Construct: ORF ccsbBroad304_08261

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF004012.1_s304c1, BRDN0000399458
Derived from:
ccsbBroadEn_08261
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
NT5C3A (51251)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_08261
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 51251 NT5C3A 5'-nucleotidase, cytosolic ... NM_001166118.3 99.5% 99.6% 0_1insATG;240T>C
2 human 51251 NT5C3A 5'-nucleotidase, cytosolic ... NM_001356996.2 99.5% 99.6% 0_1insATG;240T>C
3 human 51251 NT5C3A 5'-nucleotidase, cytosolic ... NM_001374336.1 99.5% 99.6% 0_1insATG;240T>C
4 human 51251 NT5C3A 5'-nucleotidase, cytosolic ... NM_001374337.1 99.5% 99.6% 0_1insATG;240T>C
5 human 51251 NT5C3A 5'-nucleotidase, cytosolic ... NM_001002009.3 96.1% 96.2% 1_33del;276T>C
6 human 51251 NT5C3A 5'-nucleotidase, cytosolic ... NM_016489.13 96.1% 96.2% 1_33del;276T>C
7 human 51251 NT5C3A 5'-nucleotidase, cytosolic ... NM_001374339.1 89.6% 89.5% (many diffs)
8 human 51251 NT5C3A 5'-nucleotidase, cytosolic ... NM_001002010.4 86.3% 86.4% 1_135del;378T>C
9 human 51251 NT5C3A 5'-nucleotidase, cytosolic ... NM_001374335.1 85.2% 84.3% (many diffs)
10 human 51251 NT5C3A 5'-nucleotidase, cytosolic ... NM_001374338.1 66% 66.1% 1_135del;378T>C;693_694ins201
11 mouse 107569 Nt5c3 5'-nucleotidase, cytosolic III XM_006505275.2 86.1% 93% (many diffs)
12 mouse 107569 Nt5c3 5'-nucleotidase, cytosolic III XM_006505276.4 85.7% 92.6% (many diffs)
13 mouse 107569 Nt5c3 5'-nucleotidase, cytosolic III XM_006505274.2 84.1% 90.7% (many diffs)
14 mouse 107569 Nt5c3 5'-nucleotidase, cytosolic III NM_001252374.1 82.9% 89.5% (many diffs)
15 mouse 107569 Nt5c3 5'-nucleotidase, cytosolic III NM_001360963.1 82.9% 89.5% (many diffs)
16 mouse 107569 Nt5c3 5'-nucleotidase, cytosolic III XM_006505273.2 82.9% 89.5% (many diffs)
17 mouse 107569 Nt5c3 5'-nucleotidase, cytosolic III XM_011241111.2 82.9% 89.5% (many diffs)
18 mouse 107569 Nt5c3 5'-nucleotidase, cytosolic III XM_030255051.1 82.9% 89.5% (many diffs)
19 mouse 107569 Nt5c3 5'-nucleotidase, cytosolic III NM_026004.3 74.4% 80.3% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
924
ORF length:
858
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgat gccagaattc cagaaaagtt cagttcgaat caagaaccct acaagagtag
121aagaaattat ctgtggtctt atcaaaggag gagctgccaa acttcagata ataacggact
181ttgatatgac actcagtaga ttttcatata aagggaaaag atgcccaaca tgtcataata
241tcattgacaa ctgtaagctg gttacagatg aatgtagaaa aaagttattg caactaaagg
301aaaaatacta cgctattgaa gttgatcctg ttcttactgt agaagagaag tacccttata
361tggtggaatg gtatactaaa tcacatggtt tgcttgttca gcaagcttta ccaaaagcta
421aacttaaaga aattgtggca gaatctgacg ttatgctcaa agaaggatat gagaatttct
481ttgataagct ccaacaacat agcatccccg tgttcatatt ttcggctgga atcggcgatg
541tactagagga agttattcgt caagctggtg tttatcatcc caatgtcaaa gttgtgtcca
601attttatgga ttttgatgaa actggggtgc tcaaaggatt taaaggagaa ctaattcatg
661tatttaacaa acatgatggt gccttgagga atacagaata tttcaatcaa ctaaaagaca
721atagtaacat aattcttctg ggagactccc aaggagactt aagaatggca gatggagtgg
781ccaatgttga gcacattctg aaaattggat atctaaatga tagagtggat gagcttttag
841aaaagtacat ggactcttat gatattgttt tagtacaaga tgaatcatta gaagtagcca
901actctatttt acagaagatt ctatacccaa ctttcttgta caaagtggtt ggtaagccta
961tccctaaccc tctcctcggt ctcgattcta cgtagtaatg agctagcgct aaccggtggc
1021gcgttaagtc gacaatcaac ctctggatta

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