Construct: ORF ccsbBroad304_08745

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF009951.1_s304c1, BRDN0000401829
Derived from:
ccsbBroadEn_08745
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
PCDH10 (57575)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_08745
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 57575 PCDH10 protocadherin 10 NM_032961.3 99.9% 99.9% 1214T>C
2 human 57575 PCDH10 protocadherin 10 XM_011532150.2 98.1% 97.8% (many diffs)
3 human 57575 PCDH10 protocadherin 10 NM_020815.3 85.3% 84.6% (many diffs)
4 mouse 18526 Pcdh10 protocadherin 10 NM_001098171.1 89.7% 97.3% (many diffs)
5 mouse 18526 Pcdh10 protocadherin 10 NM_001098172.1 88.5% 96.1% (many diffs)
6 mouse 18526 Pcdh10 protocadherin 10 NM_001098170.1 87.8% 95% (many diffs)
7 mouse 18526 Pcdh10 protocadherin 10 NM_011043.3 75.8% 82% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
3189
ORF length:
3120
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcaccat gattgtgcta ttattgtttg ccttgctctg gatggtggaa ggagtctttt
121cccagcttca ctacacggta caggaggagc aggaacatgg cactttcgtg gggaatatcg
181ctgaagatct gggtctggac attacaaaac tttcggctcg cgggtttcag acggtgccca
241actcaaggac cccttactta gacctcaacc tggagacagg ggtgctgtac gtgaacgaga
301aaatagaccg cgaacaaatc tgcaaacaga gcccctcctg tgtcctgcac ctggaggtct
361ttctggagaa ccccctggag ctgttccagg tggagatcga ggtgctggac attaatgaca
421accccccctc tttcccggag ccagacctga cggtggaaat ctctgagagc gccacgccag
481gcactcgctt ccccttggag agcgcattcg acccagacgt gggcaccaac tccttgcgcg
541actacgagat cacccccaac agctacttct ccctggacgt gcagacccag ggggatggca
601accgattcgc tgagctggtg ctggagaagc cactggaccg agagcagcaa gcggtgcacc
661gctacgtgct gaccgcggtg gacggaggag gtgggggagg agtaggagaa ggagggggag
721gtggcggggg agcaggcctg cccccccagc agcagcgcac cggcacggcc ctactcacca
781tccgagtgct ggactccaat gacaatgtgc ccgctttcga ccaacccgtc tacactgtgt
841ccctaccaga gaactctccc ccaggcactc tcgtgatcca gctcaacgcc accgacccgg
901acgagggcca gaacggtgag gtcgtgtact ccttcagcag ccacatttcg ccccgggcgc
961gggagctttt cggactctcg ccgcgcactg gcagactgga ggtaagcggc gagttggact
1021atgaagagag cccagtgtac caagtgtacg tgcaagccaa ggacctgggc cccaacgccg
1081tgcctgcgca ctgcaaggtg ctagtgcgag tactggatgc taatgacaac gcgccagaga
1141tcagcttcag caccgtgaag gaagcggtga gtgagggcgc ggcgcccggc actgtggtgg
1201cccttttcag cgtgactgac cgcgactcag aggagaatgg gcaggtgcag tgcgagctac
1261tgggagacgt gcctttccgc cccaagtctt cctttaagaa ttactacacc atcgttaccg
1321aagcccccct ggaccgagag gcgggggact cctacaccct gactgtagtg gctcgggacc
1381ggggcgagcc tgcgctctcc accagtaagt cgatccaggt acaagtgtcg gatgtgaacg
1441acaacgcgcc gcgtttcagc cagccggtct acgacgtgta tgtgactgaa aacaacgtgc
1501ctggcgccta catctacgcg gtgagcgcca ccgaccggga tgagggcgcc aacgcccagc
1561ttgcctactc tatcctcgag tgccagatcc agggcatgag cgtcttcacc tacgtttcta
1621tcaactctga gaacggctac ttgtacgccc tgcgctcctt cgactatgag cagctgaagg
1681acttcagttt tcaggtggaa gcccgggacg ctggcagccc ccaggcgctg gctggtaacg
1741ccactgtcaa catcctcata gtggatcaaa atgacaacgc ccctgccatc gtggcgcctc
1801taccagggcg caacgggact ccagcgcgtg aggtgctgcc ccgctcggcg gagccgggtt
1861acctgctcac ccgcgtggcc gccgtggacg cggacgacgg cgagaacgcc cggctcactt
1921acagcatcgt gcgtggcaac gaaatgaacc tctttcgcat ggactggcgc accggggagc
1981tgcgcacagc acgccgagtc ccggccaagc gcgaccccca gcggccttat gagctggtga
2041tcgaggtgcg cgaccatggg cagccgcccc tttcctccac cgccaccctg gtggttcagc
2101tggtggatgg cgccgtggag ccccagggcg ggggcgggag cggaggcgga gggtcaggag
2161agcaccagcg ccccagtcgc tctggcggcg gggaaacctc gctagacctc accctcatcc
2221tcatcatcgc gttgggctcg gtgtccttca tcttcctgct ggccatgatc gtgctggccg
2281tgcgttgcca aaaagagaag aagctcaaca tctatacttg tctggccagc gattgctgcc
2341tctgctgctg ctgctgcggt ggcggaggtt cgacctgctg tggccgccaa gcccgggcgc
2401gcaagaagaa actcagcaag tcagacatca tgctggtgca gagctccaat gtacccagta
2461acccggccca ggtgccgata gaggagtccg ggggctttgg ctcccaccac cacaaccaga
2521attactgcta tcaggtatgc ctgacccctg agtccgccaa gaccgacctg atgtttctta
2581agccctgcag cccttcgcgg agtacggaca ctgagcacaa cccctgcggg gccatcgtca
2641ccggttacac cgaccagcag cctgatatca tctccaacgg aagcattttg tccaacgaga
2701ctaaacacca gcgagcagag ctcagctatc tagttgacag acctcgccga gttaacagtt
2761ctgcattcca ggaagccgac atagtaagct ctaaggacag tggtcatgga gacagtgaac
2821agggagatag tgatcatgat gccaccaacc gtgcccagtc agctggtatg gatctcttct
2881ccaattgcac tgaggaatgt aaagctctgg gccactcaga tcggtgctgg atgccttctt
2941ttgtcccttc tgatggacgc caggctgctg attatcgcag caatctgcat gttcctggca
3001tggactctgt tccagacact gaggtgtttg aaactccaga agcccagcct ggggcagagc
3061ggtccttttc cacctttggc aaagagaagg cccttcacag cactctggag aggaaggagc
3121tggatggact gctgactaat acgcgagcgc cttacaaacc accatatttg acacggaaaa
3181ggatatgctt gccaactttc ttgtacaaag tggttggtaa gcctatccct aaccctctcc
3241tcggtctcga ttctacgtag taatgagcta gcgctaaccg gtggcgcgtt aagtcgacaa
3301tcaacctctg gatta

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