Construct: ORF ccsbBroad304_07983
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001728.1_s304c1, BRDN0000410853
- Derived from:
- ccsbBroadEn_07983
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RAI14 (26064)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_07983
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 26064 | RAI14 | retinoic acid induced 14 | NM_001145520.1 | 99.8% | 99.7% | (many diffs) |
| 2 | human | 26064 | RAI14 | retinoic acid induced 14 | NM_001145521.1 | 99.8% | 99.7% | (many diffs) |
| 3 | human | 26064 | RAI14 | retinoic acid induced 14 | NM_015577.3 | 99.8% | 99.7% | (many diffs) |
| 4 | human | 26064 | RAI14 | retinoic acid induced 14 | XM_006714469.2 | 99.8% | 99.7% | (many diffs) |
| 5 | human | 26064 | RAI14 | retinoic acid induced 14 | XM_011514017.2 | 99.8% | 99.7% | (many diffs) |
| 6 | human | 26064 | RAI14 | retinoic acid induced 14 | XM_011514018.1 | 99.8% | 99.7% | (many diffs) |
| 7 | human | 26064 | RAI14 | retinoic acid induced 14 | XM_011514019.1 | 99.8% | 99.7% | (many diffs) |
| 8 | human | 26064 | RAI14 | retinoic acid induced 14 | XM_011514020.1 | 99.8% | 99.7% | (many diffs) |
| 9 | human | 26064 | RAI14 | retinoic acid induced 14 | XM_011514021.2 | 99.8% | 99.7% | (many diffs) |
| 10 | human | 26064 | RAI14 | retinoic acid induced 14 | XM_011514022.1 | 99.8% | 99.7% | (many diffs) |
| 11 | human | 26064 | RAI14 | retinoic acid induced 14 | NM_001145523.1 | 98.9% | 98.5% | (many diffs) |
| 12 | human | 26064 | RAI14 | retinoic acid induced 14 | NM_001145525.1 | 98.8% | 98.4% | (many diffs) |
| 13 | human | 26064 | RAI14 | retinoic acid induced 14 | XM_011514016.2 | 98.5% | 98.5% | (many diffs) |
| 14 | human | 26064 | RAI14 | retinoic acid induced 14 | XM_017009334.1 | 98.4% | 90.5% | (many diffs) |
| 15 | human | 26064 | RAI14 | retinoic acid induced 14 | XM_011514023.1 | 97.9% | 91.4% | (many diffs) |
| 16 | human | 26064 | RAI14 | retinoic acid induced 14 | XM_011514024.2 | 97.9% | 91.4% | (many diffs) |
| 17 | human | 26064 | RAI14 | retinoic acid induced 14 | XM_024446017.1 | 97.9% | 91.4% | (many diffs) |
| 18 | human | 26064 | RAI14 | retinoic acid induced 14 | NM_001145522.2 | 96.9% | 96.8% | (many diffs) |
| 19 | human | 26064 | RAI14 | retinoic acid induced 14 | XM_017009335.1 | 96.9% | 96.8% | (many diffs) |
| 20 | human | 26064 | RAI14 | retinoic acid induced 14 | XM_011514025.2 | 95.6% | 95.6% | (many diffs) |
| 21 | human | 26064 | RAI14 | retinoic acid induced 14 | XM_017009336.2 | 95% | 88.5% | (many diffs) |
| 22 | mouse | 75646 | Rai14 | retinoic acid induced 14 | NM_001166408.1 | 83.5% | 85.1% | (many diffs) |
| 23 | mouse | 75646 | Rai14 | retinoic acid induced 14 | NM_030690.3 | 83.5% | 85.1% | (many diffs) |
| 24 | mouse | 75646 | Rai14 | retinoic acid induced 14 | XM_006520187.2 | 83.5% | 85.1% | (many diffs) |
| 25 | mouse | 75646 | Rai14 | retinoic acid induced 14 | XM_006520188.3 | 83.5% | 85.1% | (many diffs) |
| 26 | mouse | 75646 | Rai14 | retinoic acid induced 14 | XM_017316775.1 | 83.5% | 85.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 3006
- ORF length:
- 2940
