Construct: ORF ccsbBroad304_08811
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014546.1_s304c1, BRDN0000404226
- Derived from:
- ccsbBroadEn_08811
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NSUN3 (63899)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_08811
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 63899 | NSUN3 | NOP2/Sun RNA methyltransfer... | NM_022072.5 | 99.9% | 99.7% | 884C>T |
| 2 | human | 63899 | NSUN3 | NOP2/Sun RNA methyltransfer... | XM_024453699.1 | 58.3% | 56.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1086
- ORF length:
- 1020
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgct gacccagctg aaagcaaaat cagaggggaa gcttgcaaaa cagatttgca 121 aagttgtgtt ggatcatttt gaaaaacagt attccaaaga actcggagat gcctggaata 181 cagtaaggga gatactaaca tctccatcat gctggcaata tgctgtcctg cttaaccgat 241 tcaattatcc ttttgaactg gaaaaggatt tacatttgaa gggctatcac acactctctc 301 agggatcttt acccaactat cctaaatcag tgaagtgtta ccttagcaga actccgggcc 361 gaatcccttc agaaagacac caaattggaa acctgaaaaa atattatctc ctaaatgctg 421 cttctcttct cccagtgttg gctctggaat taagggatgg ggagaaggtt ctggatctct 481 gtgctgctcc tggagggaaa tcaatagctc tgctgcagtg tgcttgtcca ggttatcttc 541 attgtaatga atatgatagt ctgagattga ggtggctaag gcagacgttg gaatctttca 601 tcccacagcc tttgataaat gtaattaaag tgtctgaatt ggatggcaga aaaatgggag 661 atgcccagcc tgaaatgttt gacaaggtgt tagtggatgc tccgtgttca aatgatcgaa 721 gctggttgtt ttcttctgac tctcagaagg catcctgtag gataagtcaa aggaggaatt 781 tgcctcttct acagatagag ctgttaaggt ctgcaattaa ggccttacgt cctggaggga 841 tacttgtata ctctacatgc acgctttcca aggcagaaaa tcaagatgtg atcagtgaaa 901 ttttaaactc ccacggtaac atcatgccta tggacattaa aggaatagta aggacttgct 961 cccacgactt cacatttgct cccactggcc aggaatgtgg gctcttagtg attccagata 1021 agggcaaagc ctggggccca atgtatgtag ccaaattgaa gaaatcatgg agcacaggaa 1081 aatggtgccc aactttcttg tacaaagtgg ttggtaagcc tatccctaac cctctcctcg 1141 gtctcgattc tacgtagtaa tgagctagcg ctaaccggtg gcgcgttaag tcgacaatca 1201 acctctggat ta