Construct: ORF ccsbBroad304_08358

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF008628.1_s304c1, BRDN0000462106
Derived from:
ccsbBroadEn_08358
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
CPSF2 (53981)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_08358
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 53981 CPSF2 cleavage and polyadenylatio... NM_001322272.2 99.8% 99.7% (many diffs)
2 human 53981 CPSF2 cleavage and polyadenylatio... NM_017437.3 99.8% 99.7% (many diffs)
3 human 53981 CPSF2 cleavage and polyadenylatio... NM_001322270.2 93.3% 93.2% (many diffs)
4 human 53981 CPSF2 cleavage and polyadenylatio... NM_001322271.2 80.2% 80.1% (many diffs)
5 mouse 51786 Cpsf2 cleavage and polyadenylatio... NM_016856.3 88.5% 97.6% (many diffs)
6 mouse 51786 Cpsf2 cleavage and polyadenylatio... XM_006516071.3 88.5% 97.6% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
2415
ORF length:
2346
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcaccat gacgtctatt atcaaattaa ctaccctttc tggggtccaa gaagaatctg
121ccctttgcta tcttctccaa gttgatgagt ttagattttt attggactgt ggctgggatg
181agcacttttc tatggatatt attgattccc tgaggaagca tgttcaccag attgatgcag
241tgctgttgtc tcaccctgat cctctccacc ttggtgccct cccgtatgct gtcggaaagt
301tgggtctgaa ctgtgctatc tatgcaacca ttcctgttta taaaatggga cagatgttca
361tgtatgatct ttatcagtct cgacacaata cagaagattt tacactcttt acattagatg
421atgtggatgc agcctttgat aaaatacagc agctaaaatt ctctcagatt gtgaatttga
481aaggtaaagg acatggcctg tctatcacac ctctgccagc tggtcatatg ataggtggaa
541caatatggaa aatagtcaaa gatggagaag aagaaattgt ttatgcagtt gacttcaacc
601acaagaggga gatccattta aatggatgtt ccctggaaat gctaagcagg ccttccctac
661ttatcacaga ttcattcaat gctacatatg tacagcctag aagaaaacag agagatgagc
721agcttctgac aaatgtcctg gaaacacttc gaggtgatgg aaatgtgtta atagcagtgg
781acacagcagg cagagttttg gaacttgctc aacttcttga tcagatttgg aggactaaag
841atgcaggatt gggtgtttac tcattggcac tcctaaataa tgtcagttac aatgtggtgg
901agttttctaa gtcccaggta gaatggatga gtggtaaatt gatgagatgt tttgaagaca
961aaagaaataa tccgtttcag tttcgccatc tctctttatg tcatggtctt tctgacttgg
1021cccgtgtacc tagccctaaa gttgtacttg ccagccaacc tgacctggaa tgcggatttt
1081caagggatct ctttattcag tggtgtcagg accctaaaaa ctcaatcatt ctaacctaca
1141gaactactcc tgggacttta gcacgtttcc taattgataa tccttctgaa aaaattacag
1201aaatagagtt gaggaaacgt gtgaagcttg aagggaaaga acttgaagaa tacttggaaa
1261aagagaaact aaagaaagaa gctgccaaaa agcttgagca gtcaaaagag gcagatatag
1321attccagtga tgagagtgat attgaggaag atattgacca gccatcagct cataagacga
1381agcatgactt gatgatgaaa ggtgaaggca gtcgtaaagg aagttttttc aaacaggcaa
1441aaaagtccta tcctatgttt cctgccccag aagaaagaat taaatgggat gaatatggag
1501agattatcaa accagaggat ttcttagtgc cagagcttca agctactgaa gaagaaaaaa
1561gcaaattaga atctggtttg acaaatggag atgaacctat ggatcaggat ttatctgatg
1621ttcctactaa atgtatttct acaacagagt ctattgaaat aaaagcccgg gttacctaca
1681tagactatga aggacgctct gatggggatt ccattaaaaa aatcattaat cagatgaaac
1741cacgacagtt gatcatcgtc catggcccac cagaggccag tcaagatctg gcagagtgct
1801gtcgcgcctt tggtgggaaa gatattaaag tgtacatgcc aaagctacat gaaacagttg
1861atgccactag tgaaactcac atctaccagg tgaggttaaa agactcactt gtcagctctc
1921ttcagttttg taaggcaaaa gatgctgaat tagcttggat agatggtgtc ttagatatga
1981gagtttccaa agtggacaca ggggttattt tagaagaagg agaactaagg gatgatggag
2041aagactcaga gatgcaagtg gaagctccct cagattctag cgttatagca caacaaaagg
2101ccatgaaaag tttgttcgga gatgatgaaa aagaaacagg tgaagaaagt gagatcattc
2161ctactttgga acccttgcca cctcatgagg ttcctggaca tcagtcagtt tttatgaatg
2221aaccaaggct gtcagacttc aagcaagttc tcttacggga gggaattcaa gctgaatttg
2281taggaggtgt acttgtttgc aacaatcaag tagcagtccg cagaacggaa actggacgca
2341ttggattaga aggctgcctt tgtcaagatt tttataggat aagagacctt ttatatgaac
2401aatatgccat tgtattgcca actttcttgt acaaagtggt tggtaagcct atccctaacc
2461ctctcctcgg tctcgattct acgtagtaat gagctagcgc taaccggtgg cgcgttaagt
2521cgacaatcaa cctctggatt a

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