Construct: ORF ccsbBroad304_07970

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF009893.1_s304c1, BRDN0000402037
Derived from:
ccsbBroadEn_07970
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
RWDD3 (25950)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_07970
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 25950 RWDD3 RWD domain containing 3 NM_001128142.1 99.6% 98.9% 226A>G;258T>G
2 human 25950 RWDD3 RWD domain containing 3 NM_001199682.1 90.3% 86.8% (many diffs)
3 human 25950 RWDD3 RWD domain containing 3 NM_001278247.1 81.2% 71.6% (many diffs)
4 human 25950 RWDD3 RWD domain containing 3 NM_015485.5 72.5% 70.7% (many diffs)
5 human 25950 RWDD3 RWD domain containing 3 NM_001278248.1 65.6% 59.8% (many diffs)
6 human 25950 RWDD3 RWD domain containing 3 NR_103483.1 46% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
654
ORF length:
585
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcaccat ggcggagcct gtgcaggagg agctctcggt cctggccgcg attttctgca
121ggccccacga gtgggaggtg ctgagccgct cagagacaga tgggaccgtg ttcagaattc
181acacaaaagc tgaaggattt atggatgcgg atatacctct ggaattggtg ttccatttgc
241cagtcaatta tccttcatgt ctacctggta tctcgattaa ctctgaacag ttggccaggg
301cccagtgtgt gactgtgaaa gagaagttac ttgagcaagc agagagcctt ttgtcggagc
361ctatggttca tgagctggtt ctctggattc agcagaatct caggcatatc ctcagccaac
421cagaaactgg cagtggcagt gaaaagtgta ctttttcaac aagcacgacc atggatgatg
481gattgtggat aactcttttg catttagatc acatgagagc aaagactaaa tatgtcaaaa
541ttgtggagaa gtgggcttca gatttaaggc tgacaggaag actgatgttc atgggtaaaa
601taatactgat tttactacag ggagacagaa acaacctcaa ggtgccaaaa agtttgccaa
661ctttcttgta caaagtggtt ggtaagccta tccctaaccc tctcctcggt ctcgattcta
721cgtagtaatg agctagcgct aaccggtggc gcgttaagtc gacaatcaac ctctggatta

Download FASTA (ORF) (Full)