Construct: ORF ccsbBroad304_08088
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016258.1_s304c1, BRDN0000399084
- Derived from:
- ccsbBroadEn_08088
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- HTRA2 (27429)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_08088
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 27429 | HTRA2 | HtrA serine peptidase 2 | NM_013247.4 | 99.9% | 99.7% | 6T>N |
| 2 | human | 27429 | HTRA2 | HtrA serine peptidase 2 | NM_001321728.1 | 92.9% | 92.7% | 6T>N;1114_1115ins96 |
| 3 | human | 27429 | HTRA2 | HtrA serine peptidase 2 | NM_001321727.1 | 90.9% | 90.8% | 6T>N;939_968del;1144_1145ins96 |
| 4 | human | 27429 | HTRA2 | HtrA serine peptidase 2 | NM_145074.2 | 78.7% | 78.6% | 6T>N;711_712ins195;919_920ins96 |
| 5 | human | 27429 | HTRA2 | HtrA serine peptidase 2 | NR_135770.1 | 55.8% | (many diffs) | |
| 6 | human | 27429 | HTRA2 | HtrA serine peptidase 2 | NR_135772.1 | 55.6% | (many diffs) | |
| 7 | human | 27429 | HTRA2 | HtrA serine peptidase 2 | NR_135771.1 | 55.2% | (many diffs) | |
| 8 | human | 27429 | HTRA2 | HtrA serine peptidase 2 | NR_135769.1 | 53% | (many diffs) | |
| 9 | mouse | 64704 | Htra2 | HtrA serine peptidase 2 | NM_019752.3 | 86.4% | 84.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1440
- ORF length:
- 1374
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggc ngcgccgagg gcggggcggg gtgcaggctg gagccttcgg gcatggcggg 121 ctttgggggg cattcgctgg gggaggagac cccgtttgac ccctgacctc cgggccctgc 181 tgacgtcagg aacttctgac ccccgggccc gagtgactta tgggaccccc agtctctggg 241 cccggttgtc tgttggggtc actgaacccc gagcatgcct gacgtctggg accccgggtc 301 cccgggcaca actgactgcg gtgaccccag ataccaggac ccgggaggcc tcagagaact 361 ctggaacccg ttcgcgcgcg tggctggcgg tggcgctggg cgctgggggg gcagtgctgt 421 tgttgttgtg gggcgggggt cggggtcctc cggccgtcct cgccgccgtc cctagcccgc 481 cgcccgcttc tccccggagt cagtacaact tcatcgcaga tgtggtggag aagacagcac 541 ctgccgtggt ctatatcgag atcctggacc ggcacccttt cttgggccgc gaggtcccta 601 tctcgaacgg ctcaggattc gtggtggctg ccgatgggct cattgtcacc aacgcccatg 661 tggtggctga tcggcgcaga gtccgtgtga gactgctaag cggcgacacg tatgaggccg 721 tggtcacagc tgtggatccc gtggcagaca tcgcaacgct gaggattcag actaaggagc 781 ctctccccac gctgcctctg ggacgctcag ctgatgtccg gcaaggggag tttgttgttg 841 ccatgggaag tccctttgca ctgcagaaca cgatcacatc cggcattgtt agctctgctc 901 agcgtccagc cagagacctg ggactccccc aaaccaatgt ggaatacatt caaactgatg 961 cagctattga ttttggaaac tctggaggtc ccctggttaa cctggatggg gaggtgattg 1021 gagtgaacac catgaaggtc acagctggaa tctcctttgc catcccttct gatcgtcttc 1081 gagagtttct gcatcgtggg gaaaagaaga attcctcctc cggaatcagt gggtcccagc 1141 ggcgctacat tggggtgatg atgctgaccc tgagtcccag catccttgct gaactacagc 1201 ttcgagaacc aagctttccc gatgttcagc atggtgtact catccataaa gtcatcctgg 1261 gctcccctgc acaccgggct ggtctgcggc ctggtgatgt gattttggcc attggggagc 1321 agatggtaca aaatgctgaa gatgtttatg aagctgttcg aacccaatcc cagttggcag 1381 tgcagatccg gcggggacga gaaacactga ccttatatgt gacccctgag gtcacagaat 1441 gcccaacttt cttgtacaaa gtggttggta agcctatccc taaccctctc ctcggtctcg 1501 attctacgta gtaatgagct agcgctaacc ggtggcgcgt taagtcgaca atcaacctct 1561 ggatta