Construct: ORF ccsbBroad304_08834

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF010519.1_s304c1, BRDN0000405380
Derived from:
ccsbBroadEn_08834
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
NPFFR1 (64106)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_08834
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 64106 NPFFR1 neuropeptide FF receptor 1 NM_022146.5 99.9% 100% 597G>T
2 mouse 237362 Npffr1 neuropeptide FF receptor 1 NM_001177511.1 86% 86.5% (many diffs)
3 mouse 237362 Npffr1 neuropeptide FF receptor 1 XM_011243444.2 62.5% 63.1% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
1359
ORF length:
1290
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcaccat ggagggggag ccctcccagc ctcccaacag cagttggccc ctaagtcaga
121atgggactaa cactgaggcc accccggcta caaacctcac cttctcctcc tactatcagc
181acacctcccc tgtggcggcc atgttcattg tggcctatgc gctcatcttc ctgctctgca
241tggtgggcaa caccctggtc tgtttcatcg tgctcaagaa ccggcacatg catactgtca
301ccaacatgtt catcctcaac ctggctgtca gtgacctgct ggtgggcatc ttctgcatgc
361ccaccaccct tgtggacaac ctcatcactg ggtggccctt cgacaatgcc acatgcaaga
421tgagcggctt ggtgcagggc atgtctgtgt cggcttccgt tttcacactg gtggccattg
481ctgtggaaag gttccgctgc atcgtgcacc ctttccgcga gaagctgacc ctgcggaagg
541cgctcgtcac catcgccgtc atctgggccc tggcgctgct catcatgtgt ccctcggccg
601tcacgctgac cgtcacccgt gaggagcacc acttcatggt ggacgcccgc aaccgctcct
661accctctcta ctcctgctgg gaggcctggc ccgagaaggg catgcgcagg gtctacacca
721ctgtgctctt ctcgcacatc tacctggcgc cgctggcgct catcgtggtc atgtacgccc
781gcatcgcgcg caagctctgc caggccccgg gcccggcccc cgggggcgag gaggctgcgg
841acccgcgagc atcgcggcgc agagcgcgcg tggtgcacat gctggtcatg gtggcgctgt
901tcttcacgct gtcctggctg ccgctctggg cgctgctgct gctcatcgac tacgggcagc
961tcagcgcgcc gcagctgcac ctggtcaccg tctacgcctt ccccttcgcg cactggctgg
1021ccttcttcaa cagcagcgcc aaccccatca tctacggcta cttcaacgag aacttccgcc
1081gcggcttcca ggccgccttc cgcgcccgcc tctgcccgcg cccgtcgggg agccacaagg
1141aggcctactc cgagcggccc ggcgggcttc tgcacaggcg ggtcttcgtg gtggtgcggc
1201ccagcgactc cgggctgccc tctgagtcgg gccctagcag tggggccccc aggcccggcc
1261gcctcccgct gcggaatggg cgggtggctc accacggctt gcccagggaa gggcctggct
1321gctcccacct gcccctcacc attccagcct gggatatctt gccaacttcc ttgtacaaag
1381tggttggtaa gcctatccct aaccctctcc tcggtctcga ttctacgtag taatgagcta
1441gcgctaaccg gtggcgcgtt aagtcgacaa tcaacctctg gatta

Download FASTA (ORF) (Full)