Transcript: Human XM_005260480.5

PREDICTED: Homo sapiens regulation of nuclear pre-mRNA domain containing 1B (RPRD1B), transcript variant X7, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
RPRD1B (58490)
Length:
3821
CDS:
749..1330

Additional Resources:

NCBI RefSeq record:
XM_005260480.5
NBCI Gene record:
RPRD1B (58490)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_005260480.5, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000149087 GCACGAAGATTAGGTGCATTT pLKO.1 1896 3UTR 100% 10.800 15.120 N RPRD1B n/a
2 TRCN0000148172 GAGGAATACAAACAGAAGCTT pLKO.1 1178 CDS 100% 3.000 4.200 N RPRD1B n/a
3 TRCN0000130654 CCTCTTGACTGAGGAACTAAT pLKO.1 871 CDS 100% 13.200 9.240 N RPRD1B n/a
4 TRCN0000352946 CCTCTTGACTGAGGAACTAAT pLKO_005 871 CDS 100% 13.200 9.240 N RPRD1B n/a
5 TRCN0000241221 CTCCGCAAAGCCAAATCAAAT pLKO_005 354 5UTR 100% 13.200 9.240 N Rprd1b n/a
6 TRCN0000129183 CAAGGAACTGAAATCCCATAT pLKO.1 1219 CDS 100% 10.800 7.560 N RPRD1B n/a
7 TRCN0000174988 GCAAAGCCAAATCAAATAGAA pLKO.1 358 5UTR 100% 5.625 3.938 N Rprd1b n/a
8 TRCN0000148141 GCCTCTTAATACCTGTTACTA pLKO.1 3373 3UTR 100% 5.625 3.938 N RPRD1B n/a
9 TRCN0000352948 GCCTCTTAATACCTGTTACTA pLKO_005 3373 3UTR 100% 5.625 3.938 N RPRD1B n/a
10 TRCN0000148750 CGGCAGCAGTATATTCTGAAA pLKO.1 3752 3UTR 100% 4.950 3.465 N RPRD1B n/a
11 TRCN0000130891 GCATGTCTGTTACTAGCAGAA pLKO.1 1046 CDS 100% 4.050 2.835 N RPRD1B n/a
12 TRCN0000352947 GCATGTCTGTTACTAGCAGAA pLKO_005 1046 CDS 100% 4.050 2.835 N RPRD1B n/a
13 TRCN0000129003 GCAAGATGTTTCTCTATTGGA pLKO.1 973 CDS 100% 3.000 2.100 N RPRD1B n/a
14 TRCN0000343643 GCAAGATGTTTCTCTATTGGA pLKO_005 973 CDS 100% 3.000 2.100 N RPRD1B n/a
15 TRCN0000174628 GCCAAATCAAATAGAAAGCTT pLKO.1 363 5UTR 100% 3.000 1.800 N Rprd1b n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_005260480.5, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_08778 pDONR223 100% 58.7% 58.2% None (many diffs) n/a
2 ccsbBroad304_08778 pLX_304 0% 58.7% 58.2% V5 (many diffs) n/a
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