Construct: ORF ccsbBroad304_08421
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016321.1_s304c1, BRDN0000409449
- Derived from:
- ccsbBroadEn_08421
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TENT5C (54855)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_08421
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 54855 | TENT5C | terminal nucleotidyltransfe... | NM_017709.4 | 99.9% | 100% | 201C>T |
| 2 | mouse | 74645 | Fam46c | family with sequence simila... | NM_001142952.1 | 89.9% | 95.9% | (many diffs) |
| 3 | mouse | 74645 | Fam46c | family with sequence simila... | XM_006502212.3 | 89.9% | 95.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1239
- ORF length:
- 1173
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggc agaggagagc agctgtacca gggattgcat gtccttcagc gtgctcaact 121 gggatcaggt tagccggctg catgaggtcc tcactgaagt tgtacctatc cacggacgag 181 gcaactttcc aaccttggag ataactctga aggacatcgt ccagaccgtc cgcagtcggc 241 tggaggaggc aggcatcaaa gtgcatgacg tccggctgaa tggctccgca gctggccacg 301 ttttggtcaa agacaatggc ttgggctgca aagacctgga cctaatcttc catgtggctc 361 ttccaacaga ggcagaattt cagctggtta gagatgtggt tctgtgttcc cttctgaact 421 tcctgccaga gggtgtgaac aagctcaaaa tcagtccagt cactctgaag gaggcatatg 481 tgcagaagct agtgaaggtt tgcacggaca ctgaccgctg gagcctgatc tccctctcca 541 acaagaacgg gaagaacgtg gagctgaagt ttgtcgactc cattcggcgt cagtttgagt 601 tcagtgtgga ctctttccaa atcatcctgg attctttgct tttcttctat gactgttcca 661 ataatcccat ctctgagcac ttccacccca ccgtgattgg ggagagcatg tacggggact 721 ttgaggaagc ttttgaccat ctgcagaaca gactgatcgc caccaagaac ccagaagaaa 781 tcagaggcgg gggacttctc aagtacagca accttcttgt gcgggacttc aggcccacag 841 accaggaaga aatcaaaact ctagagcgct acatgtgctc caggttcttc atcgacttcc 901 cggacatcct tgaacagcag aggaagttgg agacttacct tcaaaaccac ttcgctgaag 961 aagagagaag caagtacgac tacctcatga tccttcgcag ggtggtgaac gagagcaccg 1021 tgtgtctcat ggggcatgaa cgcaggcaga ctctgaacct catctccctc ctggccttgc 1081 gtgtgctggc ggaacaaaac atcatcccca gtgccaccaa cgtcacctgt tactaccagc 1141 cggcccctta cgtcagtgat ggcaacttca gcaactacta cgttgcccat cctccagtca 1201 cctacagcca gccttaccct acctggctgc cctgtaacta cccaactttc ttgtacaaag 1261 tggttggtaa gcctatccct aaccctctcc tcggtctcga ttctacgtag taatgagcta 1321 gcgctaaccg gtggcgcgtt aagtcgacaa tcaacctctg gatta