Construct: ORF ccsbBroad304_08334
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003747.1_s304c1, BRDN0000400874
- Derived from:
- ccsbBroadEn_08334
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CDK5RAP1 (51654)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_08334
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 51654 | CDK5RAP1 | CDK5 regulatory subunit ass... | NM_016408.4 | 99.9% | 100% | 432A>G |
| 2 | human | 51654 | CDK5RAP1 | CDK5 regulatory subunit ass... | NM_001278167.1 | 99.7% | 99.8% | 432A>G;1390_1391insAGA |
| 3 | human | 51654 | CDK5RAP1 | CDK5 regulatory subunit ass... | NM_001365728.1 | 97.6% | 97.6% | 432A>G;874_915del |
| 4 | human | 51654 | CDK5RAP1 | CDK5 regulatory subunit ass... | NM_016082.4 | 94.1% | 92.8% | (many diffs) |
| 5 | human | 51654 | CDK5RAP1 | CDK5 regulatory subunit ass... | NM_001278169.1 | 91.8% | 87% | (many diffs) |
| 6 | human | 51654 | CDK5RAP1 | CDK5 regulatory subunit ass... | NM_001278168.1 | 86.8% | 86.8% | 432A>G;874_875ins231 |
| 7 | human | 51654 | CDK5RAP1 | CDK5 regulatory subunit ass... | XR_001754289.2 | 81.5% | (many diffs) | |
| 8 | human | 51654 | CDK5RAP1 | CDK5 regulatory subunit ass... | XM_011528857.2 | 81.2% | 81.2% | 0_1ins330;102A>G |
| 9 | human | 51654 | CDK5RAP1 | CDK5 regulatory subunit ass... | XM_011528856.3 | 80% | 75.5% | (many diffs) |
| 10 | human | 51654 | CDK5RAP1 | CDK5 regulatory subunit ass... | XM_011528858.1 | 78.3% | 77% | 0_1ins277;25_26ins104;51A>G |
| 11 | human | 51654 | CDK5RAP1 | CDK5 regulatory subunit ass... | XM_011528859.2 | 78.3% | 77% | 0_1ins277;25_26ins104;51A>G |
| 12 | human | 51654 | CDK5RAP1 | CDK5 regulatory subunit ass... | XR_001754290.2 | 77% | (many diffs) | |
| 13 | human | 51654 | CDK5RAP1 | CDK5 regulatory subunit ass... | XM_024451892.1 | 68.8% | 68.8% | 0_1ins549 |
| 14 | human | 51654 | CDK5RAP1 | CDK5 regulatory subunit ass... | XM_024451893.1 | 68.8% | 68.8% | 0_1ins549 |
| 15 | human | 51654 | CDK5RAP1 | CDK5 regulatory subunit ass... | XM_024451894.1 | 68.8% | 68.8% | 0_1ins549 |
| 16 | human | 51654 | CDK5RAP1 | CDK5 regulatory subunit ass... | XM_024451895.1 | 68.8% | 68.8% | 0_1ins549 |
| 17 | human | 51654 | CDK5RAP1 | CDK5 regulatory subunit ass... | XM_011528860.1 | 56.7% | 56.7% | 0_1ins762 |
| 18 | human | 51654 | CDK5RAP1 | CDK5 regulatory subunit ass... | XM_017027876.2 | 56.7% | 56.7% | 0_1ins762 |
| 19 | human | 51654 | CDK5RAP1 | CDK5 regulatory subunit ass... | XM_024451896.1 | 56.7% | 56.7% | 0_1ins762 |
| 20 | human | 51654 | CDK5RAP1 | CDK5 regulatory subunit ass... | XM_024451897.1 | 56.7% | 56.7% | 0_1ins762 |
| 21 | human | 51654 | CDK5RAP1 | CDK5 regulatory subunit ass... | XM_017027877.1 | 53.5% | 51.2% | (many diffs) |
