Construct: ORF ccsbBroad304_07960

Construct Description:

• Construct Type: ORF
• Other Identifiers: ORF000558.1_s304c1, BRDN0000462142
• Derived from: ccsbBroadEn_07960
• DNA Barcode: None
• Epitope Tag: V5
• Notes: No stop codon in insert

Originally Annotated References:

• Gene: RNF19A (25897)

Vector Information:

• Vector Backbone: pLX_304
• Pol II Cassette 1: PGK-BlastR
• Pol II Cassette 2: CMV-ccsbBroad304_07960
• Selection Marker: BlastR
• Visible Reporter: n/a
• Epitope Tag: V5

Current transcripts matched by this ORF:

	Taxon	Gene	Symbol	Description	Transcript	Nuc. Match %	Prot. Match %	Match Diffs
1	human	25897	RNF19A	ring finger protein 19A, RB...	NM_001280539.2	99.9%	99.7%	889G>T;1435G>T
2	human	25897	RNF19A	ring finger protein 19A, RB...	NM_001353837.1	99.9%	99.7%	889G>T;1435G>T
3	human	25897	RNF19A	ring finger protein 19A, RB...	NM_001353838.2	99.9%	99.7%	889G>T;1435G>T
4	human	25897	RNF19A	ring finger protein 19A, RB...	NM_015435.4	99.9%	99.7%	889G>T;1435G>T
5	human	25897	RNF19A	ring finger protein 19A, RB...	NM_183419.4	99.9%	99.7%	889G>T;1435G>T
6	human	25897	RNF19A	ring finger protein 19A, RB...	XM_017013302.1	99.9%	99.7%	889G>T;1435G>T
7	human	25897	RNF19A	ring finger protein 19A, RB...	XM_017013303.2	99.9%	99.7%	889G>T;1435G>T
8	human	25897	RNF19A	ring finger protein 19A, RB...	XM_024447118.1	99.9%	99.7%	889G>T;1435G>T
9	human	25897	RNF19A	ring finger protein 19A, RB...	XM_024447119.1	99.9%	99.7%	889G>T;1435G>T
10	human	25897	RNF19A	ring finger protein 19A, RB...	XM_011516956.3	93.5%	93.4%	889G>T;1300_1301ins162
11	human	25897	RNF19A	ring finger protein 19A, RB...	XM_017013304.1	93.5%	93.4%	889G>T;1300_1301ins162
12	human	25897	RNF19A	ring finger protein 19A, RB...	XM_024447120.1	62.9%	62.8%	0_1ins930;505G>T
13	human	25897	RNF19A	ring finger protein 19A, RB...	XR_928315.3	53%		(many diffs)
14	mouse	30945	Rnf19a	ring finger protein 19A	NM_013923.2	88.4%	93.3%	(many diffs)
15	mouse	30945	Rnf19a	ring finger protein 19A	XM_006520081.3	59.9%	56.6%	(many diffs)

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:

69

ORF end:

2583

ORF length:

2514

Sequence:

