Construct: ORF ccsbBroad304_07988
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015318.1_s304c1, BRDN0000410170
- Derived from:
- ccsbBroadEn_07988
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DCAF4 (26094)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_07988
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 26094 | DCAF4 | DDB1 and CUL4 associated fa... | NM_015604.4 | 99.7% | 99.3% | 578A>C;1000C>T;1316G>T |
| 2 | human | 26094 | DCAF4 | DDB1 and CUL4 associated fa... | XM_017021207.1 | 99.7% | 99.3% | 578A>C;1000C>T;1316G>T |
| 3 | human | 26094 | DCAF4 | DDB1 and CUL4 associated fa... | XM_017021208.1 | 99.7% | 99.3% | 578A>C;1000C>T;1316G>T |
| 4 | human | 26094 | DCAF4 | DDB1 and CUL4 associated fa... | NM_001352449.2 | 99.5% | 99.1% | (many diffs) |
| 5 | human | 26094 | DCAF4 | DDB1 and CUL4 associated fa... | XM_017021201.1 | 99.5% | 99.1% | (many diffs) |
| 6 | human | 26094 | DCAF4 | DDB1 and CUL4 associated fa... | XM_017021202.1 | 99.5% | 99.1% | (many diffs) |
| 7 | human | 26094 | DCAF4 | DDB1 and CUL4 associated fa... | XM_017021203.1 | 99.5% | 99.1% | (many diffs) |
| 8 | human | 26094 | DCAF4 | DDB1 and CUL4 associated fa... | XM_017021205.1 | 99.5% | 99.1% | (many diffs) |
| 9 | human | 26094 | DCAF4 | DDB1 and CUL4 associated fa... | XM_017021206.1 | 99.5% | 99.1% | (many diffs) |
| 10 | human | 26094 | DCAF4 | DDB1 and CUL4 associated fa... | NM_001163508.2 | 98.2% | 97.9% | (many diffs) |
| 11 | human | 26094 | DCAF4 | DDB1 and CUL4 associated fa... | XM_017021214.1 | 95.3% | 84.2% | (many diffs) |
| 12 | human | 26094 | DCAF4 | DDB1 and CUL4 associated fa... | NM_001163509.2 | 95.1% | 84% | (many diffs) |
| 13 | human | 26094 | DCAF4 | DDB1 and CUL4 associated fa... | XM_017021212.1 | 95.1% | 84% | (many diffs) |
| 14 | human | 26094 | DCAF4 | DDB1 and CUL4 associated fa... | XM_017021213.2 | 95.1% | 84% | (many diffs) |
| 15 | human | 26094 | DCAF4 | DDB1 and CUL4 associated fa... | NM_001352448.2 | 88.2% | 85.5% | (many diffs) |
| 16 | human | 26094 | DCAF4 | DDB1 and CUL4 associated fa... | XM_017021209.1 | 88% | 85.3% | (many diffs) |
| 17 | human | 26094 | DCAF4 | DDB1 and CUL4 associated fa... | NM_181341.3 | 87.2% | 86.8% | (many diffs) |
| 18 | human | 26094 | DCAF4 | DDB1 and CUL4 associated fa... | NM_181340.3 | 79.5% | 79.1% | (many diffs) |
| 19 | human | 26094 | DCAF4 | DDB1 and CUL4 associated fa... | NR_147990.2 | 63.2% | (many diffs) | |
| 20 | human | 26094 | DCAF4 | DDB1 and CUL4 associated fa... | XM_017021215.2 | 56.4% | 56.1% | 0_1ins645;355C>T;671G>T |
| 21 | human | 26094 | DCAF4 | DDB1 and CUL4 associated fa... | NM_001352447.2 | 56.3% | 56% | (many diffs) |
| 22 | mouse | 73828 | Dcaf4 | DDB1 and CUL4 associated fa... | XM_006516283.2 | 56.3% | 56.6% | (many diffs) |
| 23 | mouse | 73828 | Dcaf4 | DDB1 and CUL4 associated fa... | XM_006516284.2 | 46.9% | 47.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1551
- ORF length:
- 1485
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgaa taaaagtcgc tggcagagta gaagacgaca tgggagaaga agccaccagc 121 agaacccttg gttcagactc cgtgattctg aagacaggtc tgactcccgg gcagcacagc 181 ccgctcacga ttccggccac ggtgatgacg agtctccgtc aacctcgtct ggcacagctg 241 ggacctcctc tgtgccagag ctacctgggt tttactttga ccctgaaaag aaacgctact 301 tccgcttgct ccctggacat aacaactgca accccctgac gaaagagagc atccggcaga 361 aggagatgga gagcaagaga ctgcggctgc tccaggaaga agacagacgg aaaaagattg 421 ccaggatggg atttaatgca tcttccatgc tacgaaaaag ccagctgggt tttctcaacg 481 tcaccaatta ctgccattta gcccacgagc tgcgtctcag ctgcatggag aggaaaaagg 541 tccagattcg aagcatggat ccctccgcct tggcaagcga ccgatttaac ctcatactgg 601 cagataccaa cagtgaccgg ctcttcacag tgaacgatgt tacagttgga ggctccaagt 661 atggtatcat caacctgcaa agtctgaaga cccctacgct caaggtgttc atgcacgaaa 721 acctctactt caccaaccgg aaggtgaatt cggtgtgctg ggcctcgctg aatcacttgg 781 attcccacat tctgctatgc ctcatgggac tcgcagagac tccaggctgt gccaccctgc 841 tcccagcatc actgttcgtc aatagtcacc caggaataga ccggcctggc atgctctgca 901 gtttccggat ccctggtgcc tggtcctgtg cctggtccct gaatatccaa gcaaataact 961 gcttcagtac aggcttgtct cggcgggtcc tgttgaccaa cgtggtgacg ggacaccggc 1021 agtcctttgg gaccaacagt gatgtcttgg cccagcagtt tgctttcatg gctcctctgc 1081 tgtttaatgg ctgccgctct ggggaaatct ttgccattga tctgcgttgt ggaaatcaag 1141 gcaagggatg gaaggccacc cgcctgtttc atgattcagc agtgacctct gtgcggatcc 1201 tccaagatga gcaatacctg atggcttcag acatggctgg aaagatcaag ctgtgggacc 1261 tgaggaccac gaagtgcgta aggcagtacg aaggccacgt gaatgagtac gcctacctgc 1321 ccctgcatgt gcacgaggaa gaaggaatcc tggtggcagt gggccaggac tgctacacga 1381 taatctggag cctccacgat gcccgcctac tgagaaccat accctccccg taccctgcct 1441 ccaaggccga cattcccagt gtggccttct cgtcgcggct ggggggctcc cggggcgcgc 1501 cggggctgct catggctgtc gggcaggacc tttactgtta ctcctacagc tacccaactt 1561 tcttgtacaa agtggttggt aagcctatcc ctaaccctct cctcggtctc gattctacgt 1621 agtaatgagc tagcgctaac cggtggcgcg ttaagtcgac aatcaacctc tggatta