Construct: ORF ccsbBroad304_07857

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF005625.1_s304c1, BRDN0000461735
Derived from:
ccsbBroadEn_07857
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
RHOBTB2 (23221)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_07857
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 23221 RHOBTB2 Rho related BTB domain cont... NM_015178.2 99.9% 100% 864T>C
2 human 23221 RHOBTB2 Rho related BTB domain cont... NM_001160037.1 99% 99% 1_21del;885T>C
3 human 23221 RHOBTB2 Rho related BTB domain cont... NM_001160036.2 97% 97% 1_66del;930T>C
4 human 23221 RHOBTB2 Rho related BTB domain cont... XM_017013251.1 97% 97% 1_66del;930T>C
5 mouse 246710 Rhobtb2 Rho-related BTB domain cont... NM_153514.5 90.2% 97.5% (many diffs)
6 mouse 246710 Rhobtb2 Rho-related BTB domain cont... XM_017316022.1 87.6% 94.6% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
2247
ORF length:
2181
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgga ttctgacatg gattatgaaa ggccaaacgt agagaccatc aagtgcgttg
121tggtggggga caacgccgtg ggtaagacca ggctcatctg tgcccgcgct tgcaatgcca
181ccctcaccca gtaccagctg ctggccacgc atgtgcccac agtatgggcc atcgaccaat
241atcgtgtgtg ccaggaggtg ctggaacgct cccgagacgt ggtagatgat gtcagcgtct
301ctctgcgcct ctgggacacc tttggagacc accacaaaga ccgtcgcttt gcttatggga
361gatctgatgt ggtggttctg tgcttctcca ttgccaaccc caattccctc caccatgtca
421agaccatgtg gtacccagaa atcaagcact tctgcccccg agcacctgtc atcttggtgg
481gctgccagtt ggacctgcgc tacgctgacc tggaggctgt caacagggct aggcgaccct
541tggctaggcc catcaaacct aatgaaatcc tgcccccaga gaagggtcgg gaggtggcca
601aggagctggg catcccctac tatgagacca gcgtggtggc ccagttcggc atcaaggacg
661tctttgacaa cgccatccga gctgcactca tctcccgccg ccacctgcag ttctggaagt
721cccacctccg caatgtgcag cggcctctgc tgcaggcacc cttcctaccc cccaagccac
781cgcccccgat catcgtggtg cccgaccctc cctccagcag cgaggagtgc cccgcccacc
841tcctggagga cccgctctgc gcggacgtca tcctggtgct gcaggagcgg gtgcgcatct
901ttgcccacaa gatctacctc tccacctcct cctccaagtt ctatgacctg ttcctcatgg
961acctgagtga gggggagctg gggggcccct cggagccagg gggcacccac ccagaggacc
1021accagggcca ctctgatcaa caccaccacc atcaccacca ccaccatggg cgagacttcc
1081tgctccgagc agccagcttt gacgtgtgcg agagcgtgga tgaggctggg ggctccggtc
1141ctgctggcct ccgtgcttcc accagcgacg ggatcttacg gggcaacgga acagggtacc
1201taccgggcag gggtcgtgtg ctgtcttcct ggagccgagc ttttgtgagc atccaggaag
1261agatggcaga agatcctctc acctacaaat cccggctgat ggtggtggtg aagatggaca
1321gttccatcca gccggggccc ttccgggctg tcctcaagta cctgtacacg ggggagctag
1381atgagaacga gcgtgacctc atgcacattg cccacattgc tgagctgctc gaggtctttg
1441atctgcgcat gatggtggcc aacattctca acaatgaggc cttcatgaac caggagatca
1501ccaaggcctt ccacgtccgc cggaccaacc gggttaagga gtgcttggca aaaggcacct
1561tctcagatgt gaccttcatc ctggatgatg gcaccatcag cgcccacaag cccctgttga
1621tttccagctg tgactggatg gctgccatgt ttggggggcc atttgtggag agctccaccc
1681gggaggtggt gtttccctac acaagcaaga gctgcatgcg ggccgtgctg gaatacctct
1741acaccggcat gttcacctcc agccccgacc tggatgacat gaagctcatc attctagcca
1801accgcctctg cctgccacac ctggttgccc tcacagagca gtacacagtg accgggctga
1861tggaagcgac ccagatgatg gtggacatcg atggggacgt ccttgtgttc ctggaactgg
1921ctcagttcca ctgtgcgtac cagctggccg actggtgtct ccaccacatc tgcaccaact
1981acaacaacgt gtgccgcaag ttcccccgag acatgaaggc catgtcccca gaaaaccagg
2041agtatttcga gaagcatcgg tggccacctg tgtggtacct gaaggaggaa gatcattacc
2101agcgggcacg gaaggagcgt gagaaggagg actacctcca cctcaagcgg cagcccaaac
2161ggcgttggct cttctggaac agtccatcct ccccgtcttc ctcggcagcc tcctcctcat
2221ccccatcttc ctcctcggct gtggtctgcc caactttctt gtacaaagtg gttggtaagc
2281ctatccctaa ccctctcctc ggtctcgatt ctacgtagta atgagctagc gctaaccggt
2341ggcgcgttaa gtcgacaatc aacctctgga tta

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