Construct: ORF ccsbBroad304_08158
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002473.1_s304c1, BRDN0000411498
- Derived from:
- ccsbBroadEn_08158
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TNPO2 (30000)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_08158
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 30000 | TNPO2 | transportin 2 | NM_001136195.2 | 99.9% | 99.8% | 2618T>N |
| 2 | human | 30000 | TNPO2 | transportin 2 | NM_013433.5 | 99.9% | 99.8% | 2618T>N |
| 3 | human | 30000 | TNPO2 | transportin 2 | NM_001136196.2 | 98.8% | 98.7% | 2303_2332del;2648T>N |
| 4 | mouse | 212999 | Tnpo2 | transportin 2 (importin 3, ... | XM_011248342.2 | 91.6% | 99.5% | (many diffs) |
| 5 | mouse | 212999 | Tnpo2 | transportin 2 (importin 3, ... | XM_011248345.2 | 91.6% | 99.5% | (many diffs) |
| 6 | mouse | 212999 | Tnpo2 | transportin 2 (importin 3, ... | XM_017312647.1 | 91.6% | 99.5% | (many diffs) |
| 7 | mouse | 212999 | Tnpo2 | transportin 2 (importin 3, ... | XM_017312648.1 | 91.6% | 99.5% | (many diffs) |
| 8 | mouse | 212999 | Tnpo2 | transportin 2 (importin 3, ... | NM_001122843.1 | 90.5% | 98.4% | (many diffs) |
| 9 | mouse | 212999 | Tnpo2 | transportin 2 (importin 3, ... | NM_145390.4 | 90.5% | 98.4% | (many diffs) |
| 10 | mouse | 212999 | Tnpo2 | transportin 2 (importin 3, ... | XM_011248343.2 | 90.5% | 98.4% | (many diffs) |
| 11 | mouse | 212999 | Tnpo2 | transportin 2 (importin 3, ... | XM_011248344.2 | 90.5% | 98.4% | (many diffs) |
| 12 | mouse | 212999 | Tnpo2 | transportin 2 (importin 3, ... | XM_017312649.1 | 78.7% | 85.7% | (many diffs) |
| 13 | mouse | 212999 | Tnpo2 | transportin 2 (importin 3, ... | XM_017312650.1 | 78.7% | 85.7% | (many diffs) |
| 14 | mouse | 212999 | Tnpo2 | transportin 2 (importin 3, ... | XM_011248346.2 | 77.8% | 84.8% | (many diffs) |
| 15 | mouse | 212999 | Tnpo2 | transportin 2 (importin 3, ... | XR_878792.2 | 38.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2727
- ORF length:
- 2661
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgga ctggcagcca gacgagcagg gcctgcagca ggtcctgcag ctgctcaaag 121 actcacagtc gcccaacaca gccactcagc gcatcgtgca ggataaactc aaacaactca 181 atcagtttcc tgacttcaac aactacctga ttttcgtcct gaccagactc aagtcagaag 241 atgagccaac gcgctctctc agtggcctca tcctcaagaa caacgtgaag gcacactatc 301 agagcttccc accccctgtg gcagacttca tcaaacagga gtgtctcaac aacattggcg 361 atgcctcctc gctcatccga gccaccattg gcattctcat caccaccatc gcttccaagg 421 gtgagctgca gatgtggccc gagctgctgc cccagctctg caacctgctt aactcggagg 481 attacaacac ttgtgaggga gcctttggag ccctgcagaa gatctgtgaa gactcatcag 541 agcttctgga cagtgacgcc ctcaacaggc ccctcaacat catgatcccc aagttcctgc 