Construct: ORF ccsbBroad304_08115

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF013823.1_s304c1, BRDN0000461741
Derived from:
ccsbBroadEn_08115
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
FHOD1 (29109)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_08115
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 29109 FHOD1 formin homology 2 domain co... NM_013241.3 99.9% 100% 2478G>T
2 human 29109 FHOD1 formin homology 2 domain co... NM_001318202.2 97.7% 97.8% 1319_1396del;2556G>T
3 human 29109 FHOD1 formin homology 2 domain co... XM_011523044.1 93.2% 93.2% 1319_1396del;2556G>T;2581_2582ins162
4 human 29109 FHOD1 formin homology 2 domain co... XM_011523043.2 93.1% 92.5% (many diffs)
5 human 29109 FHOD1 formin homology 2 domain co... XR_001751895.1 90.1% (many diffs)
6 human 29109 FHOD1 formin homology 2 domain co... XM_011523045.2 89.9% 90% 0_1ins279;1040_1117del;2277G>T
7 human 29109 FHOD1 formin homology 2 domain co... XR_933285.1 89.7% (many diffs)
8 human 29109 FHOD1 formin homology 2 domain co... XR_933286.1 78.7% (many diffs)
9 human 29109 FHOD1 formin homology 2 domain co... XM_006721180.1 59.6% 59.7% 0_1ins1407;1071G>T
10 mouse 234686 Fhod1 formin homology 2 domain co... NM_177699.4 82.7% 85.8% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
3558
ORF length:
3492
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatggc gggcggggaa gaccgcgggg acggagagcc ggtatcagtg gtgaccgtga
121gggtgcagta cctggaagac accgacccct tcgcatgtgc caactttccg gagccgcgcc
181gggcccccac ctgcagcctg gacggggcgc tgcccttggg cgcgcagata cccgcggtgc
241accgcctgct gggagcgccg ctcaagttgg aggattgtgc tctgcaagtg tctccctccg
301gatactacct ggacaccgag ctgtccctgg aagagcagcg ggagatgctg gagggcttct
361atgaagagat cagcaaaggg cggaagccca cgctgatcct tcggacccag ctctctgtga
421gggtcaacgc tatcttggaa aagctgtata gctccagtgg tcctgagctc cgccgctccc
481tcttctcact gaagcagatc ttccaggagg acaaagacct ggtgcctgaa tttgtgcatt
541cagaggggct gagctgcctg atccgtgtgg gtgctgctgc cgaccacaac taccagagct
601acatccttag agcgctcggc cagctgatgc tctttgtgga tggaatgctg ggggtggtgg
661cccacagtga cactattcag tggctgtaca cattgtgtgc cagcctgtcc cgcttggtgg
721tgaagacagc cctgaagctg ctgttggtgt ttgtagaata ctccgaaaac aacgcaccgc
781tgttcatccg tgcagtgaac tctgtggcca gcaccaccgg tgctcctccc tgggccaatc
841tggtgtccat cctggaggag aagaatggcg ctgaccctga gttgttggtg tacacggtca
901ccctcatcaa caagacgctg gcggcgctcc cggaccagga ctccttctac gatgtgacgg
961atgcactgga gcagcagggc atggaagcgc tggtccagcg ccacctgggc actgcgggca
1021ctgacgtcga cctgcgcacg cagcttgtgc tctacgagaa cgccctgaaa ttggaggatg
1081gagacatcga agaagcccca ggcgctggtg ggcggcggga acgacgaaag ccttcttctg
1141aggagggcaa gaggagccgc cgttctctgg aaggcggggg ctgccccgcg cgtgccccgg
1201aacctggccc cacaggcccc gcctcaccgg taggccccac ctcttccacc ggccccgccc
1261tgctgacagg ccccgcctcc agccctgtgg gccctccctc cggtctccaa gcttcagtga
