Construct: ORF ccsbBroad304_07876

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF004382.1_s304c1, BRDN0000407224
Derived from:
ccsbBroadEn_07876
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
LARS2 (23395)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_07876
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 23395 LARS2 leucyl-tRNA synthetase 2, m... NM_001368263.1 99.8% 100% (many diffs)
2 human 23395 LARS2 leucyl-tRNA synthetase 2, m... NM_015340.4 99.8% 100% (many diffs)
3 human 23395 LARS2 leucyl-tRNA synthetase 2, m... XM_011533554.2 90.7% 87.7% (many diffs)
4 human 23395 LARS2 leucyl-tRNA synthetase 2, m... XM_017006042.1 88.7% 88.7% (many diffs)
5 mouse 102436 Lars2 leucyl-tRNA synthetase, mit... NM_001348167.1 85% 86.4% (many diffs)
6 mouse 102436 Lars2 leucyl-tRNA synthetase, mit... NM_153168.3 85% 86.4% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
2775
ORF length:
2709
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatggc ttctgtttgg cagagattgg gtttttatgc ctctcttctg aaaagacagc
121taaatggtgg gccagatgtc atcaagtggg aaaggagagt aattcccgga tgtaccagaa
181gcatctacag tgccacggga aagtggacaa aagagtatac attgcagaca agaaaggatg
241ttgagaaatg gtggcatcaa cgaataaaag aacaggcctc caaaatttca gaagctgata
301aatcgaagcc aaaattttac gtgctttcca tgttccctta tccttctggt aagctgcaca
361tgggccatgt gcgtgtctac accatcagcg acaccatagc acggttccag aagatgagag
421ggatgcaggt catcaacccc atgggatggg atgcttttgg attgcctgct gaaaatgccg
481cagtcgagag gaatctacat ccacaaagtt ggacacaaag taatattaaa cacatgagga
541aacagcttga tcgtctgggc ctgtgtttca gctgggatag ggaaataact acgtgtttgc
601cagattacta caagtggact cagtatctct ttattaaact gtatgaggct gggctggcct
661atcaaaagga ggccctggtt aactgggacc cagtggatca aacagtgctt gccaatgagc
721aggtggatga acatggctgt tcatggcgtt ctggagcaaa ggtggaacag aagtacctca
781gacaatggtt tattaagaca accgcttatg caaaggccat gcaggacgcg ttggcagacc
841ttccagaatg gtatggaata aaaggcatgc aagcccactg gattggggac tgtgtgggct
901gccacctgga cttcacatta aaggttcatg ggcaagccac gggcgaaaag ctgactgcct
961atacggccac ccctgaagcc atttatggca cctcccacgt ggccatctcg cccagccaca
1021gactcctaca tgggcacagc tctctgaagg aagccttgag gatggccctt gtccctggca
1081aagattgcct cacgcctgta atggctgtga acatgctcac ccagcaggag gtccctgtcg
1141ttattttggc caaagctgac ttggaaggct ctctggattc aaaaatagga attcccagta
1201ctagctcaga ggacaccatc ttagcccaaa ccctgggcct ggcctactct gaagtcattg
1261aaactttgcc agatggcaca gagagactga gcagctctgc tgagttcaca ggtatgaccc
1321ggcaggatgc ttttctagcc ctgactcaga aagcccgggg gaagagagtg ggtggagacg
1381tgacaagtga taaactgaaa gactggctga tttcacggca gcggtactgg ggcacaccaa
1441tccccattgt ccactgccca gtctgtggcc ccacacctgt gcccctggag gacttgcctg
1501tgaccctgcc caacatcgca tctttcactg gcaagggagg ccccccactg gccatggctt
1561cagagtgggt gaactgctcc tgcccaaggt gcaagggagc agccaagaga gagacagaca
1621cgatggatac ctttgttgat tctgcttggt actacttcag atacactgac cctcataatc
1681cacacagccc ttttaacaca gcagtggccg attactggat gcctgtggat ttgtacattg
1741gagggaaaga acatgccgtc atgcacttgt tctatgcaag attctttagt catttttgcc
1801atgatcaaaa aatggttaaa catagggagc cttttcataa gctgctggcc caaggcctta
1861tcaaggggca gacattccgc ctaccatctg gacagtatct acagagagag gaagtggatc
1921tcacaggttc cgttcctgtt catgcaaaaa cgaaagagaa gttagaggtg acgtgggaga
1981agatgagtaa gtccaaacac aacggggtgg acccagagga agttgtggag cagtatggga
2041tcgacacgat tcggctctac atcctttttg ctgcccctcc tgagaaggat atcttgtggg
2101atgtgaaaac tgatgctctc cctggggtgc tgagatggca acaacgactg tggaccttga
2161caactcggtt tattgaggcc agggcttctg ggaagtctcc ccagcctcag ctgctgagta
2221acaaggagaa agccgaggcc aggaagctct gggagtacaa gaactccgtc atctctcagg
2281tgaccaccca tttcacagag gacttctcac tgaattctgc aatttctcag ctgatgggac
2341tcagcaatgc cctctcgcaa gcctctcaga gcgtcattct ccacagcccc gagtttgagg
2401atgctttgtg tgccctgatg gtgatggctg ctccactggc ccctcatgta acctcagaga
2461tctgggcagg cctggcgctg gtgccgagga agctctgtgc ccactacact tgggatgcca
2521gtgtgctgct ccaggcatgg cctgctgtgg acccggagtt cctgcagcag cctgaggttg
2581tccagatggc agttctgatc aacaataaag cttgtggcaa aattcctgtg ccccaacaag
2641ttgcccggga ccaggacaaa gtccacgaat ttgttcttca aagcgagctg ggtgtcaggc
2701ttttgcaagg acgaagcatc aagaagtcct tcctttcccc gagaactgcc ctcatcaact
2761tcctggtgca agattgccca actttcttgt acaaagtggt tggtaagcct atccctaacc
2821ctctcctcgg tctcgattct acgtagtaat gagctagcgc taaccggtgg cgcgttaagt
2881cgacaatcaa cctctggatt a

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