Transcript: Human XR_945675.2

PREDICTED: Homo sapiens phosphatase domain containing paladin 1 (PALD1), transcript variant X5, misc_RNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
PALD1 (27143)
Length:
4595
CDS:
(non-coding)

Additional Resources:

NCBI RefSeq record:
XR_945675.2
NBCI Gene record:
PALD1 (27143)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the exonic sequence of this non-coding transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XR_945675.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000030020 CCCTGTTGTGATCACGTACAA pLKO.1 501 3UTR 100% 4.950 3.960 N Pald1 n/a
2 TRCN0000264147 TGTTCTGGATAGATCTATTAT pLKO_005 3400 3UTR 100% 15.000 10.500 N PALD1 n/a
3 TRCN0000264144 AGCGAAAGAGGCGCAAGAAAT pLKO_005 2578 3UTR 100% 13.200 9.240 N PALD1 n/a
4 TRCN0000264145 ACATACCATGTGTACCATAAC pLKO_005 994 3UTR 100% 10.800 7.560 N PALD1 n/a
5 TRCN0000264146 GAGCTGCTCAAGGCTCATTAC pLKO_005 550 3UTR 100% 10.800 7.560 N PALD1 n/a
6 TRCN0000282929 GGTGAATTTCAGGTAGTAATG pLKO_005 2417 3UTR 100% 10.800 7.560 N PALD1 n/a
7 TRCN0000182987 CCAGAAGAAGTTAGAAGGTAT pLKO.1 1515 3UTR 100% 4.950 3.465 N PALD1 n/a
8 TRCN0000183773 CCTGATCCTGTTTAACTACTA pLKO.1 1620 3UTR 100% 4.950 3.465 N PALD1 n/a
9 TRCN0000146731 CCCATTTATCCAGTATTGGAA pLKO.1 4206 3UTR 100% 3.000 2.100 N PALD1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XR_945675.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_08062 pDONR223 100% 55% None (many diffs) n/a
2 ccsbBroad304_08062 pLX_304 0% 55% V5 (many diffs) n/a
3 TRCN0000476462 CCTCCGTTCTGATTTCAAAAGTCC pLX_317 13.4% 55% V5 (many diffs) n/a
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