Construct: ORF ccsbBroad304_07843
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014350.1_s304c1, BRDN0000461745
- Derived from:
- ccsbBroadEn_07843
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FCHO1 (23149)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_07843
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 23149 | FCHO1 | FCH and mu domain containin... | NM_001161358.2 | 99.8% | 100% | 903A>G;1152G>A;1899A>G |
| 2 | human | 23149 | FCHO1 | FCH and mu domain containin... | NM_015122.3 | 99.8% | 100% | 903A>G;1152G>A;1899A>G |
| 3 | human | 23149 | FCHO1 | FCH and mu domain containin... | XM_006722701.4 | 99.8% | 100% | 903A>G;1152G>A;1899A>G |
| 4 | human | 23149 | FCHO1 | FCH and mu domain containin... | XM_006722702.4 | 99.8% | 100% | 903A>G;1152G>A;1899A>G |
| 5 | human | 23149 | FCHO1 | FCH and mu domain containin... | XM_011527824.2 | 99.8% | 100% | 903A>G;1152G>A;1899A>G |
| 6 | human | 23149 | FCHO1 | FCH and mu domain containin... | XM_011527825.2 | 99.8% | 100% | 903A>G;1152G>A;1899A>G |
| 7 | human | 23149 | FCHO1 | FCH and mu domain containin... | XM_011527826.3 | 99.8% | 100% | 903A>G;1152G>A;1899A>G |
| 8 | human | 23149 | FCHO1 | FCH and mu domain containin... | XM_011527828.2 | 99.8% | 100% | 903A>G;1152G>A;1899A>G |
| 9 | human | 23149 | FCHO1 | FCH and mu domain containin... | XM_017026519.1 | 99.8% | 100% | 903A>G;1152G>A;1899A>G |
| 10 | human | 23149 | FCHO1 | FCH and mu domain containin... | XM_017026520.2 | 99.8% | 100% | 903A>G;1152G>A;1899A>G |
| 11 | human | 23149 | FCHO1 | FCH and mu domain containin... | XM_017026521.2 | 99.8% | 100% | 903A>G;1152G>A;1899A>G |
| 12 | human | 23149 | FCHO1 | FCH and mu domain containin... | XM_024451427.1 | 99.8% | 100% | 903A>G;1152G>A;1899A>G |
| 13 | human | 23149 | FCHO1 | FCH and mu domain containin... | XM_024451428.1 | 99.8% | 100% | 903A>G;1152G>A;1899A>G |
| 14 | human | 23149 | FCHO1 | FCH and mu domain containin... | NM_001161357.2 | 99% | 99.1% | (many diffs) |
| 15 | human | 23149 | FCHO1 | FCH and mu domain containin... | XM_017026522.1 | 95.5% | 95.6% | (many diffs) |
| 16 | human | 23149 | FCHO1 | FCH and mu domain containin... | XM_017026523.1 | 95.5% | 95.6% | (many diffs) |
| 17 | human | 23149 | FCHO1 | FCH and mu domain containin... | XM_017026524.2 | 95.5% | 95.6% | (many diffs) |
| 18 | human | 23149 | FCHO1 | FCH and mu domain containin... | XM_017026525.1 | 95.5% | 95.6% | (many diffs) |
| 19 | human | 23149 | FCHO1 | FCH and mu domain containin... | NM_001161359.2 | 94.2% | 94.3% | (many diffs) |
| 20 | human | 23149 | FCHO1 | FCH and mu domain containin... | XM_011527827.2 | 89.5% | 89.6% | (many diffs) |
| 21 | human | 23149 | FCHO1 | FCH and mu domain containin... | XM_017026526.1 | 89.5% | 89.6% | (many diffs) |
| 22 | human | 23149 | FCHO1 | FCH and mu domain containin... | XM_017026527.2 | 89.5% | 89.6% | (many diffs) |
| 23 | human | 23149 | FCHO1 | FCH and mu domain containin... | XM_017026528.1 | 89.5% | 89.6% | (many diffs) |
| 24 | human | 23149 | FCHO1 | FCH and mu domain containin... | XR_002958285.1 | 76.1% | (many diffs) | |
| 25 | human | 23149 | FCHO1 | FCH and mu domain containin... | XM_005259831.4 | 75.3% | 69.5% | (many diffs) |
| 26 | human | 23149 | FCHO1 | FCH and mu domain containin... | XM_024451429.1 | 75.3% | 69.5% | (many diffs) |
| 27 | human | 23149 | FCHO1 | FCH and mu domain containin... | XM_024451430.1 | 75.3% | 69.5% | (many diffs) |
| 28 | human | 23149 | FCHO1 | FCH and mu domain containin... | XM_024451431.1 | 45.6% | 44.3% | (many diffs) |
| 29 | mouse | 74015 | Fcho1 | FCH domain only 1 | NM_028715.3 | 81.3% | 86.1% | (many diffs) |
| 30 | mouse | 74015 | Fcho1 | FCH domain only 1 | XM_006509765.2 | 81.3% | 86.1% | (many diffs) |
