Construct: ORF ccsbBroad304_08407

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF013699.1_s304c1, BRDN0000403440
Derived from:
ccsbBroadEn_08407
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
FBLIM1 (54751)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_08407
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 54751 FBLIM1 filamin binding LIM protein 1 NM_001350151.2 99.9% 99.7% 572C>T
2 human 54751 FBLIM1 filamin binding LIM protein 1 NM_017556.4 99.9% 99.7% 572C>T
3 human 54751 FBLIM1 filamin binding LIM protein 1 XM_011541617.2 99.9% 99.7% 572C>T
4 human 54751 FBLIM1 filamin binding LIM protein 1 XM_017001525.1 99.9% 99.7% 572C>T
5 human 54751 FBLIM1 filamin binding LIM protein 1 XM_011541618.3 84% 78.8% 572C>T;888_889ins118;942_943ins59
6 human 54751 FBLIM1 filamin binding LIM protein 1 NM_001024215.1 81.7% 76.6% (many diffs)
7 human 54751 FBLIM1 filamin binding LIM protein 1 XM_005245900.1 81.7% 76.6% (many diffs)
8 human 54751 FBLIM1 filamin binding LIM protein 1 XM_005245901.1 81.7% 76.6% (many diffs)
9 human 54751 FBLIM1 filamin binding LIM protein 1 XM_005245902.1 81.7% 76.6% (many diffs)
10 human 54751 FBLIM1 filamin binding LIM protein 1 XM_005245903.1 81.7% 76.6% (many diffs)
11 human 54751 FBLIM1 filamin binding LIM protein 1 XM_006710704.3 81.7% 76.6% (many diffs)
12 human 54751 FBLIM1 filamin binding LIM protein 1 XM_006710705.1 81.7% 76.6% (many diffs)
13 human 54751 FBLIM1 filamin binding LIM protein 1 XM_011541616.2 81.7% 76.6% (many diffs)
14 human 54751 FBLIM1 filamin binding LIM protein 1 XM_017001519.2 81.7% 76.6% (many diffs)
15 human 54751 FBLIM1 filamin binding LIM protein 1 XM_017001520.2 81.7% 76.6% (many diffs)
16 human 54751 FBLIM1 filamin binding LIM protein 1 XM_017001521.2 81.7% 76.6% (many diffs)
17 human 54751 FBLIM1 filamin binding LIM protein 1 XM_017001523.1 81.7% 76.6% (many diffs)
18 human 54751 FBLIM1 filamin binding LIM protein 1 XM_017001524.1 81.7% 76.6% (many diffs)
19 human 54751 FBLIM1 filamin binding LIM protein 1 XM_017001526.2 81.1% 79.4% (many diffs)
20 human 54751 FBLIM1 filamin binding LIM protein 1 XM_024447751.1 80.2% 78.6% (many diffs)
21 human 54751 FBLIM1 filamin binding LIM protein 1 NM_001024216.3 73.9% 73.7% 248_249ins291;281C>T
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1185
ORF length:
1119
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatggc ctcaaagcct gagaagaggg tggcatcgtc tgtctttatc accctggcac
121ccccgcgccg cgatgtggcc gtggcggagg aagtgaggca ggcagtttgt gaggcccggc
181gtggccgccc ctgggaggct cctgccccca tgaagacacc cgaggctggc ttggcgggga
241ggcccagccc ctggacaacc cctggcagag ctgcagccac agtgccggct gcacctatgc
301agctcttcaa tggaggatgc ccaccccctc ctcctgtcct ggatggtgag gacgtgcttc
361ctgacctgga cctcctccca ccccctccac cgccccctcc agtgcttctg ccttctgaag
421aggaggctcc tgctccaatg ggggcctcac tcattgcaga cttagagcag ctgcacctgt
481ccccgccccc gcccccacca caggccccag cggagggacc ttcagtccag cccggtcccc
541tcaggcccat ggaggaagag ctgccacctc ccccggcaga acctgttgag aaaggggcat
601ccacagacat ctgtgccttc tgccacaaga ccgtgttccc ccgagagctg gctgtggagg
661ccatgaagag gcagtaccat gcccagtgct tcacgtgccg cacctgccgc cgccagctgg
721ctgggcagag cttctaccag aaggatgggc gacccctctg cgaaccctgc taccaggaca
781cactggagag gtgcggcaag tgtggcgagg tggtccggga ccacatcatc agggccctgg
841gccaggcctt ccacccctcc tgcttcacgt gtgtgacctg cgcccggtgc attggggatg
901agagctttgc cctgggcagc cagaacgagg tgtactgcct ggacgacttc tacaggaaat
961tcgcccccgt ctgcagcatc tgtgaaaatc ccatcatccc tcgggatggg aaagatgcct
1021tcaaaatcga atgcatggga agaaacttcc atgaaaattg ctacaggtgt gaggactgca
1081ggatcctcct gtctgtcgag cccacggacc aaggctgcta ccccctgaac aaccatctct
1141tctgcaagcc atgccatgtg aagcggagtg ctgcggggtg ctgctgccca actttcttgt
1201acaaagtggt tggtaagcct atccctaacc ctctcctcgg tctcgattct acgtagtaat
1261gagctagcgc taaccggtgg cgcgttaagt cgacaatcaa cctctggatt a

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