Transcript: Human NM_013247.4

Homo sapiens HtrA serine peptidase 2 (HTRA2), transcript variant 1, mRNA.

Source:
NCBI, updated 2019-07-29
Taxon:
Homo sapiens (human)
Gene:
HTRA2 (27429)
Length:
2550
CDS:
603..1979

Additional Resources:

NCBI RefSeq record:
NM_013247.4
NBCI Gene record:
HTRA2 (27429)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_013247.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000353041 CATGGTGTACTCATCCATAAA pLKO_005 1767 CDS 100% 13.200 18.480 N HTRA2 n/a
2 TRCN0000344115 ACGCTGAGGATTCAGACTAAG pLKO_005 1293 CDS 100% 10.800 15.120 N HTRA2 n/a
3 TRCN0000344116 GATCACATCCGGCATTGTTAG pLKO_005 1409 CDS 100% 10.800 15.120 N HTRA2 n/a
4 TRCN0000075206 CCAAACCAATGTGGAATACAT pLKO.1 1466 CDS 100% 5.625 7.875 N HTRA2 n/a
5 TRCN0000075204 CCGGAGTCAGTACAACTTCAT pLKO.1 1031 CDS 100% 4.950 6.930 N HTRA2 n/a
6 TRCN0000344171 AGCACCTGCCGTGGTCTATAT pLKO_005 1073 CDS 100% 13.200 10.560 N HTRA2 n/a
7 TRCN0000352977 CTGATCGTCTTCGAGAGTTTC pLKO_005 1606 CDS 100% 10.800 8.640 N HTRA2 n/a
8 TRCN0000075207 CCCAAACCAATGTGGAATACA pLKO.1 1465 CDS 100% 5.625 4.500 N HTRA2 n/a
9 TRCN0000075203 GAAGAATCACAGAAACACTTT pLKO.1 2121 3UTR 100% 4.950 3.465 N HTRA2 n/a
10 TRCN0000075205 GCTGAACTACAGCTTCGAGAA pLKO.1 1725 CDS 100% 4.050 2.835 N HTRA2 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_013247.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_08088 pDONR223 98.5% 99.9% 99.7% None 6T>N n/a
2 ccsbBroad304_08088 pLX_304 0% 99.9% 99.7% V5 6T>N n/a
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