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgaa gagcttgaaa gcgaagttca ggaagagtga caccaatgag tggaacaaga 121 atgatgaccg gctactgcag gccgtggaga atggagatgc ggagaaggtg gcctcactgc 181 tcggcaagaa gggggccagt gccaccaaac acgacagtga gggcaagacc gctttccatc 241 ttgctgctgc aaaaggacac gtggaatgcc tcagggtcat gattacacat ggtgtggatg 301 tgacagccca agatactacc ggacacagcg ccttacatct cgcagccaag aacagccacc 361 atgaatgcat caggaagctg cttcagtcta aatgcccagc cgaaagtgtt gacagctctg 421 ggaaaacagc tttacattat gcagcggctc agggctgcct tcaagctgtg cagattctct 481 gcgaacacaa gagccccata aacctcaaag atttggatgg gaatataccg ctgctgcttg 541 ctgtacaaaa tggtcacagt gagatctgtc actttctcct ggatcatgga gcagatgtca 601 attccaggaa caaaagtgga agaactgctc tcatgctggc ctgtgagatt ggcagctcta 661 acgctgtgga agccttaatt aaaaagggtg cagacctaaa ccttgtagat tctcttggat 721 acaatgcctt acattattcc aaactctcag aaaatgcagg aattcaaagc cttctattat 781 caaaaatctc tcaggatgct gatttaaaga ccccaacaaa accaaagcag catgaccaag 841 tctctaaaat aagctcagaa agaagtggaa ctccaaaaaa acgcaaagct ccaccacctc 901 ctatcagtcc tacccagttg agtgatgtct cttccccaag atcaataact tcgactccac 961 tatcgggaaa ggaatcggta ttttttgctg aaccaccctt caaggctgag atcagttcta 1021 tacgagaaaa caaagacaga ctaagtgaca gtactacagg tgctgatagc ttattggata 1081 taagttctga agctgaccaa caagatcttc tctctctatt gcaagcaaaa gttgcttccc 1141 ttaccttaca caataaggag ttacaagata aattacaggc caaatcaccc aaggaggcgg 1201 aagcagacct aagctttgac tcataccatt ccacccaaac tgacttgggc ccatccctgg 1261 gaaaacctgg tgaaacctct cccccagact ccaaatcatc tccatctgtc ttaatacatt 1321 ctttaggtaa atccactact gacaatgatg tcagaattca gcaactgcaa gagattttgc 1381 aagatctaca gaagagatta gagagctctg aagcagagag aaaacagcta caggtcgaac 1441 tccaatcccg aagggcagaa ctggtatgct taaacaacac tgagatttca gagaacagct 1501 ctgacctcag ccagaaactt aaagaaactc agagcaaata cgaggaggct atgaaagaag 1561 tccttagtgt gcagaagcag atgaaactcg gtcttgtctc acctgaaagc atggataatt 1621 attcacattt ccacgagctg agggtcacgg aagaggaaat aaatgtgcta aagcaggatc 1681 tgcagaatgc attagaagaa agtgaaagaa ataaagagaa agtgagagag ttagaggaaa 1741 aactggtaga gagggagaaa ggtacagtga ttaagccacc tgtggaagag tacgaggaaa 1801 tgaaaagttc atattgctct gttattgaga atatgaataa ggagaaagca tttttgtttg 1861 agaaatacca agaagcccaa gaagaaatca tgaaattaaa agacacacta aaaagtcaga 1921 tgacacagga agccagtgat gaagctaagg acatgaaaga agccatgaat aggatgatag 1981 atgaactcaa taaacaggtg agcgagctgt cacagctgta caaagaagcc caggctgagc 2041 tggaggatta caggaagagg aaatctctag aggatgtcac agctgaatat atccataaag 2101 cagagcatga gaaactgatg caattgacaa acgtgtccag ggctaaagca gaagatgcac 2161 tgtctgaaat gaagtctcag tattcaaaag tgttgaatga gttgacccag ctcaaacaac 2221 tggtggatgc acaaaaagag aactctgtct ctatcacaga acatttgcaa gtgataacca 2281 cgctgcggac tgcagcaaaa gagatggaag aaaaaataag caatcttaag gaacaccttg 2341 caagcaagga agtggaagta gcaaagctgg agaaacaact cttagaagag aaagctgcta 2401 tgactgatgc aatggtacct cggtcttcct atgaaaaact ccagtcatcc ttagagagtg 2461 aagtgagtgt gttggcatcg aaattaaagg aatctgtgaa agagaaagag aaggtccatt 2521 cagaggttgt ccagattaga agtgaggtct cacaggtgaa aagagaaaag gaaaatattc 2581 agactctctt gaaatccaaa gagcaagaag taaatgaact tctgcaaaaa ttccagcaag 2641 ctcaggaaga acttgcagaa atgaaaagat actctgagag ctcttcaaaa ctggaggaag 2701 ataaagataa aaagataaat gagatgtcga aggaagtcac caaattgaag gaggccttga 2761 acagcctctc ccagctctcc tactcaacaa gctcatccaa aaggcagagt cagcagctgg 2821 aggcgctgca gcagcaagtc aaacagctcc agaaccagct ggcggaatgc aagaaacaac 2881 accaggaggt catatcagtt tacagaatgc atcttctgta tgctgtgcag ggccagatgg 2941 atgaagatgt ccagaaagta ctgaagcaaa tccttaccat gtgtaaaaac cagtctcaaa 3001 agaagtaccc aactttcttg tacaaagtgg ttggtaagcc tatccctaac cctctcctcg 3061 gtctcgattc tacgtagtaa tgagctagcg ctaaccggtg gcgcgttaag tcgacaatca 3121 acctctggat ta