1	ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61	ttggcaccat gcaagaacaa gaaataggtt ttatctctaa atataatgaa gggctgtgtg
121	taaacactga ccctgtctca attctaacaa gcattttaga catgagttta catcggcaaa
181	tgggttcaga tcgagatctt cagtcctctg cttcatctgt gagcttgcct tcagtcaaaa
241	aggcacccaa aaaaagaaga atttcaatag gctccctgtt tcggaggaaa aaagataaca
301	aacgtaaatc aagggagcta aatggcgggg tggatggaat tgcaagtatt gaaagtatac
361	attctgaaat gtgtactgat aagaactcca ttttctctac aaatacctct tctgacaatg
421	gattaacttc catcagcaaa caaattggag acttcataga gtgccctttg tgccttttgc
481	ggcattctaa agacagattt cctgatataa tgacttgtca tcacagatct tgtgtggatt
541	gcttacgaca atatttaagg atagaaatct ctgaaagcag agttaatatt agttgcccag
601	aatgtactga acggtttaat ccccatgata ttcgcttgat attaagtgat gatgtcttga
661	tggaaaaata cgaagaattt atgcttagac ggtggcttgt tgcagatcct gattgtaggt
721	ggtgtccagc tccagactgt ggatatgctg tgatagcatt tggatgtgcc agctgtccaa
781	aattaacttg tgggcgagag ggctgtggaa cagagttttg ctaccactgt aaacagattt
841	ggcaccccaa ccagacctgt gatgctgctc gacaagagag agcccagagc ttacgtttga
901	gaactatacg ttcttcatcc attagttata gtcaagagtc tggagcagca gctgattata
961	taaagccatg tccacgatgt gctgcttata taataaagat gaatgatggg agctgcaatc
1021	acatgacatg tgctgtttgt ggttgtgagt tttgttggtt gtgtatgaaa gaaatctcag
1081	atttgcatta tctaagtcca tcaggatgta ctttttgggg gaagaaaccc tggagccgaa
1141	agaagaaaat attgtggcaa ctgggaacac tggttggtgc tcctgtcgga atcgctttaa
1201	tagctggcat tgctattcct gcaatgatta ttggcattcc tgtgtatgtg ggccgcaaga
1261	ttcacaatcg ctatgaaggc aaggatgttt caaagcacaa acggaatttg gccatagcag
1321	gtggtgtaac gttgtctgta atcgtgtctc cagtagtagc tgcagtgact gtaggtatcg
1381	gtgttcctat tatgttagct tatgtctatg gcgtagttcc aatttctctt tgtcgaagcg
1441	gaggttgtgg agtctcagca ggcaatggaa aaggagttag gattgaattt gatgatgaaa
1501	attatataaa tgttggtgga actaacacag ctgtagacac aacatcagta gcagaagcaa
1561	gacacaaccc aagcataggg gagggaagtg ttggtgggct gactggcagt ttgagtgcaa
1621	gtggaagcca catggatcga ataggagcca tccgagacaa cctgagtgaa acggccagca
1681	ccatggcact agctggagcc agtataacgg ggagtctgtc aggaagtgcc atggtaaact
1741	gttttaacag gttggaagta caagcagatg tacagaaaga acggtacagt ctaagtggag
1801	aatctggcac agtcagcttg ggaacagtta gtgataatgc cagcaccaaa gcaatggcag
1861	gatccattct gaattcctac atcccattgg acaaagaagg caacagtatg gaggtgcaag
1921	tagatattga gtcaaagcca tccaaattca ggcacaacag tggaagcagt agtgtggatg
1981	atggcagtgc cacccgaagt catgctggcg gttcatccag tggcttgcct gaaggtaaat
2041	ctagtgccac caagtggtcc aaagaagcaa cagcagggaa aaaatcaaaa agtggtaaac
2101	tgaggaaaaa gggtaacatg aagataaatg agacgagaga ggacatggat gcacagttgt
2161	tagaacaaca aagcacgaac tcaagtgaat ttgaggctcc atccctcagt gacagtatgc
2221	cttctgtagc agattctcac tctagtcatt tttctgaatt tagttgttct gacctagaaa
2281	gcatgaaaac ttcttgtagt catggttcca gtgattatca cacccgcttt gctactgtta
2341	acattcttcc tgaggtagaa aatgaccgtc tggaaaattc cccacatcag tgtagcattt
2401	ctgtggttac ccaaactgct tcctgttcag aagtttcaca gttgaatcat attgctgaag
2461	aacatggtaa caatggaata aaacctaatg ttgatttata ttttggcgat gcactaaaag
2521	aaacaaataa caaccactca catcagacaa tggaattaaa agttgcaatt cagactgaaa
2581	ttttgccaac tttcttgtac aaagtggttg gtaagcctat ccctaaccct ctcctcggtc
2641	tcgattctac gtagtaatga gctagcgcta accggtggcg cgttaagtcg acaatcaacc
2701	tctggatta

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