Construct: ORF ccsbBroad304_08132
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001696.1_s304c1, BRDN0000400305
- Derived from:
- ccsbBroadEn_08132
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GNL2 (29889)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_08132
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 29889 | GNL2 | G protein nucleolar 2 | NM_013285.3 | 99.9% | 99.8% | 851G>T |
| 2 | human | 29889 | GNL2 | G protein nucleolar 2 | NM_001323623.1 | 91.4% | 91.3% | 851G>T;910_1113del |
| 3 | human | 29889 | GNL2 | G protein nucleolar 2 | NM_001323624.1 | 74.5% | 74% | (many diffs) |
| 4 | human | 29889 | GNL2 | G protein nucleolar 2 | XM_024446591.1 | 71.5% | 71.4% | 0_1ins477;374G>T;433_636del |
| 5 | mouse | 230737 | Gnl2 | guanine nucleotide binding ... | NM_145552.2 | 84.6% | 86% | (many diffs) |
| 6 | mouse | 230737 | Gnl2 | guanine nucleotide binding ... | XM_006503029.3 | 65.3% | 65.5% | (many diffs) |
| 7 | mouse | 230737 | Gnl2 | guanine nucleotide binding ... | XM_017320153.1 | 59% | 58.6% | (many diffs) |
| 8 | mouse | 230737 | Gnl2 | guanine nucleotide binding ... | XR_001784130.1 | 56.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2259
- ORF length:
- 2193
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggt gaagcccaag tacaaaggac ggagcaccat caacccgtcc aaggccagca 121 caaacccaga tcgagtgcag ggagcaggag gccaaaacat gagggaccgg gccaccatcc 181 ggcgcctgaa tatgtatagg caaaaggagc gcaggaacag tcgtggtaaa ataattaaac 241 ccctgcaata tcaatcaacg gtggcttctg gcacagtggc aagagtagag ccaaatatta 301 aatggtttgg aaacacacgt gtgattaagc agtcatcatt acaaaaattt caagaggaaa 361 tggatacagt tatgaaggat ccatacaaag ttgtcatgaa gcaaagcaag ttaccaatgt 421 ctcttctcca tgatcgaatc cggcctcata acttgaaggt gcacattctt gatactgaaa 481 gttttgaaac tacatttggc cctaagtcac agaggaaacg accaaactta tttgcaagtg 541 atatgcagtc tcttatcgaa aatgctgaaa tgtccactga gagctatgac cagggcaagg 601 atcgtgattt ggtaactgaa gacactggtg tgagaaatga agctcaagaa gagatctata 661 aaaagggaca gtccaaaaga atatggggtg agctctacaa ggtgatagat tcatcagatg 721 ttgtagttca agttcttgat gctagagatc caatgggtac tcgttcccct cacattgaaa 781 cttacctgaa gaaggaaaaa ccttggaaac acctcatttt tgtacttaac aaatgtgacc 841 ttgttccaac ctgggcaaca aaacggtggg ttgctgtcct ctcccaggat tatccaacac 901 ttgctttcca tgcaatcctt actaacccgt ttggcaaggg agcattcatt cagcttctgc 961 ggcagtttgg aaagttgcac actgacaaga aacagatcag tgttgggttc attggctatc 1021 caaatgttgg caagagctct gtgataaata cattgcgttc taagaaagtt tgcaacgtgg 1081 ctcccattgc aggtgaaaca aaggtctggc agtatattac tttgatgcgt cggatattcc 1141 tgattgactg tccaggtgtg gtttacccct ctgaggactc cgagacagac attgtgctaa 1201 aaggagttgt tcaagtagaa aaaattaaga gtcctgaaga ccacattggt gctgtacttg 1261 aacgagcaaa gccagaatat atcagcaaaa catacaagat tgattcttgg gagaatgctg 1321 aggactttct tgagaagctc gctttccgga ctgggaagtt actaaagggt ggagagcccg 1381 acttgcagac tgtgggtaag atggtcctca atgactggca gaggggccgg attcctttct 1441 ttgtcaagcc acccaatgca gagccacttg tggcccccca gcttctaccc tcctcatctt 1501 tggaagttgt cccagaagca gcccagaaca atccagggga ggaagtcaca gaaactgcag 1561 gtgaagggtc agaatccatc attaaggaag aaacagaaga gaacagtcac tgtgatgcta 1621 acacagagat gcagcagatt ctcacacgag ttcggcagaa ctttggtaaa atcaacgtgg 1681 tgcctcagtt ttctggggat gacctggttc ctgtggaggt gtcagatctt gaggaagagc 1741 ttgagagctt ttctgatgaa gaggaggagg aacaggagca acaaagagat gatgcggaag 1801 agtcttcctc ggagcctgag gaggaaaatg tgggaaacga caccaaagcc gttattaaag 1861 cactggatga gaagattgcc aaatatcaga agtttctaga caaagccaaa gccaaaaagt 1921 tttcagcagt cagaatatcc aagggactga gtgaaaagat atttgcaaaa cctgaagaac 1981 aaagaaaaac actggaagaa gatgtagatg acagagcacc ttccaaaaag ggaaagaagc 2041 ggaaggcaca aagggaagag gaacaggaac attcaaataa agctcccagg gcgcttacat 2101 caaaagaacg gaggcgagca gtacgacagc aacggccgaa aaaagttggt gtgcgctact 2161 atgaaacaca caacgtgaaa aataggaaca ggaacaaaaa gaagaccaat gactcagagg 2221 gacagaaaca caaacgcaaa aaattcagac aaaagcagta cccaactttc ttgtacaaag 2281 tggttggtaa gcctatccct aaccctctcc tcggtctcga ttctacgtag taatgagcta 2341 gcgctaaccg gtggcgcgtt aagtcgacaa tcaacctctg gatta