Construct: ORF ccsbBroad304_08004
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013511.1_s304c1, BRDN0000406922
- Derived from:
- ccsbBroadEn_08004
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FBXL5 (26234)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_08004
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 26234 | FBXL5 | F-box and leucine rich repe... | NM_012161.4 | 99.8% | 99.7% | 155A>C;312A>G;724G>A |
| 2 | human | 26234 | FBXL5 | F-box and leucine rich repe... | NM_001193534.1 | 99.7% | 99.5% | (many diffs) |
| 3 | human | 26234 | FBXL5 | F-box and leucine rich repe... | NM_001193535.1 | 97.3% | 97.2% | (many diffs) |
| 4 | human | 26234 | FBXL5 | F-box and leucine rich repe... | XM_006713959.3 | 96.7% | 94.9% | (many diffs) |
| 5 | human | 26234 | FBXL5 | F-box and leucine rich repe... | XM_011513831.2 | 94.4% | 93.1% | (many diffs) |
| 6 | human | 26234 | FBXL5 | F-box and leucine rich repe... | XM_011513832.2 | 91.9% | 91.8% | 0_1ins165;147A>G;559G>A |
| 7 | human | 26234 | FBXL5 | F-box and leucine rich repe... | XM_017008019.2 | 91.9% | 91.8% | 0_1ins165;147A>G;559G>A |
| 8 | human | 26234 | FBXL5 | F-box and leucine rich repe... | XM_011513833.2 | 89.4% | 89.4% | 81_82ins216;96A>G;508G>A |
| 9 | human | 26234 | FBXL5 | F-box and leucine rich repe... | NR_036464.1 | 56.8% | (many diffs) | |
| 10 | mouse | 242960 | Fbxl5 | F-box and leucine-rich repe... | NM_001159963.1 | 90.4% | 93.3% | (many diffs) |
| 11 | mouse | 242960 | Fbxl5 | F-box and leucine-rich repe... | NM_178729.4 | 81% | 82.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2139
- ORF length:
- 2073
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggc gccctttcct gaagaagtgg acgtcttcac cgccccacac tggcggatga 121 agcagctggt ggggctctac tgcgacaagc tttctaaaac caatttttcc aacaacaacg 181 atttccgtgc tcttctgcag tctttgtatg ctactttcac ggagttcaaa atgcatgagc 241 agattgaaaa tgaatacatt attggtttgc ttcaacaacg cagccagacc atttataatg 301 tacattctga caataaactc tccgagatgc ttagcctctt tgaaaaggga ctgaagaatg 361 ttaagaatga atatgagcag ttaaattatg caaaacaact gaaagagaga ttggaggctt 421 ttacaagaga ttttcttcct cacatgaaag aggaagagga ggtttttcag cccatgttaa 481 tggaatattt tacctatgaa gagcttaagg atattaaaaa gaaagtgatt gcacaacact 541 gctctcagaa ggatactgca gaactcctta gaggtcttag cctatggaat catgctgaag 601 agcgacagaa gttttttaaa tattccgtgg atgaaaagtc agataaagaa gcagaagtgt 661 cagaacactc cacaggtata acccatcttc ctcctgaggt aatgctgtca attttcagct 721 atcttaatcc tcaagagtta tgtcgatgca gtcaagtaag catgaaatgg tctcagctga 781 caaaaacgag atcgctttgg aaacatcttt accctgttca ttgggccaga ggtgactggt 841 atagtggtcc cgcaactgaa cttgatactg aacctgatga tgaatgggtg aaaaatagga 901 aagatgaaag tcgtgctttt catgagtggg atgaagatgc tgacattgat gaatctgaag 961 agtctgcgga ggaatcaatt gctatcagca ttgcacaaat ggaaaaacgt ttactccatg 1021 gcttaattca taacgttcta ccatatgttg gtacttctgt aaaaacctta gtattagcat 1081 acagctctgc agtttccagc aaaatggtta ggcagatttt agagctttgt cctaacctgg 1141 agcatctgga tcttacccag actgacattt cagattctgc atttgacagt tggtcttggc 1201 ttggttgctg ccagagtctt cggcatcttg atctgtctgg ttgtgagaaa atcacagatg 1261 tggccctaga gaagatttcc agagctcttg gaattctgac atctcatcaa agtggctttt 1321 tgaaaacatc tacaagcaaa attacttcaa ctgcgtggaa aaataaagac attaccatgc 1381 agtccaccaa gcagtatgcc tgtttgcacg atttaactaa caagggcatt ggagaagaaa 1441 tagataatga acacccctgg actaagcctg tttcttctga gaatttcact tctccttatg 1501 tgtggatgtt agatgctgaa gatttggctg atattgaaga tactgtggaa tggagacata 1561 gaaatgttga aagtctttgt gtaatggaaa cagcatccaa ctttagttgt tccacctctg 1621 gttgttttag taaggacatt gttggactaa ggactagtgt ctgttggcag cagcattgtg 1681 cttctccagc ctttgcgtat tgtggtcact cattttgttg tacaggaaca gctttaagaa 1741 ctatgtcatc actcccagaa tcttctgcaa tgtgtagaaa agcagcaagg actagattgc 1801 ctaggggaaa agacttaatt tactttggga gtgaaaaatc tgatcaagag actggacgtg 1861 tacttctgtt tctcagttta tctggatgtt atcagatcac agaccatggt ctcagggttt 1921 tgactctggg aggagggctg ccttatttgg agcaccttaa tctctctggt tgtcttacta 1981 taactggtgc aggcctgcag gatttggttt cagcatgtcc ttctctgaat gatgaatact 2041 tttactactg tgacaacatt aacggtcctc atgctgatac cgccagtgga tgccagaatt 2101 tgcagtgtgg ttttcgagcc tgctgccgct ctggcgaatg cccaactttc ttgtacaaag 2161 tggttggtaa gcctatccct aaccctctcc tcggtctcga ttctacgtag taatgagcta 2221 gcgctaaccg gtggcgcgtt aagtcgacaa tcaacctctg gatta