Construct: ORF ccsbBroad304_08413
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000106.1_s304c1, BRDN0000400514
- Derived from:
- ccsbBroadEn_08413
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ZCCHC10 (54819)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_08413
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 54819 | ZCCHC10 | zinc finger CCHC-type conta... | NM_017665.4 | 100% | 100% | |
| 2 | human | 54819 | ZCCHC10 | zinc finger CCHC-type conta... | NM_001300819.3 | 91.7% | 91.7% | 199_200ins42 |
| 3 | human | 54819 | ZCCHC10 | zinc finger CCHC-type conta... | NM_001300816.3 | 88.5% | 88.5% | 42_107del |
| 4 | human | 54819 | ZCCHC10 | zinc finger CCHC-type conta... | NM_001300817.3 | 85.4% | 85.4% | 42_107del;309_310insAGGTCTAAGAGTGTAACC |
| 5 | human | 54819 | ZCCHC10 | zinc finger CCHC-type conta... | NM_001308127.2 | 84.1% | 84.1% | 42_137del |
| 6 | human | 54819 | ZCCHC10 | zinc finger CCHC-type conta... | NM_001300818.3 | 81.2% | 81.2% | 42_107del;265_266ins42 |
| 7 | human | 54819 | ZCCHC10 | zinc finger CCHC-type conta... | XM_011543502.1 | 77.2% | 77.2% | 42_137del;295_296ins42 |
| 8 | human | 54819 | ZCCHC10 | zinc finger CCHC-type conta... | NM_001308128.2 | 68.2% | 68.2% | 41_42ins162 |
| 9 | human | 54819 | ZCCHC10 | zinc finger CCHC-type conta... | NM_001308129.2 | 60% | 60% | 41_42ins204 |
| 10 | human | 54819 | ZCCHC10 | zinc finger CCHC-type conta... | NM_001308130.2 | 46% | 41.4% | 202_229del;276_277ins262 |
| 11 | human | 54819 | ZCCHC10 | zinc finger CCHC-type conta... | NM_001300822.3 | 41% | 36.8% | 42_107del;268_295del;342_343ins262 |
| 12 | human | 54819 | ZCCHC10 | zinc finger CCHC-type conta... | XM_011543503.2 | 39.1% | 35% | 42_137del;298_325del;372_373ins262 |
| 13 | human | 54819 | ZCCHC10 | zinc finger CCHC-type conta... | NR_125337.3 | 23.9% | 1_12del;54_57delGTAA;527_2127del | |
| 14 | human | 54819 | ZCCHC10 | zinc finger CCHC-type conta... | NR_131767.2 | 23.6% | (many diffs) | |
| 15 | human | 54819 | ZCCHC10 | zinc finger CCHC-type conta... | NR_131766.2 | 22.4% | (many diffs) | |
| 16 | mouse | 67966 | Zcchc10 | zinc finger, CCHC domain co... | NM_026479.4 | 79.1% | 83.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 576
- ORF length:
- 510
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggc gactcccatg catcggctaa tagcccggag acaagctgaa gcaaataagc 121 aacatgtaag atgtcagaaa tgcttggaat ttggacattg gacttatgaa tgcacaggaa 181 aaagaaaata cctacatagg ccctcaagga cagcagaact aaagaaagct ttaaaagaaa 241 aagaaaacag attattattg caacaaagca ttggagaaac caatgtagaa agaaaggcca 301 agaaaaaaag gtctaagagt gtaaccagtt ccagtagcag tagcagtgac agttctgcca 361 gtgattcttc atcagagagt gaagaaacat ctacctcttc ctcctcagag gacagtgaca 421 ctgatgaaag ctcctctagt tcctcatcct cagcctcctc cacaacctct tcctcctcct 481 ctgattcaga ctcagattcc agctcttcca gtagcagtag caccagcaca gatagcagct 541 ctgacgatga accaccaaag aagaagaaaa agaaataccc aactttcttg tacaaagtgg 601 ttggtaagcc tatccctaac cctctcctcg gtctcgattc tacgtagtaa tgagctagcg 661 ctaaccggtg gcgcgttaag tcgacaatca acctctggat ta