Construct: ORF ccsbBroad304_08721

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF017688.1_s304c1, BRDN0000400323
Derived from:
ccsbBroadEn_08721
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
JPH3 (57338)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_08721
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 57338 JPH3 junctophilin 3 NM_020655.4 99.8% 99.5% 1127T>C;1414C>A;1934C>T
2 human 57338 JPH3 junctophilin 3 NR_073379.2 69.8% (many diffs)
3 human 57338 JPH3 junctophilin 3 NM_001271604.3 22.4% 17.6% (many diffs)
4 human 57338 JPH3 junctophilin 3 NM_001271605.2 18.8% 17.4% (many diffs)
5 mouse 57340 Jph3 junctophilin 3 NM_020605.3 87.8% 90.3% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
2310
ORF length:
2244
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgtc cagtgggggc aggtttaatt ttgacgacgg agggtcctac tgtggaggct
121gggaggacgg caaggcgcac ggccatggcg tctgcaccgg ccccaagggc caaggcgaat
181acaccggctc gtggagccac ggcttcgagg tgctgggcgt ctacacctgg cccagcggca
241acacgtacca gggcacctgg gcgcagggca agcgccacgg catcggcctg gagagcaagg
301ggaagtgggt gtacaagggc gagtggacgc acggattcaa ggggcgctac ggggtgcggg
361agtgcgcggg caacggggcc aaatacgaag ggacctggag caacgggctg caggacggct
421acgggaccga gacctactcg gacggaggga cctaccaggg ccagtgggtc ggtggcatgc
481gccagggcta cggcgtccgg cagagcgtcc cgtatggcat ggccgcggtc atccgctcac
541ccctgaggac gtccatcaac tccctgcgca gcgagcacac caacggcacg gcgctgcatc
601ccgacgcctc tccggcggtg gccggcagcc cggccgtgtc ccgcgggggc ttcgtgctcg
661tggcccacag tgactccgag atcctcaaga gcaagaagaa ggggctgttt cggcgctcgc
721tgctgagtgg gctgaagctg cgcaagtcgg agtccaagag cagcctggcc agccaacgca
781gcaagcagag ctcctttcgc agcgaggcgg gcatgagcac cgtcagctcc acggccagcg
841acatccactc caccatcagc ctgggcgagg ctgaggccga gctggcggtc atcgaggacg
901acatcgacgc caccaccacc gagacctacg tgggcgagtg gaagaacgac aaacgctccg
961gcttcggcgt gagccagcgc tcggacgggc tcaagtacga gggcgagtgg gccagcaacc
1021ggcgccatgg ctacggctgc atgaccttcc cggacggcac caaggaggag ggcaagtaca
1081agcagaacat cctcgtcggc ggcaagcgca agaacctcat ccccctgcgg gccagcaaga
1141tccgcgagaa ggtggaccgc gccgttgagg ccgctgagcg ggccgccacc accgccaagc
1201agaaggctga gatcgcggct tccaggacct cccactctcg ggcaaaggcc gaggcagccc
1261tcacagcagc tcagaaagcc caggaggagg cgcggatcgc caggatcact gccaaagagt
1321tctccccttc cttccagcac cgggaaaacg ggctggagta ccagaggccg aagcgtcaga
1381cctcctgtga cgacatcgag gtgctgtcca ccgggacacc cctgcagcag gagagccccg
1441agctgtaccg caagggcacc actccctccg acctgaccac cgacgacagc cccctgcaga
1501gcttccccac cagccccgcg gccaccccgc cgcccgcgcc cgccgccagg aacaaggtcg
1561cccacttctc gaggcaggtg tcggtggacg aggagcgggg cggggacatc cagatgctcc
1621tggagggccg ggccggggac tgcgcccgca gcagctgggg cgaggagcag gccgggggct
1681ccaggggtgt ccgcagcggt gccctgcgcg gcggcctgct cgtggatgac ttccgcaccc
1741gaggttcggg ccgcaagcag cccgggaacc ccaagccgcg ggagcggcgg acggagtcac
1801cccccgtgtt cacgtggact tcccaccacc gggccagcaa ccacagcccc ggaggctcca
1861ggctgctgga gctgcaggag gagaagctga gcaactaccg gatggagatg aaacccttgc
1921tgaggatgga gacgcatccc cagaaaagac gctacagcaa gggcggcgcc tgccggggct
1981tgggggacga ccaccgcctc gaggaccggg gcttcggggt gcagagactg cggtccaagg
2041cccagaacaa ggagaacttc aggccggcct cctccgcgga gcccgccgtg cagaaactgg
2101cgagcctgcg gctgggcggg gccgagcccc ggttgctgcg ttgggacttg accttctccc
2161cgccccagaa atccttgcct gtcgctctag agtccgacga ggagaatggg gatgagctca
2221agtccagtac gggctcagcg cctatcctgg tggtcatggt gatcttgctc aacatcggag
2281tcgccattct gtttattaac tttttcatct gcccaacttt cttgtacaaa gtggttggta
2341agcctatccc taaccctctc ctcggtctcg attctacgta gtaatgagct agcgctaacc
2401ggtggcgcgt taagtcgaca atcaacctct ggatta

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