Construct: ORF ccsbBroad304_08050
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001518.1_s304c1, BRDN0000408552
- Derived from:
- ccsbBroadEn_08050
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- STAU2 (27067)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_08050
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 27067 | STAU2 | staufen double-stranded RNA... | NM_001164384.1 | 99.9% | 99.7% | 496G>A |
| 2 | human | 27067 | STAU2 | staufen double-stranded RNA... | NM_014393.2 | 99.9% | 99.7% | 496G>A |
| 3 | human | 27067 | STAU2 | staufen double-stranded RNA... | NM_001164385.1 | 98.6% | 98.5% | 0_1insATGCTTCAAATAAATCAG;478G>A |
| 4 | human | 27067 | STAU2 | staufen double-stranded RNA... | NM_001164381.1 | 88.9% | 88.6% | 496G>A;1436C>T;1438_1614del |
| 5 | human | 27067 | STAU2 | staufen double-stranded RNA... | NM_001164380.2 | 83.1% | 82.8% | (many diffs) |
| 6 | human | 27067 | STAU2 | staufen double-stranded RNA... | NM_001164382.1 | 70.5% | 70.4% | 0_1ins216;280G>A;1221_1512delinsA |
| 7 | human | 27067 | STAU2 | staufen double-stranded RNA... | NM_001164383.1 | 62.8% | 62.6% | (many diffs) |
| 8 | mouse | 29819 | Stau2 | staufen (RNA binding protei... | NM_001347045.1 | 91.3% | 96% | (many diffs) |
| 9 | mouse | 29819 | Stau2 | staufen (RNA binding protei... | XM_017321109.1 | 89.2% | 93.8% | (many diffs) |
| 10 | mouse | 29819 | Stau2 | staufen (RNA binding protei... | NM_001347044.1 | 85.3% | 89% | (many diffs) |
| 11 | mouse | 29819 | Stau2 | staufen (RNA binding protei... | XM_011238390.2 | 83.6% | 87.1% | (many diffs) |
| 12 | mouse | 29819 | Stau2 | staufen (RNA binding protei... | NM_001111272.1 | 81.2% | 85.3% | (many diffs) |
| 13 | mouse | 29819 | Stau2 | staufen (RNA binding protei... | XM_006495532.3 | 81.2% | 85.3% | (many diffs) |
| 14 | mouse | 29819 | Stau2 | staufen (RNA binding protei... | XM_011238389.1 | 81.2% | 85.3% | (many diffs) |
| 15 | mouse | 29819 | Stau2 | staufen (RNA binding protei... | XM_011238388.2 | 79.7% | 83.1% | (many diffs) |
| 16 | mouse | 29819 | Stau2 | staufen (RNA binding protei... | NM_025303.3 | 76.4% | 79.6% | (many diffs) |
| 17 | mouse | 29819 | Stau2 | staufen (RNA binding protei... | XM_006495528.3 | 76.4% | 79.6% | (many diffs) |
| 18 | mouse | 29819 | Stau2 | staufen (RNA binding protei... | XM_011238387.1 | 76.4% | 79.6% | (many diffs) |
| 19 | mouse | 29819 | Stau2 | staufen (RNA binding protei... | XM_017321102.1 | 76.4% | 79.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1506
- ORF length:
- 1437
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat gcttcaaata aatcagatgt tctcagtgca gctgagtctt ggtgagcaga 121 catgggaatc cgaaggcagc agtataaaga aggctcagca ggctgttgcc aataaagctt 181 tgactgaatc tacgcttccc aaaccagttc agaagccacc caaaagtaat gttaacaata 241 acccaggcag tataactcca actgtggaac tgaatgggct tgctatgaaa aggggagagc 301 ctgccatcta caggccatta gatccaaagc cattcccaaa ttatagagct aattacaact 361 ttcggggcat gtacaatcag aggtatcatt gcccagtgcc taagatcttt tatgttcagc 421 tcactgtagg aaataatgaa ttttttgggg aaggaaagac tcgacaagct gctagacaca 481 atgctgcaat gaaagccctc caagcactgc agaatgaacc tattccagaa agatctcctc 541 agaatggtga atcaggaaag gatatggatg atgacaaaga tgcaaataag tctgagatca 601 gcttagtgtt tgaaattgct ctgaagcgaa atatgcctgt cagttttgag gttattaaag 661 aaagtggacc accacatatg aaaagctttg ttactcgagt gtcagtagga gagttctctg 721 cagaaggaga aggaaatagc aaaaaactct ccaagaagcg cgctgcgacc accgtcttac 781 aggagcttaa aaaacttcca cctcttcctg tggtggaaaa gccaaaacta ttttttaaaa 841 aacgccctaa aacaatagta aaggccggac cagaatatgg ccaagggatg aaccctatta 901 gccgcctggc gcaaattcaa caggccaaaa aggaaaagga gccggattat gttttgcttt 961 cagaaagagg aatgcctcga cgtcgagaat ttgtgatgca ggtgaaggta ggcaatgaag 1021 ttgctacagg aacaggacct aataaaaaga tagccaaaaa aaatgctgca gaagcaatgc 1081 tgttacaact tggttataaa gcatccacta atcttcagga tcaacttgag aagacagggg 1141 aaaacaaagg atggagtggt ccaaagcctg ggtttcctga accaacaaat aatactccaa 1201 aaggaattct tcatttgtct cctgatgttt atcaagagat ggaagccagc cgccacaaag 1261 taatctctgg cactactcta ggctatttgt cacccaaaga tatgaaccaa ccttcaagct 1321 ctttcttcag tatatctccc acatcgaata gttcagctac aattgccagg gaactcctta 1381 tgaatggaac atcttctaca gctgaagcca taggtttaaa aggaagttct cctactcccc 1441 cttgttctcc agtacaacct tcaaaacaac tggaatattt agcaaggatt caaggctttc 1501 aggtattgcc aactttcttg tacaaagtgg ttggtaagcc tatccctaac cctctcctcg 1561 gtctcgattc tacgtagtaa tgagctagcg ctaaccggtg gcgcgttaag tcgacaatca 1621 acctctggat ta