Construct: ORF ccsbBroad304_07978
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004377.1_s304c1, BRDN0000403861
- Derived from:
- ccsbBroadEn_07978
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- L3MBTL1 (26013)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_07978
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 26013 | L3MBTL1 | L3MBTL histone methyl-lysin... | NM_015478.7 | 99.9% | 99.8% | 146G>C |
| 2 | human | 26013 | L3MBTL1 | L3MBTL histone methyl-lysin... | NM_032107.5 | 90.8% | 88.1% | (many diffs) |
| 3 | mouse | 241764 | L3mbtl1 | l(3)mbt-like (Drosophila) | XM_011239550.2 | 69.9% | 66.4% | (many diffs) |
| 4 | mouse | 241764 | L3mbtl1 | l(3)mbt-like (Drosophila) | XM_011239552.2 | 45.5% | 43.9% | (many diffs) |
| 5 | mouse | 241764 | L3mbtl1 | l(3)mbt-like (Drosophila) | XM_011239553.2 | 45.5% | 43.9% | (many diffs) |
| 6 | mouse | 241764 | L3mbtl1 | l(3)mbt-like (Drosophila) | XR_001782609.1 | 27% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2382
- ORF length:
- 2316
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgag gcgaagagag ggccatggca ccgactccga gatgggtcaa ggacccgtac 121 gggagtcgca atcctcagac cctcccgcgc tccagttccg gataagcgag tataagccgc 181 tgaacatggc gggagtggag cagcccccga cccccgagct gcggcaggaa ggcgtgaccg 241 aatacgaaga tggcggggcc ccggcgggag atggcgaggc gggcccccaa caggcggagg 301 accaccccca gaatcctcca gaagatccca atcaggaccc cccagaggat gatagcacct 361 gtcagtgcca ggcgtgcggg cctcaccaag ccgcgggtcc agatcttggt tcctctaatg 421 atggctgccc tcagctgttc caggagcggt cagtcatagt ggagaactcc tcaggctcta 481 ccagcgcttc tgagctcctc aaacccatga agaagaggaa gcgcagggaa taccagagcc 541 catcagagga ggagtcggag ccagaggcca tggagaagca agaagaagga aaggacccag 601 agggacaacc cactgctagc accccagaga gtgaggagtg gagcagcagc cagcctgcaa 661 caggtgagaa gaaggaatgc tggtcgtggg agtcctacct agaggagcag aaggccatta 721 ctgctccagt cagcctcttc caggactccc aggcagtcac tcacaacaag aatggcttca 781 aactgggcat gaagttggaa ggcattgacc ctcaacaccc gtccatgtac ttcatcctca 841 ccgtggctga ggtatgtggc tatcgcctac gcctgcactt tgatgggtat tctgagtgcc 901 atgacttctg ggtcaatgcc aactcccctg acattcaccc tgctggctgg ttcgagaaga 961 cgggccacaa gctgcagcct cccaaaggtt acaaggagga ggagttcagc tggagccagt 1021 acctgcgcag cacaagagct caggctgccc ccaagcacct gtttgtgagc cagagccaca 1081 gtcccccacc cctgggcttc caggtgggca tgaagctgga ggctgttgac cgcatgaacc 1141 cgtcccttgt ctgcgtggcc agtgtgaccg atgtggtgga cagccgcttc ctggtgcact 1201 ttgacaactg ggatgatact tatgactact ggtgtgatcc cagcagcccc tacatccacc 1261 cagtgggctg gtgccagaag caaggaaagc ccctcacccc tccacaagac tacccagacc 1321 ctgataactt ctgttgggag aaatatctgg aagaaactgg ggcctctgct gtccccacct 1381 gggccttcaa ggtgcgaccc cctcacagct tcctggtcaa tatgaagctg gaggctgtgg 1441 accgcaggaa cccagccctg attcgcgtgg ccagcgtgga ggatgtggag gaccatcgga 1501 taaagatcca ctttgatggc tggagtcatg gctatgattt ctggatcgac gctgaccacc 1561 cagacatcca ccctgccggc tggtgctcca agacaggaca tcccctgcag cctcctctcg 1621 gacccagaga gcccagctct gcctcccctg ggggctgtcc ccctctcagc tataggagcc 1681 tgccccacac taggacctcc aaatacagct ttcaccaccg gaagtgcccc actcctggtt 1741 gcgacggctc tggccatgtc acaggcaagt tcacagctca ccattgcctc tcaggctgcc 1801 cactggctga gaggaaccag agccggctga aagcggagct gtctgactcg gaggcctcag 1861 cccgcaagaa gaacctctca ggcttctccc caaggaagaa gcctcgccat cacggccgaa 1921 ttggacgccc tccgaagtat cgaaagattc cgcaggaaga tttccagacc ctcacgcccg 1981 atgtcgtgca ccagtccctc ttcatgtcag ccctgtcggc ccaccctgac cgctcactct 2041 cagtgtgctg ggagcagcac tgcaagctcc tgccaggagt agcgggcatc tcagcctcga 2101 cagtcgccaa gtggaccatc gatgaggtct tcggctttgt tcagaccctg acaggttgtg 2161 aggaccaagc acgcctcttc aaagacgagg caagaatagt cagagtgacc catgtatctg 2221 ggaagactct agtctggact gtggcccagc ttggggacct tgtgtgctca gatcatcttc 2281 aggaaggaaa aggcatcctg gagacaggag tccattcact cctctgctct ctacccactc 2341 atttgcttgc caaacttagc tttgccagtg atagtcaata ttacccaact ttcttgtaca 2401 aagtggttgg taagcctatc cctaaccctc tcctcggtct cgattctacg tagtaatgag 2461 ctagcgctaa ccggtggcgc gttaagtcga caatcaacct ctggatta