Construct: ORF ccsbBroad304_08554
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011515.1_s304c1, BRDN0000404693
- Derived from:
- ccsbBroadEn_08554
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TTC27 (55622)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_08554
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55622 | TTC27 | tetratricopeptide repeat do... | NM_017735.5 | 99.9% | 100% | 114A>G;1620C>T |
| 2 | human | 55622 | TTC27 | tetratricopeptide repeat do... | XM_011532958.2 | 96% | 96% | 114A>G;1620C>T;1680_1681ins99 |
| 3 | human | 55622 | TTC27 | tetratricopeptide repeat do... | XM_005264416.2 | 95% | 95.1% | 114A>G;1327_1328ins123;1497C>T |
| 4 | human | 55622 | TTC27 | tetratricopeptide repeat do... | NM_001193509.2 | 94% | 94% | 0_1ins150;1470C>T |
| 5 | human | 55622 | TTC27 | tetratricopeptide repeat do... | XR_002959314.1 | 76.3% | (many diffs) | |
| 6 | mouse | 74196 | Ttc27 | tetratricopeptide repeat do... | XM_006525027.3 | 48% | 49.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2595
- ORF length:
- 2529
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgtg gaccccggag ctggcaattc tgaggggatt ccccactgag gctgagcggc 121 agcaatggaa acaggagggg gtcgtcggtt cagagagtgg atctttccta caattgctgc 181 tggaagggaa ctatgaagcc atattcttaa attcaatgac tcaaaatatt tttaattcaa 241 caacaaccgc tgaagaaaag attgatagct acctggagaa gcaggtagta acattcctgg 301 attactcaac agatttggac acaacggaaa gacaacagtt gatatttcta cttggtgtga 361 gcagtttgca actttttgtt cagagcaact ggacggggcc ccctgttgac ttacaccctc 421 aggacttttt gtcatctgtt ttgttccagc aattcagtga ggttaaagga ctggatgcat 481 ttgttctgag cctgctcact ctagatggtg aatcaatcta cagcctgacc tcgaagccta 541 tactactgtt attagcacgc attatcctag tgaatgtaag acataaactg acagctattc 601 agagcttgcc atggtggact ttgagatgtg tgaatattca tcagcatttg cttgaggaac 661 gctcacctct gctttttact cttgccgaaa actgtattga tcaagtgatg aaactacaga 721 atctgtttgt agatgattca ggtcgatatt tggctattca attccatctg gaatgtgcat 781 atgtgttttt atattattat gagtacagaa aagcaaaaga tcagttggat attgctaagg 841 acatcagcca attacaaatt gatttgacag gtgctttggg aaaaagaaca cggttccagg 901 aaaattatgt ggcacaactg attctagatg taagaaggga aggggatgtc ctttcaaatt 961 gtgaattcac tccagcaccc actcctcagg aacatttaac caagaatctt gagctcaatg 1021 atgacaccat tctgaatgac ataaagttag cagattgtga acagttccag atgccggatc 1081 tgtgtgctga agagatcgct attattcttg gaatctgcac taattttcaa aagaataacc 1141 cagtgcacac attaactgaa gtggagcttc tggcatttac atcatgtttg ctttcacaac 1201 caaagttctg ggccattcag acatcagcct tgatcctccg gacaaaactt gagaaaggaa 1261 gtactcgccg agtggaacgg gcaatgaggc agacacaggc tcttgcagac caatttgaag 1321 ataaaactac atctgtattg gaacgcctga agattttcta ttgctgtcaa gtaccacctc 1381 actgggccat tcagcgccaa cttgcaagtt tgctctttga gttgggatgt accagttcag 1441 cccttcagat atttgaaaag ctagaaatgt gggaagatgt tgtcatttgt tatgaaagag 1501 ccgggcagca cggaaaggca gaagaaatcc ttagacaaga gctggagaaa aaagaaacgc 1561 ctagtttata ctgcttgctt ggagatgtcc tcggagacca ttcttgctat gacaaggcct 1621 gggagttgtc ccggtaccgc agtgctcgtg ctcagcgctc caaagccctc cttcatcttc 1681 ggaataagga gtttcaagag tgtgtagagt gcttcgaacg ctcggttaag attaatccca 1741 tgcagctcgg ggtgtggttt tctctcggtt gtgcctattt ggccttggaa gactatcaag 1801 gttcagcaaa ggcatttcag cgctgtgtga ctctagaacc cgataatgct gaagcttgga 1861 acaatttgtc aacttcctat atccgattaa aacaaaaagt aaaagctttt agaactttac 1921 aagaagctct caagtgtaac tatgaacact ggcagatttg ggaaaactac atcctcacca 1981 gcactgacgt tggggaattt tcagaagcca ttaaagctta tcaccggctc ttggacttac 2041 gtgacaaata caaagatgtt caggtcctta aaattctagt cagggcagtg attgatggga 2101 tgactgatcg aagtggagat gttgcaactg gcctcaaagg aaagctgcag gagttatttg 2161 gcagagtgac ttcaagagtg acaaatgatg gagaaatctg gaggctgtat gcccacgtat 2221 atggaaatgg gcagagtgaa aagcctgatg aaaatgaaaa ggcattccag tgcctctcaa 2281 aggcatacaa gtgtgacacc cagtccaatt gttgggagaa agatattaca tcatttaagg 2341 aagttgttca aagagcctta ggacttgcac atgtggccat aaaatgcagt aaaaacaaat 2401 ccagttccca agaagctgta caaatgcttt cttctgttcg actcaattta cggggcttgt 2461 tatctaaagc aaagcaactt tttacagatg tggcaactgg agaaatgtcc agggaattag 2521 ctgatgacat aacagctatg gacaccttag tgacagagct ccaagaccta agcaaccagt 2581 ttcgaaatca gtattgccca actttcttgt acaaagtggt tggtaagcct atccctaacc 2641 ctctcctcgg tctcgattct acgtagtaat gagctagcgc taaccggtgg cgcgttaagt 2701 cgacaatcaa cctctggatt a