| 31 | mouse | 74015 | Fcho1 | FCH domain only 1 | XM_006509766.3 | 81.3% | 86.1% | (many diffs) |
| 32 | mouse | 74015 | Fcho1 | FCH domain only 1 | XM_006509767.3 | 81.3% | 86.1% | (many diffs) |
| 33 | mouse | 74015 | Fcho1 | FCH domain only 1 | XM_011242331.2 | 76.3% | 80.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2733
- ORF length:
- 2667
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgtc gtattttggg gagcattttt ggggcgagaa aaatcatggc tttgaggtcc 121 tgtaccacag cgtgaagcag gggcccatct ccaccaagga gctggcggac ttcatccggg 181 agagggccac catcgaggag acctactcga aggcgatggc gaaactctcc aagctggcca 241 gcaacgggac ccccatgggg accttcgccc cgctctggga ggtcttccgc gtctcctcgg 301 acaagctggc gctgtgccac ctggaactga cacggaagtt acaggatctc atcaaggacg 361 ttctccgcta cggcgaggaa cagctcaaga cccacaagaa gtgcaaggag gaagtggtga 421 gcaccttgga tgctgtgcag gtactctcgg gcgtcagcca gctcctgccc aagtcccgcg 481 agaactacct gaaccgttgc atggaccagg agcggctgcg gagggagagt accagccaga 541 aggagatgga caaggcggag actaaaacca agaaggcggc agagagcctg cggcgctcag 601 tggaaaaata caactcagcc cgagctgact ttgagcagaa gatgctggac tcagccctgc 661 gcttccaagc catggaggag acacacctga ggcacatgaa ggcactgctg ggctcatatg 721 ctcactcggt ggaggacacg cacgtgcaga ttgggcaggt gcatgaggaa tttaagcaga 781 acatcgagaa cgtcagcgtg gagatgctac tcaggaagtt tgcagagagt aagggcacag 841 gccgggagaa gcctggacct ctggacttcg aggcatacag tgcggctgcc ctgcaggaag 901 cgatgaaacg tttgcgggga gccaaggcct ttcgccttcc aggactaagc cggcgggagc 961 gggagccgga gccacctgca gctgtagatt tcctggagcc cgattcaggg acatgtccag 1021 aggtggatga agaaggtttc actgtccggc ctgatgtgac ccagaacagc acggccgagc 1081 cctcccgttt ctcgtccagc gactccgact tcgacgatga agagccccgc aagttctatg 1141 tgcacatcaa gcctgccccg gcccgggctc cagcctgcag ccccgaggca gcagcggcac 1201 agctcagggc caccgcaggc agcctcatcc ttcctcctgg cccagggggc accatgaaac 1261 gccattcttc acgggacgct gctgggaaac cccagagacc tcggtctgcc cccagaacca 1321 gcagctgtgc agagagattg cagtcagagg agcaggtgtc caagaacctc tttgggccgc 1381 ccctggagtc agcctttgac cacgaagatt ttacaggctc tagcagcctg ggcttcacct 1441 ccagcccctc ccctttctcc tcctcgtcgc ccgaaaacgt ggaggattcc ggcctggact 1501 ctccgtccca cgcggcacct ggcccctccc cagattcctg ggtcccccgc ccaggcaccc 1561 cgcagagccc gcccagctgt agggcgccac ccccagaggc caggggtatc cgggcaccgc 1621 ctctgccaga ctcgccgcag cccctcgcct cgtctccagg cccctggggg ctggaggcct 1681 tggccggagg agacctgatg cctgcacctg ctgaccccac agccagggag ggcctggcag 1741 ccccacccag gagacttcgc tctaggaagg tgtcctgccc tctcacacgt agcaatgggg 1801 acctgtctcg ttccctgagc ccctccccac tgggctcttc agccgccagc actgccttgg 1861 aacggcccag cttcttatcc cagacaggac acggagtctc ccggggtccg agccctgtgg 1921 tcctgggctc ccaggatgcc ctgcccatag ccacagcctt cacggagtat gtccacgcct 1981 acttccgtgg ccacagcccc agctgcctgg ctcgagtaac tggggagctg accatgacct 2041 tccctgctgg catcgtgcgt gtgttcagcg ggaccccacc accacctgtc ctcagcttcc 2101 ggcttgtaca cacaaccgct attgagcact tccagcccaa cgccgatctg ctgttcagtg 2161 acccctccca gagtgaccct gagaccaaag acttctggct caacatggca gctctgaccg 2221 aagccctgca gcgccaggca gagcagaacc ccactgcctc ctactacaac gtggtgctgc 2281 tgcgatacca gttctcccgc ccgggtcccc agtctgtgcc tctgcagctc agtgcccact 2341 ggcagtgtgg agccaccctc acccaggtct cagtggagta cggctaccgg cccggtgcca 2401 cggctgtgcc cacaccactc acgaacgtcc agatcctgct gcctgtgggg gagcctgtga 2461 ccaacgtccg cttgcagccg gctgccacct ggaacctgga ggagaagcgg ctcacttgga 2521 ggcttccaga tgtgtccgag gcaggcggtt ctggccgcct ctctgccagc tgggagccgc 2581 tctcagggcc cagcacaccc agccccgtgg ctgcacagtt caccagcgag gggaccactc 2641 tgtcgggcgt ggacttggaa ctggtgggca gcggttaccg catgtcgctg gtgaagagga 2701 ggtttgccac agggatgtac ctggtgagct gctgcccaac tttcttgtac aaagtggttg 2761 gtaagcctat ccctaaccct ctcctcggtc tcgattctac gtagtaatga gctagcgcta 2821 accggtggcg cgttaagtcg acaatcaacc tctggatta