Transcript: Human NM_001322270.2

Homo sapiens cleavage and polyadenylation specific factor 2 (CPSF2), transcript variant 2, mRNA.

Source:
NCBI, updated 2019-05-31
Taxon:
Homo sapiens (human)
Gene:
CPSF2 (53981)
Length:
12850
CDS:
254..2449

Additional Resources:

NCBI RefSeq record:
NM_001322270.2
NBCI Gene record:
CPSF2 (53981)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001322270.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000075007 CCCTCAGATTCTAGCGTTATA pLKO.1 2099 CDS 100% 13.200 18.480 N CPSF2 n/a
2 TRCN0000075006 CCGGGTTACCTACATAGACTA pLKO.1 1699 CDS 100% 4.950 6.930 N CPSF2 n/a
3 TRCN0000075005 CGGGTTACCTACATAGACTAT pLKO.1 1700 CDS 100% 4.950 6.930 N CPSF2 n/a
4 TRCN0000413700 ATATGATAGGTGGAACAATAT pLKO_005 711 CDS 100% 13.200 9.240 N CPSF2 n/a
5 TRCN0000075003 GCCCTCTTCTTTACTCTTAAT pLKO.1 3608 3UTR 100% 13.200 9.240 N CPSF2 n/a
6 TRCN0000417817 GCTATGACTCTCTCCAGTTTG pLKO_005 2928 3UTR 100% 10.800 7.560 N CPSF2 n/a
7 TRCN0000419365 GTCTGAACTGTGCTATCTATG pLKO_005 489 CDS 100% 10.800 7.560 N CPSF2 n/a
8 TRCN0000428508 GTGTACTTGTTTGCAACAATC pLKO_005 2319 CDS 100% 10.800 7.560 N CPSF2 n/a
9 TRCN0000075004 CCTACTTATCACAGATTCATT pLKO.1 841 CDS 100% 5.625 3.938 N CPSF2 n/a
10 TRCN0000119561 CTCGACACAATACAGAAGATT pLKO.1 564 CDS 100% 5.625 3.938 N Cpsf2 n/a
11 TRCN0000317837 CTCGACACAATACAGAAGATT pLKO_005 564 CDS 100% 5.625 3.938 N Cpsf2 n/a
12 TRCN0000423591 GGAATGATAAGTGAGAATTAT pLKO_005 2831 3UTR 100% 15.000 9.000 N CPSF2 n/a
13 TRCN0000434454 AGACCAACCTGGGCAACATAG pLKO_005 10642 3UTR 100% 10.800 5.400 Y SULT1A1 n/a
14 TRCN0000007228 CACCTGTAATCCCAGCACTTT pLKO.1 8689 3UTR 100% 4.950 2.475 Y CFLAR n/a
15 TRCN0000166635 CACCTGTAATCCCAGCACTTT pLKO.1 8689 3UTR 100% 4.950 2.475 Y C19orf31 n/a
16 TRCN0000165534 GAGACAGGGTTTCACCATGTT pLKO.1 3793 3UTR 100% 4.950 2.475 Y n/a
17 TRCN0000140536 GCCAACATGGTGAAACCTCAT pLKO.1 8762 3UTR 100% 4.050 2.025 Y TLCD4 n/a
18 TRCN0000155836 CCCAAAGTGCTGGGATTACAA pLKO.1 3866 3UTR 100% 5.625 2.813 Y KLHL30 n/a
19 TRCN0000021429 CACACCTGTAATCCCAGCATT pLKO.1 8687 3UTR 100% 4.950 2.475 Y ERN2 n/a
20 TRCN0000138998 CACACCTGTAATCCCAGCATT pLKO.1 8687 3UTR 100% 4.950 2.475 Y P3H4 n/a
21 TRCN0000344020 CACACCTGTAATCCCAGCATT pLKO_005 8687 3UTR 100% 4.950 2.475 Y P3H4 n/a
22 TRCN0000141025 CCCAAAGTGCTGGGATTACTT pLKO.1 3866 3UTR 100% 5.625 2.813 Y EID2B n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001322270.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_08358 pDONR223 100% 93.3% 93.2% None (many diffs) n/a
2 ccsbBroad304_08358 pLX_304 0% 93.3% 93.2% V5 (many diffs) n/a
3 TRCN0000476172 CACAGGGTCAACGAGCAAGTATCA pLX_317 16% 93.3% 93.2% V5 (many diffs) n/a
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