Construct: ORF ccsbBroad304_08615
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016340.1_s304c1, BRDN0000402538
- Derived from:
- ccsbBroadEn_08615
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GNG12 (55970)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_08615
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55970 | GNG12 | G protein subunit gamma 12 | NM_018841.6 | 99.5% | 98.6% | 85A>G |
| 2 | human | 55970 | GNG12 | G protein subunit gamma 12 | XM_017001809.2 | 99.5% | 98.6% | 85A>G |
| 3 | human | 55970 | GNG12 | G protein subunit gamma 12 | XM_024448405.1 | 99.5% | 98.6% | 85A>G |
| 4 | mouse | 14701 | Gng12 | guanine nucleotide binding ... | NM_001177556.1 | 86.1% | 95.8% | (many diffs) |
| 5 | mouse | 14701 | Gng12 | guanine nucleotide binding ... | NM_001177557.1 | 86.1% | 95.8% | (many diffs) |
| 6 | mouse | 14701 | Gng12 | guanine nucleotide binding ... | NM_001177558.1 | 86.1% | 95.8% | (many diffs) |
| 7 | mouse | 14701 | Gng12 | guanine nucleotide binding ... | NM_001177559.1 | 86.1% | 95.8% | (many diffs) |
| 8 | mouse | 14701 | Gng12 | guanine nucleotide binding ... | NM_001177560.1 | 86.1% | 95.8% | (many diffs) |
| 9 | mouse | 14701 | Gng12 | guanine nucleotide binding ... | NM_025278.5 | 86.1% | 95.8% | (many diffs) |
| 10 | mouse | 14701 | Gng12 | guanine nucleotide binding ... | XM_006505542.3 | 86.1% | 95.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 282
- ORF length:
- 216
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgtc cagcaaaaca gcaagcacca acaatatagc ccaggcaagg agaactgtgc 121 agcagttaag attagaagcc tccattgaag gaataaaggt ttcgaaggca tcagcggacc 181 tcatgtccta ctgtgaggaa catgccagga gtgacccttt gctgatagga ataccaactt 241 cagaaaaccc tttcaaggat aaaaaaactt gcatcatctt atacccaact ttcttgtaca 301 aagtggttgg taagcctatc cctaaccctc tcctcggtct cgattctacg tagtaatgag 361 ctagcgctaa ccggtggcgc gttaagtcga caatcaacct ctggatta