Construct: ORF ccsbBroad304_08778
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001250.1_s304c1, BRDN0000398799
- Derived from:
- ccsbBroadEn_08778
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RPRD1B (58490)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_08778
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 58490 | RPRD1B | regulation of nuclear pre-m... | NM_021215.4 | 99.8% | 99.6% | 63G>C |
| 2 | human | 58490 | RPRD1B | regulation of nuclear pre-m... | XM_011528954.3 | 89.5% | 79.8% | (many diffs) |
| 3 | human | 58490 | RPRD1B | regulation of nuclear pre-m... | XM_017027990.2 | 89.5% | 79.8% | (many diffs) |
| 4 | human | 58490 | RPRD1B | regulation of nuclear pre-m... | XM_005260477.4 | 85.4% | 84.6% | (many diffs) |
| 5 | human | 58490 | RPRD1B | regulation of nuclear pre-m... | XM_017027991.1 | 66.8% | 59.1% | 0_1ins190;89_90ins134 |
| 6 | human | 58490 | RPRD1B | regulation of nuclear pre-m... | XM_005260480.5 | 58.7% | 58.2% | (many diffs) |
| 7 | human | 58490 | RPRD1B | regulation of nuclear pre-m... | XR_936597.3 | 49.8% | (many diffs) | |
| 8 | human | 58490 | RPRD1B | regulation of nuclear pre-m... | XR_001754361.1 | 40.7% | (many diffs) | |
| 9 | mouse | 70470 | Rprd1b | regulation of nuclear pre-m... | NM_001291134.1 | 92.2% | 99% | (many diffs) |
| 10 | mouse | 70470 | Rprd1b | regulation of nuclear pre-m... | NM_027434.3 | 92% | 99% | (many diffs) |
| 11 | mouse | 70470 | Rprd1b | regulation of nuclear pre-m... | NM_001291135.1 | 82.4% | 84.6% | (many diffs) |
| 12 | mouse | 70470 | Rprd1b | regulation of nuclear pre-m... | XM_006500162.1 | 82.2% | 84.6% | (many diffs) |
| 13 | mouse | 70470 | Rprd1b | regulation of nuclear pre-m... | XM_006500163.1 | 62.5% | 66.2% | (many diffs) |
| 14 | mouse | 70470 | Rprd1b | regulation of nuclear pre-m... | XM_011239773.1 | 54.6% | 60.1% | (many diffs) |
| 15 | mouse | 70470 | Rprd1b | regulation of nuclear pre-m... | NM_001291136.1 | 54.3% | 60.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1044
- ORF length:
- 978
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgtc ctccttctct gagtcggcgc tggagaagaa gctctcggag ctgagcaact 121 ctcagcacag cgtgcagacc ctgtcccttt ggctcatcca ccaccgcaag cacgcgggac 181 ccatcgtctc cgtgtggcac cgcgagctcc gcaaagccaa atcaaataga aagcttactt 241 ttctgtattt agcgaatgat gtcatccaaa acagtaaaag gaaaggacct gaattcacta 301 gagaatttga atctgtcctt gtggatgctt tttctcatgt tgccagagag gcagatgaag 361 gctgtaaaaa acctttagaa agattgctga acatctggca agaacgaagt gtgtatggcg 421 gcgagttcat acagcagctg aagctgtcta tggaggactc caagagccct ccccccaaag 481 caacagaaga gaagaaatct ctgaaacgaa cttttcagca aattcaggag gaggaggatg 541 acgactaccc tggcagctac tctcctcagg atccttctgc aggacccctc ttgactgagg 601 aactaatcaa agctttgcag gatctggaaa atgccgcatc aggggatgct actgtccgac 661 agaaaattgc ttctctgccc caggaagtgc aagatgtttc tctattggaa aaaataacag 721 acaaagaggc agctgaacgt ctttcaaaaa cagtagatga agcatgtctg ttactagcag 781 aatataacgg gcgcctggca gcagaactgg aggaccgtcg ccagctggct cggatgttgg 841 tggagtatac ccagaatcag aaagatgttt tgtcggagaa ggagaaaaaa ctagaggaat 901 acaaacagaa gcttgcacga gtaacccagg tccgcaagga actgaaatcc catattcaga 961 gcttgccaga cctctcactg ctgcccaacg tcacaggggg cttagccccc ctgccctctg 1021 ctggggacct gttttcaact gactacccaa ctttcttgta caaagtggtt ggtaagccta 1081 tccctaaccc tctcctcggt ctcgattcta cgtagtaatg agctagcgct aaccggtggc 1141 gcgttaagtc gacaatcaac ctctggatta