Transcript: Human NM_017556.4

Homo sapiens filamin binding LIM protein 1 (FBLIM1), transcript variant 1, mRNA.

Source:
NCBI, updated 2019-07-06
Taxon:
Homo sapiens (human)
Gene:
FBLIM1 (54751)
Length:
3314
CDS:
265..1386

Additional Resources:

NCBI RefSeq record:
NM_017556.4
NBCI Gene record:
FBLIM1 (54751)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_017556.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000149253 GAACAACCATCTCTTCTGCAA pLKO.1 1326 CDS 100% 2.640 3.696 N FBLIM1 n/a
2 TRCN0000319270 GAACAACCATCTCTTCTGCAA pLKO_005 1326 CDS 100% 2.640 3.696 N FBLIM1 n/a
3 TRCN0000148836 CTCACTCATTGCAGACTTAGA pLKO.1 645 CDS 100% 4.950 3.465 N FBLIM1 n/a
4 TRCN0000319336 CTCACTCATTGCAGACTTAGA pLKO_005 645 CDS 100% 4.950 3.465 N FBLIM1 n/a
5 TRCN0000149805 GCACGGACAAGTTAAACAGAA pLKO.1 2947 3UTR 100% 4.950 3.465 N FBLIM1 n/a
6 TRCN0000319339 GCACGGACAAGTTAAACAGAA pLKO_005 2947 3UTR 100% 4.950 3.465 N FBLIM1 n/a
7 TRCN0000146787 CCTTTGAAGGAGAATCATCAT pLKO.1 2893 3UTR 100% 4.950 2.970 N FBLIM1 n/a
8 TRCN0000319338 CCTTTGAAGGAGAATCATCAT pLKO_005 2893 3UTR 100% 4.950 2.970 N FBLIM1 n/a
9 TRCN0000166201 CATGGTGAAACCCTGTCTCTA pLKO.1 1874 3UTR 100% 4.950 2.475 Y ORAI2 n/a
10 TRCN0000127601 CGCTTGTAATCCCAGCACTTT pLKO.1 1796 3UTR 100% 4.950 2.475 Y CCDC30 n/a
11 TRCN0000140719 GATCACTTGAGGTCAGGAGTT pLKO.1 2136 3UTR 100% 4.050 2.025 Y P3H4 n/a
12 TRCN0000165299 GATCACTTGAGGTCAGGAGTT pLKO.1 2136 3UTR 100% 4.050 2.025 Y ORAI2 n/a
13 TRCN0000352971 GATCACTTGAGGTCAGGAGTT pLKO_005 2136 3UTR 100% 4.050 2.025 Y P3H4 n/a
14 TRCN0000078113 GCCTGTAATCCCAGCACTTTA pLKO.1 2098 3UTR 100% 13.200 6.600 Y LIAS n/a
15 TRCN0000179120 CAACATGGTGAAACCCTGTTT pLKO.1 1871 3UTR 100% 4.950 2.475 Y LOC339059 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_017556.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_08407 pDONR223 100% 99.9% 99.7% None 572C>T n/a
2 ccsbBroad304_08407 pLX_304 0% 99.9% 99.7% V5 572C>T n/a
3 TRCN0000475123 AGCGCATTTCAGTTTACTGAGTAC pLX_317 41.2% 99.9% 99.7% V5 572C>T n/a
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