Construct: ORF ccsbBroad304_07933
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010018.1_s304c1, BRDN0000406531
- Derived from:
- ccsbBroadEn_07933
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- APOL2 (23780)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_07933
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 23780 | APOL2 | apolipoprotein L2 | NM_030882.4 | 99.9% | 99.7% | 6C>N |
| 2 | human | 23780 | APOL2 | apolipoprotein L2 | NM_145637.2 | 99.9% | 99.7% | 6C>N |
| 3 | human | 23780 | APOL2 | apolipoprotein L2 | XM_011530074.1 | 99.9% | 99.7% | 6C>N |
| 4 | human | 23780 | APOL2 | apolipoprotein L2 | XM_011530075.1 | 99.9% | 99.7% | 6C>N |
| 5 | human | 23780 | APOL2 | apolipoprotein L2 | XM_011530076.3 | 99.9% | 99.7% | 6C>N |
| 6 | human | 23780 | APOL2 | apolipoprotein L2 | XM_011530077.3 | 99.9% | 99.7% | 6C>N |
| 7 | human | 23780 | APOL2 | apolipoprotein L2 | XM_011530078.1 | 99.9% | 99.7% | 6C>N |
| 8 | human | 23780 | APOL2 | apolipoprotein L2 | XM_017028724.1 | 74.9% | 74.8% | 1_336del;342C>N |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1077
- ORF length:
- 1011
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgaa nccagagagc agtatcttta ttgaggatta ccttaagtat ttccaggacc 121 aagtgagcag agagaatctg ctacaactgc tgactgatga tgaagcctgg aatggattcg 181 tggctgctgc tgaactgccc agggatgagg cagatgagct ccgtaaagct ctgaacaagc 241 ttgcaagtca catggtcatg aaggacaaaa accgccacga taaagaccag cagcacaggc 301 agtggttttt gaaagagttt cctcggttga aaagggagct tgaggatcac ataaggaagc 361 tccgtgccct tgcagaggag gttgagcagg tccacagagg caccaccatt gccaatgtgg 421 tgtccaactc tgttggcact acctctggca tcctgaccct cctcggcctg ggtctggcac 481 ccttcacaga aggaatcagt tttgtgctct tggacactgg catgggtctg ggagcagcag 541 ctgctgtggc tgggattacc tgcagtgtgg tagaactagt aaacaaattg cgggcacgag 601 cccaagcccg caacttggac caaagcggca ccaatgtagc aaaggtgatg aaggagtttg 661 tgggtgggaa cacacccaat gttcttacct tagttgacaa ttggtaccaa gtcacacaag 721 ggattgggag gaacatccgt gccatcagac gagccagagc caaccctcag ttaggagcgt 781 atgccccacc cccgcatgtc attgggcgaa tctcagctga aggcggtgaa caggttgaga 841 gggttgttga aggccccgcc caggcaatga gcagaggaac catgatcgtg ggtgcagcca 901 ctggaggcat cttgcttctg ctggatgtgg tcagccttgc atatgagtca aagcacttgc 961 ttgagggggc aaagtcagag tcagctgagg agctgaagaa gcgggctcag gagctggagg 1021 ggaagctcaa ctttctcacc aagatccatg agatgctgca gccaggccaa gaccaatgcc 1081 caactttctt gtacaaagtg gttggtaagc ctatccctaa ccctctcctc ggtctcgatt 1141 ctacgtagta atgagctagc gctaaccggt ggcgcgttaa gtcgacaatc aacctctgga 1201 tta