Construct: ORF ccsbBroad304_08789
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008244.1_s304c1, BRDN0000409281
- Derived from:
- ccsbBroadEn_08789
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- AFAP1 (60312)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_08789
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 60312 | AFAP1 | actin filament associated p... | NM_001371090.1 | 99.8% | 99.7% | 297G>A;1208C>G;2176T>A |
| 2 | human | 60312 | AFAP1 | actin filament associated p... | NM_001371091.1 | 99.8% | 99.7% | 297G>A;1208C>G;2176T>A |
| 3 | human | 60312 | AFAP1 | actin filament associated p... | NM_198595.2 | 99.8% | 99.7% | 297G>A;1208C>G;2176T>A |
| 4 | human | 60312 | AFAP1 | actin filament associated p... | XM_017008535.1 | 97.4% | 97.3% | (many diffs) |
| 5 | human | 60312 | AFAP1 | actin filament associated p... | XM_006713909.3 | 97% | 96.9% | (many diffs) |
| 6 | human | 60312 | AFAP1 | actin filament associated p... | NM_001134647.1 | 89.5% | 89.4% | (many diffs) |
| 7 | human | 60312 | AFAP1 | actin filament associated p... | XM_011513544.3 | 89.5% | 89.4% | (many diffs) |
| 8 | human | 60312 | AFAP1 | actin filament associated p... | XM_017008536.1 | 69.9% | 71.3% | (many diffs) |
| 9 | mouse | 70292 | Afap1 | actin filament associated p... | NM_027373.2 | 84.3% | 91.3% | (many diffs) |
| 10 | mouse | 70292 | Afap1 | actin filament associated p... | XM_006504098.2 | 75.3% | 81.4% | (many diffs) |
| 11 | mouse | 70292 | Afap1 | actin filament associated p... | XM_006504100.3 | 63.9% | 68.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2256
- ORF length:
- 2190
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgga agagttaata gttgaacttc gtctctttct tgaactcctg gaccatgaat 121 atctaacctc aactgtcagg gagaaaaagg cagtgataac caacattctg ctaagaatac 181 agtcatccaa aggttttgat gtgaaggacc atgctcagaa gcaggagacc gctaacagcc 241 tgccagcccc tcctcagatg cccctgccgg agatccctca gccctggctg cctcctgaca 301 gtgggcctcc accattgcca acatcctccc tcccagaagg ttattatgag gaagctgtgc 361 cactgagccc cggaaaagct ccggaataca tcacatcaaa ttatgattcc gatgcgatga 421 gcagctctta tgagtcgtat gatgaagagg aggaggatgg gaaggggaag aaaacccggc 481 accagtggcc ctccgaggag gcctccatgg acctggtcaa ggacgccaaa atctgcgcct 541 tcctgctgcg gaagaagcgg ttcggccagt ggaccaagtt gctctgcgtc atcaaagaca 601 ccaaactgct gtgctataaa agttccaagg accagcagcc tcagatggaa ctgccactcc 661 aaggctgtaa cattacgtac atcccgaaag acagcaaaaa gaagaagcac gagctgaaga 721 ttactcagca gggcacggac ccgcttgttc tcgccgtcca gagcaaggaa caggccgagc 781 agtggctgaa ggtgatcaaa gaagcctaca gtggttgtag tggccccgtg gattcagagt 841 gtcctcctcc accaagctcc ccggtgcaca aggcagaact ggagaagaaa ctgtcttcag 901 agagacccag ctcagatggg gagggtgttg tggaaaatgg aattaccaca tgtaatggaa 961 aggagcaagt gaagaggaag aaaagttcca aatcagaggc caagggcact gtgtcgaaag 1021 tcactgggaa aaaaatcacc aagatcatca gtctgggaaa gaaaaagccg tccacagacg 1081 agcagacctc ctcagctgag gaagatgttc ccacctgcgg ctatctgaac gtgctctcca 1141 acagccgctg gcgagagcgc tggtgccgag tgaaagataa caagctcatt ttccacaagg 1201 acaggaccga cctgaagacc catattgtgt ctattccgct ccgtggctgc gaggtgatcc 1261 cgggtttgga ttgtaaacat cctctgacgt tccggctgct gcgcaacggc caggaggttg 1321 cagtattgga ggcatcttct tctgaagaca tgggcaggtg gattgggatt ttactcgcag 1381 agacgggatc gtccacagac ccggaggctc tgcactatga ctacattgat gtggagatgt 1441 ctgcaagtgt cattcagaca gccaaacaga ccttctgttt catgaacagg cgtgttatat 1501 ctgctaaccc atatctaggg ggcacctcca acggctatgc ccaccccagc gggacggcac 1561 ttcattatga cgatgtcccg tgcatcaacg gctcgctcaa gggtaaaaag ccccccgtgg 1621 cgtctaatgg ggtcacagga aaagggaaga ctctgagcag tcagccaaag aaagcggatc 1681 ccgcggctgt tgtgaaaagg acgggttcga atgctgccca gtacaagtat ggcaagaacc 1741 gggtagaagc agatgccaag cggctacaga ccaaagagga ggagctgctg aagaggaaag 1801 aggccctgcg gaataggctg gcccagctcc gcaaggaaag aaaagacctt cgagcggcta 1861 ttgaagtgaa cgccggcagg aagccgcagg cgatcctgga ggagaagctg aagcagctgg 1921 aggaggagtg ccggcagaag gaggcggagc gtgtcagcct ggagctggag ctgacggagg 1981 tcaaggagag cctgaagaaa gcgctggcgg gcggagtcac cctggggctg gccatcgagc 2041 ccaagtcagg gacatcgagt ccacagtctc cagtgttccg gcaccggacc ctggaaaact 2101 cgcccatctc cagctgtgac accagtgaca ccgagggccc cgtgccggtg aacagcgcgg 2161 ccgtcttgaa gaagagccag gctgccccgg gcagctcccc ctgccgaggg catgtgctgc 2221 ggaaggccaa ggaatgggaa atgaagaacg ggacctaccc aactttcttg tacaaagtgg 2281 ttggtaagcc tatccctaac cctctcctcg gtctcgattc tacgtagtaa tgagctagcg 2341 ctaaccggtg gcgcgttaag tcgacaatca acctctggat ta