Construct: ORF ccsbBroad304_08359
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003331.1_s304c1, BRDN0000408839
- Derived from:
- ccsbBroadEn_08359
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- BRWD1 (54014)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_08359
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 54014 | BRWD1 | bromodomain and WD repeat d... | NM_001007246.2 | 99.7% | 99.1% | 247C>G |
| 2 | human | 54014 | BRWD1 | bromodomain and WD repeat d... | XM_017028375.1 | 11.2% | 10.9% | (many diffs) |
| 3 | human | 54014 | BRWD1 | bromodomain and WD repeat d... | XR_001754864.1 | 9.4% | (many diffs) | |
| 4 | human | 54014 | BRWD1 | bromodomain and WD repeat d... | NM_033656.4 | 5.1% | 5% | (many diffs) |
| 5 | human | 54014 | BRWD1 | bromodomain and WD repeat d... | XM_011529611.1 | 5.1% | 5% | (many diffs) |
| 6 | human | 54014 | BRWD1 | bromodomain and WD repeat d... | NM_018963.5 | 5% | 4.9% | (many diffs) |
| 7 | mouse | 93871 | Brwd1 | bromodomain and WD repeat d... | NM_176928.1 | 90.2% | 97.5% | (many diffs) |
| 8 | mouse | 93871 | Brwd1 | bromodomain and WD repeat d... | NM_001103179.1 | 4.6% | 5% | (many diffs) |
| 9 | mouse | 93871 | Brwd1 | bromodomain and WD repeat d... | NM_145125.3 | 4.5% | 4.9% | (many diffs) |
| 10 | mouse | 93871 | Brwd1 | bromodomain and WD repeat d... | XM_011246157.2 | 4.5% | 4.9% | (many diffs) |
| 11 | mouse | 93871 | Brwd1 | bromodomain and WD repeat d... | XM_017317169.1 | 3.9% | 4.2% | (many diffs) |
| 12 | mouse | 93871 | Brwd1 | bromodomain and WD repeat d... | XM_017317170.1 | 3.9% | 4.2% | (many diffs) |
| 13 | mouse | 93871 | Brwd1 | bromodomain and WD repeat d... | XM_006523114.3 | 3.8% | 4.1% | (many diffs) |
| 14 | mouse | 93871 | Brwd1 | bromodomain and WD repeat d... | XM_006523113.1 | 3.8% | 4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 429
- ORF length:
- 360
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcggagccg tcgtccgccc gacgcccggt gcctctcatc gagtcggagc 121 tgtacttcct tatcgcccgg tacctatcgg cgggcccgtg tcggagagcg gcccaggtgc 181 tggtgcagga gctggagcag taccagttgt tgccgaagag attggactgg gagggcaacg 241 agcacaacag gagctacgag gagttggtct tgtccaataa gcatgtggct cctgatcatc 301 ttttgcaaat ctgcgagcgc atcggtccta tgttggataa agaaattcca cccagtattt 361 caagagtcac ttctttactt ggtgcaggaa ggcagtcttt gctacgtaca gcaaaaggta 421 ccttaatttt gccaactttc ttgtacaaag tggttggtaa gcctatccct aaccctctcc 481 tcggtctcga ttctacgtag taatgagcta gcgctaaccg gtggcgcgtt aagtcgacaa 541 tcaacctctg gatta