Construct: ORF ccsbBroad304_08062
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009828.1_s304c1, BRDN0000462189
- Derived from:
- ccsbBroadEn_08062
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PALD1 (27143)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_08062
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 27143 | PALD1 | phosphatase domain containi... | NM_014431.3 | 99.8% | 99.8% | 156C>A;422C>T;2286A>G |
| 2 | human | 27143 | PALD1 | phosphatase domain containi... | XM_011539637.2 | 99.8% | 99.8% | 156C>A;422C>T;2286A>G |
| 3 | human | 27143 | PALD1 | phosphatase domain containi... | XM_011539638.2 | 99.8% | 99.8% | 156C>A;422C>T;2286A>G |
| 4 | human | 27143 | PALD1 | phosphatase domain containi... | XM_017016072.1 | 99.8% | 99.8% | 156C>A;422C>T;2286A>G |
| 5 | human | 27143 | PALD1 | phosphatase domain containi... | XR_945675.2 | 55% | (many diffs) | |
| 6 | human | 27143 | PALD1 | phosphatase domain containi... | XR_001747093.1 | 47.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2637
- ORF length:
- 2568
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat gggtacaacg gccagcacag cccagcagac ggtctcggca ggcaccccat 121 ttgagggcct acagggcagt ggcacgatgg acagtcggca ctccgtcagc atccactcct 181 tccagagcac tagcttgcat aacagcaagg ccaagtccat catacccaac aaggtggccc 241 ctgttgtgat cacgtacaac tgcaaggagg agttccagat ccatgatgag ctgctcaagg 301 ctcattacac gttgggccgg ctctcggaca acacccctga gcactacctg gtgcaaggcc 361 gctacttcct ggtgcgggat gtcactgaga agatggatgt gctgggcacc gtgggaagct 421 gtggggcccc caacttccgg caggtgcagg gtgggctcac tgtgttcggc atgggacagc 481 ccagcctctt agggttcagg cgggtcctcc agaaactcca gaaggacgga catagggagt 541 gtgtcatctt ctgtgtgcgg gaggaacctg tgcttttcct gcgtgcagat gaggactttg 601 tgtcctacac acctcgagac aagcagaacc ttcatgagaa cctccagggc cttggacccg 661 gggtccgggt ggagagcctg gagctggcca tccggaaaga gatccacgac tttgcccagc 721 tgagcgagaa cacataccat gtgtaccata acaccgagga cctgtggggg gagccccatg 781 ctgtggccat ccatggtgag gacgacttgc atgtgacgga ggaggtgtac aagcggcccc 841 tcttcctgca gcccacctac aggtaccacc gcctgcccct gcccgagcaa gggagtcccc 901 tggaggccca gttggacgcc tttgtcagtg ttctccggga gacccccagc ctgctgcagc 961 tccgtgatgc ccacgggcct cccccagccc tcgtcttcag ctgccagatg ggcgtgggca 1021 ggaccaacct gggcatggtc ctgggcaccc tcatcctgct tcaccgcagt gggaccacct 1081 cccagccaga ggctgccccc acgcaggcca agcccctgcc tatggagcag ttccaggtga 1141 tccagagctt tctccgcatg gtgccccagg gaaggaggat ggtggaagag gtggacagag 1201 ccatcactgc ctgtgccgag ttgcatgacc tgaaagaagt ggtcttggaa aaccagaaga 1261 agttagaagg tatccgaccg gagagcccag cccagggaag cggcagccga cacagcgtct 1321 ggcagagggc gctgtggagc ctggagcgat acttctacct gatcctgttt aactactacc 1381 ttcatgagca gtacccgctg gcctttgccc tcagtttcag ccgctggctg tgtgcccacc 1441 ctgagctgta ccgcctgccc gtgacgctga gctcagcagg ccctgtggct ccgagggacc 1501 tcatcgccag gggctcccta cgggaggacg atctggtctc cccggacgcg ctcagcactg 1561 tcagagagat ggatgtggcc aacttccggc gggtgccccg catgcccatc tacggcacgg 1621 cccagcccag cgccaaggcc ctggggagca tcctggccta cctgacggac gccaagagga 1681 ggctgcggaa ggttgtctgg gtgagccttc gggaggaggc cgtgttggag tgtgacgggc 1741 acacctacag cctgcggtgg cctgggcccc ctgtggctcc tgaccagctg gagaccctgg 1801 aggcccagct gaaggcccat ctaagcgagc ctcccccagg caaggagggc cccctgacct 1861 acaggttcca gacctgcctt accatgcagg aggtcttcag ccagcaccgc agggcctgtc 1921 ctggcctcac ctaccaccgc atccccatgc cggacttctg tgccccccga gaggaggact 1981 ttgaccagct gctggaggcc ctgcgggccg ccctctccaa ggacccaggc actggcttcg 2041 tgttcagctg cctcagcggc cagggccgta ccacaactgc gatggtggtg gctgtcctgg 2101 ccttctggca catccaaggc ttccccgagg tgggtgagga ggagctcgtg agtgtgcctg 2161 atgccaagtt cactaagggt gaatttcagg tagtaatgaa ggtggtgcag ctgctacccg 2221 atgggcaccg tgtgaagaag gaggtggacg cagcgctgga cactgtcagc gagaccatga 2281 cgcccatgca ctaccacctg cgggagatca tcatctgcac ctaccgccag gcgaaggcag 2341 cgaaagaggc gcaggaaatg cggaggctgc agctgcggag cctgcagtac ttggagcgct 2401 atgtctgcct gattctcttc aacgcgtacc tccacctgga gaaggccgac tcctggcaga 2461 ggcccttcag cacctggatg caggaggtgg catcgaaggc tggcatctac gagatcctta 2521 acgagctggg cttccccgag ctggagagcg gggaggacca gcccttctcc aggctgcgct 2581 accggtggca ggagcagagc tgcagcctcg agccctctgc ccccgaggac ttgctgttgc 2641 caactttctt gtacaaagtg gttggtaagc ctatccctaa ccctctcctc ggtctcgatt 2701 ctacgtagta atgagctagc gctaaccggt ggcgcgttaa gtcgacaatc aacctctgga 2761 tta