Construct: ORF ccsbBroad304_08554

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF011515.1_s304c1, BRDN0000404693
Derived from:
ccsbBroadEn_08554
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
TTC27 (55622)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_08554
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 55622 TTC27 tetratricopeptide repeat do... NM_017735.5 99.9% 100% 114A>G;1620C>T
2 human 55622 TTC27 tetratricopeptide repeat do... XM_011532958.2 96% 96% 114A>G;1620C>T;1680_1681ins99
3 human 55622 TTC27 tetratricopeptide repeat do... XM_005264416.2 95% 95.1% 114A>G;1327_1328ins123;1497C>T
4 human 55622 TTC27 tetratricopeptide repeat do... NM_001193509.2 94% 94% 0_1ins150;1470C>T
5 human 55622 TTC27 tetratricopeptide repeat do... XR_002959314.1 76.3% (many diffs)
6 mouse 74196 Ttc27 tetratricopeptide repeat do... XM_006525027.3 48% 49.5% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
2595
ORF length:
2529
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgtg gaccccggag ctggcaattc tgaggggatt ccccactgag gctgagcggc
121agcaatggaa acaggagggg gtcgtcggtt cagagagtgg atctttccta caattgctgc
181tggaagggaa ctatgaagcc atattcttaa attcaatgac tcaaaatatt tttaattcaa
241caacaaccgc tgaagaaaag attgatagct acctggagaa gcaggtagta acattcctgg
301attactcaac agatttggac acaacggaaa gacaacagtt gatatttcta cttggtgtga
361gcagtttgca actttttgtt cagagcaact ggacggggcc ccctgttgac ttacaccctc
421aggacttttt gtcatctgtt ttgttccagc aattcagtga ggttaaagga ctggatgcat
481ttgttctgag cctgctcact ctagatggtg aatcaatcta cagcctgacc tcgaagccta
541tactactgtt attagcacgc attatcctag tgaatgtaag acataaactg acagctattc
601agagcttgcc atggtggact ttgagatgtg tgaatattca tcagcatttg cttgaggaac
661gctcacctct gctttttact cttgccgaaa actgtattga tcaagtgatg aaactacaga
721atctgtttgt agatgattca ggtcgatatt tggctattca attccatctg gaatgtgcat
781atgtgttttt atattattat gagtacagaa aagcaaaaga tcagttggat attgctaagg
841acatcagcca attacaaatt gatttgacag gtgctttggg aaaaagaaca cggttccagg
901aaaattatgt ggcacaactg attctagatg taagaaggga aggggatgtc ctttcaaatt
961gtgaattcac tccagcaccc actcctcagg aacatttaac caagaatctt gagctcaatg
1021atgacaccat tctgaatgac ataaagttag cagattgtga acagttccag atgccggatc
1081tgtgtgctga agagatcgct attattcttg gaatctgcac taattttcaa aagaataacc
1141cagtgcacac attaactgaa gtggagcttc tggcatttac atcatgtttg ctttcacaac
1201caaagttctg ggccattcag acatcagcct tgatcctccg gacaaaactt gagaaaggaa
1261gtactcgccg agtggaacgg gcaatgaggc agacacaggc tcttgcagac caatttgaag
1321ataaaactac atctgtattg gaacgcctga agattttcta ttgctgtcaa gtaccacctc
1381actgggccat tcagcgccaa cttgcaagtt tgctctttga gttgggatgt accagttcag
1441cccttcagat atttgaaaag ctagaaatgt gggaagatgt tgtcatttgt tatgaaagag
1501ccgggcagca cggaaaggca gaagaaatcc ttagacaaga gctggagaaa aaagaaacgc
1561ctagtttata ctgcttgctt ggagatgtcc tcggagacca ttcttgctat gacaaggcct
1621gggagttgtc ccggtaccgc agtgctcgtg ctcagcgctc caaagccctc cttcatcttc
1681ggaataagga gtttcaagag tgtgtagagt gcttcgaacg ctcggttaag attaatccca
1741tgcagctcgg ggtgtggttt tctctcggtt gtgcctattt ggccttggaa gactatcaag
1801gttcagcaaa ggcatttcag cgctgtgtga ctctagaacc cgataatgct gaagcttgga
1861acaatttgtc aacttcctat atccgattaa aacaaaaagt aaaagctttt agaactttac
1921aagaagctct caagtgtaac tatgaacact ggcagatttg ggaaaactac atcctcacca
1981gcactgacgt tggggaattt tcagaagcca ttaaagctta tcaccggctc ttggacttac
2041gtgacaaata caaagatgtt caggtcctta aaattctagt cagggcagtg attgatggga
2101tgactgatcg aagtggagat gttgcaactg gcctcaaagg aaagctgcag gagttatttg
2161gcagagtgac ttcaagagtg acaaatgatg gagaaatctg gaggctgtat gcccacgtat
2221atggaaatgg gcagagtgaa aagcctgatg aaaatgaaaa ggcattccag tgcctctcaa
2281aggcatacaa gtgtgacacc cagtccaatt gttgggagaa agatattaca tcatttaagg
2341aagttgttca aagagcctta ggacttgcac atgtggccat aaaatgcagt aaaaacaaat
2401ccagttccca agaagctgta caaatgcttt cttctgttcg actcaattta cggggcttgt
2461tatctaaagc aaagcaactt tttacagatg tggcaactgg agaaatgtcc agggaattag
2521ctgatgacat aacagctatg gacaccttag tgacagagct ccaagaccta agcaaccagt
2581ttcgaaatca gtattgccca actttcttgt acaaagtggt tggtaagcct atccctaacc
2641ctctcctcgg tctcgattct acgtagtaat gagctagcgc taaccggtgg cgcgttaagt
2701cgacaatcaa cctctggatt a

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