Construct: ORF ccsbBroad304_08807
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000565.1_s304c1, BRDN0000405027
- Derived from:
- ccsbBroadEn_08807
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- BCAN (63827)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_08807
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 63827 | BCAN | brevican | NM_021948.5 | 99.9% | 99.8% | 1510G>A |
| 2 | human | 63827 | BCAN | brevican | XM_011509866.1 | 99.9% | 99.8% | 1510G>A |
| 3 | human | 63827 | BCAN | brevican | XM_017002047.1 | 95.2% | 95.1% | 1_135del;1645G>A |
| 4 | human | 63827 | BCAN | brevican | NM_198427.2 | 72.7% | 71.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2799
- ORF length:
- 2733
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggc ccagctgttc ctgcccctgc tggcagccct ggtcctggcc caggctcctg 121 cagctttagc agatgttctg gaaggagaca gctcagagga ccgcgctttt cgcgtgcgca 181 tcgcgggcga cgcgccactg cagggcgtgc tcggcggcgc cctcaccatc ccttgccacg 241 tccactacct gcggccaccg ccgagccgcc gggctgtgct gggctctccg cgggtcaagt 301 ggactttcct gtcccggggc cgggaggcag aggtgctggt ggcgcgggga gtgcgcgtca 361 aggtgaacga ggcctaccgg ttccgcgtgg cactgcctgc gtacccagcg tcgctcaccg 421 acgtctccct ggcgctgagc gagctgcgcc ccaacgactc aggtatctat cgctgtgagg 481 tccagcacgg catcgatgac agcagcgacg ctgtggaggt caaggtcaaa ggggtcgtct 541 ttctctaccg agagggctct gcccgctatg ctttctcctt ttctggggcc caggaggcct 601 gtgcccgcat tggagcccac atcgccaccc cggagcagct ctatgccgcc taccttgggg 661 gctatgagca atgtgatgct ggctggctgt cggatcagac cgtgaggtat cccatccaga 721 ccccacgaga ggcctgttac ggagacatgg atggcttccc cggggtccgg aactatggtg 781 tggtggaccc ggatgacctc tatgatgtgt actgttatgc tgaagaccta aatggagaac 841 tgttcctggg tgaccctcca gagaagctga cattggagga agcacgggcg tactgccagg 901 agcggggtgc agagattgcc accacgggcc aactgtatgc agcctgggat ggtggcctgg 961 accactgcag cccagggtgg ctagctgatg gcagtgtgcg ctaccccatc gtcacaccca 1021 gccagcgctg tggtgggggc ttgcctggtg tcaagactct cttcctcttc cccaaccaga 1081 ctggcttccc caataagcac agccgcttca acgtctactg cttccgagac tcggcccagc 1141 cttctgccat ccctgaggcc tccaacccag cctccaaccc agcctctgat ggactagagg 1201 ctatcgtcac agtgacagag accctggagg aactgcagct gcctcaggaa gccacagaga 1261 gtgaatcccg tggggccatc tactccatcc ccatcatgga ggacggagga ggtggaagct 1321 ccactccaga agacccagca gaggccccta ggacgctcct agaatttgaa acacaatcca 1381 tggtaccgcc cacggggttc tcagaagagg aaggtaaggc attggaggaa gaagagaaat 1441 atgaagatga agaagagaaa gaggaggaag aagaagagga ggaggtggag gatgaggctc 1501 tgtgggcatg gcccagcgag ctcagcagcc cgggccctga ggcctctctc cccactgagc 1561 cagcagccca ggagaagtca ctctcccagg cgccagcaag ggcagtcctg cagcctggtg 1621 catcaccact tcctgatgga gagtcagaag cttccaggcc tccaagggtc catggaccac 1681 ctactgagac tctgcccact cccagggaga ggaacctagc atccccatca ccttccactc 1741 tggttgaggc aagagaggtg ggggaggcaa ctggtggtcc tgagctatct ggggtccctc 1801 gaggagagag cgaggagaca ggaagctccg agggtgcccc ttccctgctt ccagccacac 1861 gggcccctga gggtaccagg gagctggagg ccccctctga agataattct ggaagaactg 1921 ccccagcagg gacctcagtg caggcccagc cagtgctgcc cactgacagc gccagccgag 1981 gtggagtggc cgtggtcccc gcatcaggtg actgtgtccc cagcccctgc cacaatggtg 2041 ggacatgctt ggaggaggag gaaggggtcc gctgcctatg tctgcctggc tatggggggg 2101 acctgtgcga tgttggcctc cgcttctgca accccggctg ggacgccttc cagggcgcct 2161 gctacaagca cttttccaca cgaaggagct gggaggaggc agagacccag tgccggatgt 2221 acggcgcgca tctggccagc atcagcacac ccgaggaaca ggacttcatc aacaaccggt 2281 accgggagta ccagtggatc ggactcaacg acaggaccat cgaaggcgac ttcttgtggt 2341 cggatggcgt ccccctgctc tatgagaact ggaaccctgg gcagcctgac agctacttcc 2401 tgtctggaga gaactgcgtg gtcatggtgt ggcatgatca gggacaatgg agtgacgtgc 2461 cctgcaacta ccacctgtcc tacacctgca agatggggct ggtgtcctgt gggccgccac 2521 cggagctgcc cctggctcaa gtgttcggcc gcccacggct gcgctatgag gtggacactg 2581 tgcttcgcta ccggtgccgg gaaggactgg cccagcgcaa tctgccgctg atccgatgcc 2641 aagagaacgg tcgttgggag gccccccaga tctcctgtgt gcccagaaga cctgcccgag 2701 ctctgcaccc agaggaggac ccagaaggac gtcaggggag gctactggga cgctggaagg 2761 cgctgttgat ccccccttcc agccccatgc caggtcccta cccaactttc ttgtacaaag 2821 tggttggtaa gcctatccct aaccctctcc tcggtctcga ttctacgtag taatgagcta 2881 gcgctaaccg gtggcgcgtt aagtcgacaa tcaacctctg gatta