gcagctgtag atttcctgga gcccgattca gggacatgtc 1021 cagaggtgga tgaagaaggt ttcactgtcc ggcctgatgt gacccagaac agcacggccg 1081 agccctcccg tttctcgtcc agcgactccg acttcgacga tgaagagccc cgcaagttct 1141 atgtgcacat caagcctgcc ccggcccggg ctccagcctg cagccccgag gcagcagcgg 1201 cacagctcag ggccaccgca ggcagcctca tccttcctcc tggcccaggg ggcaccatga 1261 aacgccattc ttcacgggac gctgctggga aaccccagag acctcggtct gcccccagaa 1321 ccagcagctg tgcagagaga ttgcagtcag aggagcaggt gtccaagaac ctctttgggc 1381 cgcccctgga gtcagccttt gaccacgaag attttacagg ctctagcagc ctgggcttca 1441 cctccagccc ctcccctttc tcctcctcgt cgcccgaaaa cgtggaggat tccggcctgg 1501 actctccgtc ccacgcggca cctggcccct ccccagattc ctgggtcccc cgcccaggca 1561 ccccgcagag cccgcccagc tgtagggcgc cacccccaga ggccaggggt atccgggcac 1621 cgcctctgcc agactcgccg cagcccctcg cctcgtctcc aggcccctgg gggctggagg 1681 ccttggccgg aggagacctg atgcctgcac ctgctgaccc cacagccagg gagggcctgg 1741 cagccccacc caggagactt cgctctagga aggtgtcctg ccctctcaca cgtagcaatg 1801 gggacctgtc tcgttccctg agcccctccc cactgggctc ttcagccgcc agcactgcct 1861 tggaacggcc cagcttctta tcccagacag gacacggagt ctcccggggt ccgagccctg 1921 tggtcctggg ctcccaggat gccctgccca tagccacagc cttcacggag tatgtccacg 1981 cctacttccg tggccacagc cccagctgcc tggctcgagt aactggggag ctgaccatga 2041 ccttccctgc tggcatcgtg cgtgtgttca gcgggacccc accaccacct gtcctcagct 2101 tccggcttgt acacacaacc gctattgagc acttccagcc caacgccgat ctgctgttca 2161 gtgacccctc ccagagtgac cctgagacca aagacttctg gctcaacatg gcagctctga 2221 ccgaagccct gcagcgccag gcagagcaga accccactgc ctcctactac aacgtggtgc 2281 tgctgcgata ccagttctcc cgcccgggtc cccagtctgt gcctctgcag ctcagtgccc 2341 actggcagtg tggagccacc ctcacccagg tctcagtgga gtacggctac cggcccggtg 2401 ccacggctgt gcccacacca ctcacgaacg tccagatcct gctgcctgtg ggggagcctg 2461 tgaccaacgt ccgcttgcag ccggctgcca cctggaacct ggaggagaag cggctcactt 2521 ggaggcttcc agatgtgtcc gaggcaggcg gttctggccg cctctctgcc agctgggagc 2581 cgctctcagg gcccagcaca cccagccccg tggctgcaca gttcaccagc gaggggacca 2641 ctctgtcggg cgtggacttg gaactggtgg gcagcggtta ccgcatgtcg ctggtgaaga 2701 ggaggtttgc cacagctgca cccccacagg gatgtacctg gttgccaact ttcttgtaca 2761 aagtggttgg taagcctatc cctaaccctc tcctcggtct cgattctacg tagtaatgag 2821 ctagcgctaa ccggtggcgc gttaagtcga caatcaacct ctggatta