Transcript: Human NM_001278248.1

Homo sapiens RWD domain containing 3 (RWDD3), transcript variant 5, mRNA.

Source:
NCBI, updated 2019-08-03
Taxon:
Homo sapiens (human)
Gene:
RWDD3 (25950)
Length:
1383
CDS:
255..1013

Additional Resources:

NCBI RefSeq record:
NM_001278248.1
NBCI Gene record:
RWDD3 (25950)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001278248.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000004094 GATGGTAAATGAACACTTGTT pLKO.1 1271 3UTR 100% 4.950 3.465 N RWDD3 n/a
2 TRCN0000433555 AGAAGTGGGCTTCAGATTTAA pLKO_005 688 CDS 100% 15.000 7.500 Y RWDD3 n/a
3 TRCN0000004095 ACATGAGAGCAAAGACTAAAT pLKO.1 652 CDS 100% 13.200 6.600 Y RWDD3 n/a
4 TRCN0000432027 GATTTATGGATGCGGATATAC pLKO_005 337 CDS 100% 13.200 6.600 Y RWDD3 n/a
5 TRCN0000004096 TGGATGATGGATTGTGGATAA pLKO.1 613 CDS 100% 10.800 5.400 Y RWDD3 n/a
6 TRCN0000004093 CCAGTCAATTATCCTTCATGT pLKO.1 381 CDS 100% 4.950 2.475 Y RWDD3 n/a
7 TRCN0000418354 TTGTCGGAGCCTATGGTTCAT pLKO_005 492 CDS 100% 4.950 2.475 Y RWDD3 n/a
8 TRCN0000004092 CATATCCTCAGCCAACCAGAA pLKO.1 546 CDS 100% 4.050 2.025 Y RWDD3 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001278248.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_15777 pDONR223 0% 73.2% 67.4% None 213T>G;481_527del;603_756del n/a
2 ccsbBroad304_15777 pLX_304 0% 73.2% 67.4% V5 213T>G;481_527del;603_756del n/a
3 TRCN0000465451 ACCGAGGTCTATATCCGGGTCAAT pLX_317 46.3% 73.2% 67.4% V5 213T>G;481_527del;603_756del n/a
4 ccsbBroadEn_07970 pDONR223 100% 65.6% 59.8% None (many diffs) n/a
5 ccsbBroad304_07970 pLX_304 0% 65.6% 59.8% V5 (many diffs) n/a
6 TRCN0000469422 TGTCGGGTTGATTCAGGCCTCGAT pLX_317 65.9% 65.6% 59.8% V5 (many diffs) n/a
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