Construct: ORF ccsbBroad304_07944
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012537.1_s304c1, BRDN0000411044
- Derived from:
- ccsbBroadEn_07944
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- BRI3 (25798)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_07944
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 25798 | BRI3 | brain protein I3 | NM_015379.5 | 99.7% | 99.2% | 157C>T |
| 2 | human | 25798 | BRI3 | brain protein I3 | XM_017011936.1 | 75.5% | 57.7% | (many diffs) |
| 3 | human | 25798 | BRI3 | brain protein I3 | NM_001159491.2 | 74.9% | 62.4% | (many diffs) |
| 4 | human | 25798 | BRI3 | brain protein I3 | XM_017011935.2 | 66.3% | 34.3% | (many diffs) |
| 5 | human | 25798 | BRI3 | brain protein I3 | XM_017011932.2 | 65.5% | 31.5% | (many diffs) |
| 6 | human | 25798 | BRI3 | brain protein I3 | XM_017011933.2 | 65.5% | 31.5% | (many diffs) |
| 7 | human | 25798 | BRI3 | brain protein I3 | XM_017011934.2 | 65.5% | 31.5% | (many diffs) |
| 8 | human | 25798 | BRI3 | brain protein I3 | XM_017011931.2 | 63.2% | 30.5% | (many diffs) |
| 9 | mouse | 55950 | Bri3 | brain protein I3 | NM_018772.4 | 89.3% | 92.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 441
- ORF length:
- 375
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgga ccacaagccg ctgctgcagg agcggccgcc cgcctacaac ctggaggccg 121 gccagggcga ctacgcgtgc ggcccgcacg gctacggcgc catccccgcc gcgcccccgc 181 cgccgcccta cccctacctc gtcacaggga tacccaccca ctatcccagg gtctacaaca 241 tccacagccg gaccgtcacc cgctatcctg ccaactctat cgtggtcgta ggaggctgtc 301 ctgtctgcag ggttggggtg ctggaggact gcttcacctt cctgggcatc ttcctggcca 361 tcatcctctt cccctttggg ttcatttgct gttttgcctt gaggaagcga cgatgcccca 421 actgtggagc caccttcgct tacccaactt tcttgtacaa agtggttggt aagcctatcc 481 ctaaccctct cctcggtctc gattctacgt agtaatgagc tagcgctaac cggtggcgcg 541 ttaagtcgac aatcaacctc tggatta