Transcript: Human NM_001161357.2

Homo sapiens FCH and mu domain containing endocytic adaptor 1 (FCHO1), transcript variant 1, mRNA.

Source:
NCBI, updated 2019-07-21
Taxon:
Homo sapiens (human)
Gene:
FCHO1 (23149)
Length:
3192
CDS:
254..2929

Additional Resources:

NCBI RefSeq record:
NM_001161357.2
NBCI Gene record:
FCHO1 (23149)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001161357.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000164648 GCAGGAAGCGATGAAACGTTT pLKO.1 1081 CDS 100% 4.950 6.930 N FCHO1 n/a
2 TRCN0000162794 CACAACCGCTATTGAGCACTT pLKO.1 2299 CDS 100% 4.050 5.670 N FCHO1 n/a
3 TRCN0000164546 CCGCAAGTTCTATGTGCACAT pLKO.1 1315 CDS 100% 4.050 3.240 N FCHO1 n/a
4 TRCN0000162472 CAAAGACTTCTGGCTCAACAT pLKO.1 2374 CDS 100% 4.950 3.465 N FCHO1 n/a
5 TRCN0000159485 CAAGTTCTATGTGCACATCAA pLKO.1 1318 CDS 100% 4.950 3.465 N FCHO1 n/a
6 TRCN0000161066 GCAAGTTCTATGTGCACATCA pLKO.1 1317 CDS 100% 4.950 3.465 N FCHO1 n/a
7 TRCN0000163801 GCTTGTACACACAACCGCTAT pLKO.1 2290 CDS 100% 4.050 2.835 N FCHO1 n/a
8 TRCN0000161469 GCAGGTGCATGAGGAATTTAA pLKO.1 943 CDS 100% 15.000 9.000 N FCHO1 n/a
9 TRCN0000161312 GAGATGCTACTCAGGAAGTTT pLKO.1 989 CDS 100% 5.625 3.375 N FCHO1 n/a
10 TRCN0000164206 CAGAGGTGGATGAAGAAGGTT pLKO.1 1206 CDS 100% 3.000 1.800 N FCHO1 n/a
11 TRCN0000163060 CAAGACCCACAAGAAGTGCAA pLKO.1 574 CDS 100% 2.640 1.584 N FCHO1 n/a
12 TRCN0000097753 CATGAGGAATTTAAGCAGAAT pLKO.1 950 CDS 100% 4.950 3.465 N Fcho1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001161357.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_07842 pDONR223 100% 99.8% 100% None 903A>G;1152G>A;1899A>G n/a
2 ccsbBroad304_07842 pLX_304 0% 99.8% 100% V5 903A>G;1152G>A;1899A>G n/a
3 ccsbBroadEn_07843 pDONR223 100% 99% 99.1% None (many diffs) n/a
4 ccsbBroad304_07843 pLX_304 0% 99% 99.1% V5 (many diffs) n/a
5 TRCN0000479950 CCATGGCACCTAACTAAGATTGTA pLX_317 14.1% 99% 99.1% V5 (many diffs) n/a
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