601 agttcttcaa gcactgcagt cccaagatcc ggtcccacgc catcgcctgc gtgaaccagt 661 tcatcatgga ccgggcccag gcgctgatgg acaatattga caccttcatc gagcacctat 721 ttgccctggc tgtggatgat gaccccgagg tgcggaagaa tgtgtgccgt gccctggtga 781 tgcttctgga agtgcggatt gacaggctca tcccccacat gcacagcatc atccagtaca 841 tgctgcagag gacccaggac catgatgaga acgttgccct tgaggcctgt gagttctggc 901 tgacgctggc cgagcagccc atctgcaagg aagtcctggc ctcccatctg gtccagttga 961 tccccatctt ggtgaatggg atgaagtact cggaaattga catcatcctg ctcaaggggg 1021 atgtggagga ggatgaggct gtccccgaca gtgagcagga catcaagcca cgcttccaca 1081 agtcacgcac ggtcacactg ccccacgagg ctgagcggcc tgatggctcc gaggacgcgg 1141 aggatgacga tgatgatgat gctctgtccg actggaattt gaggaagtgc tcagcggctg 1201 cactggacgt cctcgccaat gtcttccggg aggaactgct gccccaccta ctcccactac 1261 tcaaaggcct cctcttccac cccgagtggg tggtcaagga gtcgggcatc ctggtgctgg 1321 gcgccattgc tgagggctgc atgcagggca tggtgcccta cctgcctgag ctgatcccgc 1381 acctgatcca gtgcctgtcg gataagaagg ccttggtccg ctccatcgcc tgctggacgc 1441 tgagccgcta tgcccactgg gtggtcagcc agccacccga catgcacctc aagcccctga 1501 tgacagagct gctcaaacgc atcctggatg gcaacaagag ggtacaggag gcggcctgca 1561 gtgcttttgc caccctggag gaagaggcct gcacggagct ggtgccctac ctcagctaca 1621 tcctggacac ccttgtcttt gcctttggga aataccagca caagaacctg ctcatcctct 1681 atgacgccat tggcaccctg gccgactctg taggccacca cctcaaccag ccggaataca 1741 tccagaagct gatgccccca ctgatccaga agtggaatga gctcaaggac gaagacaagg 1801 acctcttccc cctgctggag tgtctgtcat cggtggccac cgccctgcag agtggcttcc 1861 tgccttactg tgagcccgtc taccagcgct gtgtcaccct ggtgcagaag acactggctc 1921 aggccatgat gtacacccag caccctgagc agtatgaggc tcccgacaag gacttcatga 1981 tcgtagcact ggatctgctc agcggcctgg ccgagggcct gggtggtcac gtggagcagc 2041 tggtggcccg cagcaacatc atgacattgc tgttccagtg catgcaggac tcgatgcctg 2101 aggtccggca gagctccttt gccctcctgg gagacctcac caaagcctgc ttcatccatg 2161 tcaagccctg tatcgccgag ttcatgccca ttctgggcac caacctgaac ccagagttca 2221 tctccgtctg caacaacgcc acctgggcca ttggtgaaat ctgcatgcag atgggggcag 2281 agatgcagcc ttatgtgcag atggtcctca acaacctggt ggaaatcatt aaccgaccca 2341 acacacccaa gacactgctg gaaaacacag ccatcaccat cggccgcttg ggctacgtgt 2401 gcccccagga ggtggcaccc atgctgcagc agttcatccg gccttggtgc acgtccctca 2461 ggaacatcag ggacaacgag gagaaggact cagccttccg cggcatctgc atgatgatcg 2521 gtgtcaaccc ggggggcgtt gtgcaggact ttattttctt ctgcgatgct gtagcctcct 2581 gggtgagccc gaaggatgac cttcgggaca tgttttataa gattctccac ggcttcaaag 2641 accaagttgg ggaagataac tggcagcagt tctctgagca atncccgccg ctgctcaagg 2701 agaggctggc ggctttctat ggggtctacc caactttctt gtacaaagtg gttggtaagc 2761 ctatccctaa ccctctcctc ggtctcgatt ctacgtagta atgagctagc gctaaccggt 2821 ggcgcgttaa gtcgacaatc aacctctgga tta