1321acctttttcc taccatctct gtggcaccct cagctgacac ctccagcgag aggagcatct
1381acaaagcccg gttcctggag aatgtggcgg cagcagaaac agagaagcag gttgcgctgg
1441cccagggccg ggcagagaca cttgccgggg ccatgcccaa tgaggcgggt ggacacccag
1501atgcccggca actctgggac tccccagaga cagcccctgc agccagaaca ccccagagcc
1561ctgccccctg tgtcctgctc cgggcccagc gaagccttgc accagagccc aaggagccac
1621tgataccagc aagccccaag gctgagccca tctgggagct ccctacccgt gcacccaggc
1681tctctattgg ggacctggac ttttcagatc taggggagga tgaagaccag gacatgctga
1741atgtagagtc tgtggaggct gggaaagaca tcccagctcc ctcaccccca ctgcccctgc
1801tctcgggagt acccccccct cccccacttc cacctccccc acccatcaaa ggccccttcc
1861caccacctcc acctctacct ctggctgccc ctcttcccca ttcagtgcct gacagctcag
1921ccctccccac taagaggaag acagtaaaac ttttctggcg tgagctgaag ctggctgggg
1981gccatggagt ctctgcaagc cgctttgggc cctgcgccac cctctgggct tcactggacc
2041ctgtctcagt ggacacggcc cgactggaac acctctttga gtctcgtgcc aaagaggtgc
2101tgccctccaa gaaagctgga gagggccgcc ggacaatgac cacagtgctg gaccccaagc
2161gcagcaacgc catcaacatc ggcctaacca cactgccacc tgtgcatgtc attaaggctg
2221ctctgctcaa ctttgatgag tttgctgtca gcaaggatgg cattgagaag ctactgacca
2281tgatgcccac ggaggaagag cggcagaaga ttgaggaagc ccagctggcc aaccctgaca
2341tacccctggg cccagccgag aacttcctga tgactcttgc ctccattggc ggcctcgctg
2401ctcgtctaca actctgggcc ttcaagctgg actatgacag catggagcgg gaaattgctg
2461agccactgtt tgacctgaaa gtgggtatgg aacagctggt acagaatgcc accttccgct
2521gcatcctggc taccctccta gctgtgggca acttcctcaa tggctcccag agcagcggct
2581ttgagctgag ctacctggag aaggtgtcag aggtgaagga cacggtgcgt cgacagtcac
2641tgctacacca tctctgctcc ctagtgctcc agacccggcc tgagtcctct gacctctatt
2701cagaaatccc tgccctgacc cgctgtgcca aggtggactt tgaacagctg actgagaacc
2761tggggcagct ggagcgccgg agccgggcag ccgaggagag cctgcggagc ttggccaagc
2821atgagctggc cccagccctg cgtgcccgcc tcacccactt cctggaccag tgtgcccgcc
2881gtgttgccat gctaaggata gtgcaccgcc gtgtctgcaa taggttccat gccttcctgc
2941tctacctggg ctacaccccg caggcggccc gtgaagtgcg catcatgcag ttctgccaca
3001cgctgcggga atttgcgctt gagtatcgga cttgccggga acgagtgcta cagcagcagc
3061agaagcaggc cacataccgt gagcgcaaca agacccgggg acgcatgatc accgagacag
3121agaagttctc aggtgtggct ggggaagccc ccagcaaccc ctctgtccca gtagcagtga
3181gcagcgggcc aggccgggga gatgctgaca gtcatgctag tatgaagagt ctgctgacca
3241gcaggcctga ggacaccaca cacaatcgcc gcagcagagg catggtccag agcagctccc
3301caatcatgcc cacagtgggg ccctccactg catccccaga agaaccccca ggctccagtt
3361tacccagtga tacatcagat gagatcatgg accttctggt gcagtcagtg accaagagca
3421gtcctcgtgc cttagctgct agggaacgca agcgttcccg cggcaaccgc aagtctttga
3481gaaggacgtt gaagagtggg ctcggagatg acctggtgca ggcactggga ctaagcaagg
3541gtcctggcct ggaggtgtgc ccaactttct tgtacaaagt ggttggtaag cctatcccta
3601accctctcct cggtctcgat tctacgtagt aatgagctag cgctaaccgg tggcgcgtta
3661agtcgacaat caacctctgg atta

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