Construct: ORF ccsbBroad304_08801
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001194.1_s304c1, BRDN0000406423
- Derived from:
- ccsbBroadEn_08801
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- LDAH (60526)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_08801
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 60526 | LDAH | lipid droplet associated hy... | NM_021925.4 | 99.8% | 99.6% | 960T>A |
| 2 | human | 60526 | LDAH | lipid droplet associated hy... | XM_011533014.2 | 98% | 97.8% | 154_171del;978T>A |
| 3 | human | 60526 | LDAH | lipid droplet associated hy... | NM_001282719.1 | 86.6% | 85.8% | (many diffs) |
| 4 | human | 60526 | LDAH | lipid droplet associated hy... | NM_001282720.2 | 85.1% | 84.9% | 153_154ins144;816T>A |
| 5 | human | 60526 | LDAH | lipid droplet associated hy... | NM_001282721.1 | 59.8% | 59.6% | 0_1ins390;570T>A |
| 6 | human | 60526 | LDAH | lipid droplet associated hy... | NM_001282722.1 | 59.8% | 59.6% | 0_1ins390;570T>A |
| 7 | human | 60526 | LDAH | lipid droplet associated hy... | NM_001282723.1 | 49.6% | 48.9% | (many diffs) |
| 8 | human | 60526 | LDAH | lipid droplet associated hy... | XR_939702.3 | 47.1% | (many diffs) | |
| 9 | human | 60526 | LDAH | lipid droplet associated hy... | XR_001738877.2 | 46.1% | 1_55del;1015T>A;1031_2110del | |
| 10 | human | 60526 | LDAH | lipid droplet associated hy... | XR_001738875.2 | 44.7% | (many diffs) | |
| 11 | human | 60526 | LDAH | lipid droplet associated hy... | XR_001738878.2 | 40.9% | (many diffs) | |
| 12 | human | 60526 | LDAH | lipid droplet associated hy... | NM_001282724.2 | 36.5% | 35% | (many diffs) |
| 13 | human | 60526 | LDAH | lipid droplet associated hy... | XR_001738876.2 | 35.6% | 1_55del;1015T>A;1031_2732del | |
| 14 | human | 60526 | LDAH | lipid droplet associated hy... | XR_001738879.2 | 35% | (many diffs) | |
| 15 | human | 60526 | LDAH | lipid droplet associated hy... | XR_939701.2 | 34.7% | (many diffs) | |
| 16 | human | 60526 | LDAH | lipid droplet associated hy... | XR_939700.2 | 29% | (many diffs) | |
| 17 | human | 60526 | LDAH | lipid droplet associated hy... | XR_001738874.1 | 28.3% | (many diffs) | |
| 18 | human | 60526 | LDAH | lipid droplet associated hy... | XR_939703.2 | 18.5% | (many diffs) | |
| 19 | human | 60526 | LDAH | lipid droplet associated hy... | NR_104233.2 | 16.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1041
- ORF length:
- 975
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgga ctcagaactc aaggaagaaa ttcctgtgca tgaggaattc attttgtgtg 121 gtggagccga aacccaggtt ctaaaatgtg ggccctggac agacctcttt catgatcaaa 181 gtgtcaaaag gcctaagctg cttattttca ttattcctgg taacccaggt ttttctgcct 241 tttatgtgcc atttgcaaag gctttatact ctttgacaaa cagacgcttt ccagtttgga 301 ctatcagtca tgctgggcat gcgttggctc ccaaagacaa gaagattctt acaacatcag 361 aggattcaaa cgctcaagaa attaaggaca tttatggact aaatggacaa atagagcaca 421 aactagcttt cctgagaact catgtgccaa aggacatgaa acttgtgctc attggccatt 481 caataggcag ctatttcaca cttcagatgc tgaagcgagt ccctgagctc ccggtaattc 541 gtgcctttct gctctttcca acaattgaac gaatgtctga gtcacccaat ggcagaattg 601 ccactccact tttgtgctgg tttcgatatg ttctctatgt tactggctac ttattattga 661 aaccgtgtcc tgagacaatc aagtccttgc taatcagaag gggccttcaa gtaatgaacc 721 tagagaatga attttcacca ttgaatatat tagaaccatt ctgccttgct aatgctgcct 781 accttggggg ccaagaaatg atggaggtgg tgaagagaga tgacgaaacc ataaaggagc 841 atttatgtaa gcttacattt tattatggta ctatagatcc ttggtgtcca aaagagtact 901 atgaagacat taagaaggat tttccagaag gagacattcg actctgtgag aaaaacatac 961 ctcatgcttt catcacccat tttaaccagg aaatggcaga catgattgct gactccctaa 1021 aggaagactt gtccaaaatg tacccaactt tcttgtacaa agtggttggt aagcctatcc 1081 ctaaccctct cctcggtctc gattctacgt agtaatgagc tagcgctaac cggtggcgcg 1141 ttaagtcgac aatcaacctc tggatta