| 22 | mouse | 66971 | Cdk5rap1 | CDK5 regulatory subunit ass... | NM_025876.2 | 85.5% | 87.2% | (many diffs) |
| 23 | mouse | 66971 | Cdk5rap1 | CDK5 regulatory subunit ass... | XR_001783168.1 | 71.5% | (many diffs) | |
| 24 | mouse | 66971 | Cdk5rap1 | CDK5 regulatory subunit ass... | XR_001783167.1 | 55.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1827
- ORF length:
- 1761
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgca ccctttacag tgtgtcctcc aagtgcagag gtctctgggg tggggaccat 121 tggcctctgt gtcttggctg tcgctgagga tgtgcagggc acacagcagt ctctctagta 181 ccatgtgtcc cagtccagag aggcaggagg atggagctcg gaaggatttc agctccaggc 241 tggctgctgg accgactttt caacattttt taaaaagtgc ctcagctcct caggagaagc 301 tgtcttcaga agtggaagac ccacctccct atctcatgat ggatgaactt cttggaaggc 361 agagaaaagt ctacctcgag acctatggct gccagatgaa tgtgaatgac acagagatag 421 cctggtccat cttacagaag agtggctacc tgcggaccag taacctccaa gaggcagatg 481 tgattctcct tgtcacgtgc tctatcaggg agaaggctga gcagaccatc tggaaccgtt 541 tacatcagct taaagccttg aagacaaggc ggccccgctc ccgggttcct ctgaggattg 601 gaattctagg ctgcatggct gagaggttga aggaggagat tctcaacaga gagaaaatgg 661 tagatatttt ggctggtcct gatgcctacc gggaccttcc ccggctgctg gctgttgctg 721 agtcgggcca gcaagctgcc aacgtgctgc tctctctgga cgagacctat gctgatgtca 781 tgccagtcca gacaagcgcc agtgccacgt ctgcctttgt gtcaatcatg cgaggctgtg 841 acaacatgtg tagctactgc attgttcctt tcacccgggg cagggagagg agtcggccta 901 ttgcctccat tctagaggaa gtgaagaagc tttctgagca ggggctgaaa gaagtgacac 961 ttcttggtca gaatgttaat agttttcggg acaattcgga ggtccagttc aacagtgcag 1021 tgcctaccaa tctcagtcgt ggctttacca ccaactataa aaccaagcaa ggaggacttc 1081 gttttgctca tcttctggat caggtctcca gagtagatcc tgaaatgagg atccgtttta 1141 cctctcccca ccccaaggat tttcctgatg aggttctgca gctgattcat gagagagata 1201 acatctgtaa acagatccac ctgccagccc agagtggaag cagccgtgtg ttggaggcca 1261 tgcggagggg atattcaaga gaagcttatg tggagttagt tcaccatatt agagaatcta 1321 ttccaggtgt gagcctcagc agcgatttca ttgctggctt ttgtggtgag acggaggaag 1381 atcacgtcca gacagtctct ttgctccggg aagttcagta caacatgggc ttcctctttg 1441 cctacagcat gagacagaag acacgggcat atcataggct gaaggatgat gtcccggaag 1501 aggtaaaatt aaggcgtttg gaggaactca tcactatctt ccgagaagaa gcaacaaaag 1561 ccaatcagac ctctgtgggc tgtacccagt tggtgctagt ggaagggctc agtaaacgct 1621 ctgccactga cctgtgtggc aggaatgatg gaaaccttaa ggtgatcttc cctgatgcag 1681 agatggagga tgtcaataac cctgggctca gggtcagagc ccagcctggg gactatgtgc 1741 tggtgaagat cacctcagcc agttctcaga cacttagggg acatgttctc tgcaggacca 1801 ctctgaggga ctcttctgca tattgctgcc caactttctt gtacaaagtg gttggtaagc 1861 ctatccctaa ccctctcctc ggtctcgatt ctacgtagta atgagctagc gctaaccggt 1921 ggcgcgttaa gtcgacaatc aacctctgga tta