Construct: ORF ccsbBroad304_08840
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015884.1_s304c1, BRDN0000401116
- Derived from:
- ccsbBroadEn_08840
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- KIF9 (64147)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_08840
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 64147 | KIF9 | kinesin family member 9 | NM_001134878.2 | 99.9% | 99.8% | 1912C>T |
| 2 | human | 64147 | KIF9 | kinesin family member 9 | NM_182902.3 | 99.9% | 99.8% | 1912C>T |
| 3 | human | 64147 | KIF9 | kinesin family member 9 | XM_011534004.2 | 99.9% | 99.8% | 1912C>T |
| 4 | human | 64147 | KIF9 | kinesin family member 9 | XM_017007029.1 | 99.9% | 99.8% | 1912C>T |
| 5 | human | 64147 | KIF9 | kinesin family member 9 | XM_011534003.2 | 98.2% | 98.1% | 1_42del;1954C>T |
| 6 | human | 64147 | KIF9 | kinesin family member 9 | XM_005265388.4 | 95.9% | 95.8% | 1_99del;2011C>T |
| 7 | human | 64147 | KIF9 | kinesin family member 9 | NM_022342.5 | 91.7% | 91.6% | 1512_1513ins195;1717C>T |
| 8 | human | 64147 | KIF9 | kinesin family member 9 | XM_011534005.2 | 90.4% | 90.3% | 364_365ins225;1687C>T |
| 9 | human | 64147 | KIF9 | kinesin family member 9 | XM_017007030.1 | 90.4% | 90.3% | 364_365ins225;1687C>T |
| 10 | human | 64147 | KIF9 | kinesin family member 9 | XM_011534006.2 | 88.1% | 84.5% | 0_1ins172;86_87ins107;1633C>T |
| 11 | human | 64147 | KIF9 | kinesin family member 9 | XM_005265389.4 | 88% | 87.9% | 1_99del;1611_1612ins195;1816C>T |
| 12 | human | 64147 | KIF9 | kinesin family member 9 | XM_006713291.3 | 86.8% | 86.7% | 1_99del;463_464ins225;1786C>T |
| 13 | human | 64147 | KIF9 | kinesin family member 9 | XR_940488.2 | 85.2% | (many diffs) | |
| 14 | human | 64147 | KIF9 | kinesin family member 9 | XM_017007031.1 | 82.2% | 82.1% | 364_365ins225;1287_1288ins195;1492C>T |
| 15 | human | 64147 | KIF9 | kinesin family member 9 | XR_002959567.1 | 78.2% | (many diffs) | |
| 16 | human | 64147 | KIF9 | kinesin family member 9 | XR_940489.2 | 68.7% | (many diffs) | |
| 17 | human | 64147 | KIF9 | kinesin family member 9 | XM_011534007.2 | 58.9% | 58.4% | (many diffs) |
| 18 | mouse | 16578 | Kif9 | kinesin family member 9 | NM_010628.3 | 86.9% | 88.8% | (many diffs) |
| 19 | mouse | 16578 | Kif9 | kinesin family member 9 | NM_001163569.1 | 84.3% | 85.4% | (many diffs) |
| 20 | mouse | 16578 | Kif9 | kinesin family member 9 | XM_006511945.3 | 84.3% | 85.4% | (many diffs) |
| 21 | mouse | 16578 | Kif9 | kinesin family member 9 | XM_006511943.3 | 82.8% | 84% | (many diffs) |
| 22 | mouse | 16578 | Kif9 | kinesin family member 9 | XM_006511944.3 | 82.8% | 84% | (many diffs) |
| 23 | mouse | 16578 | Kif9 | kinesin family member 9 | XM_006511942.3 | 81.9% | 83.6% | (many diffs) |
| 24 | mouse | 16578 | Kif9 | kinesin family member 9 | XM_006511941.3 | 79.5% | 80.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2436
- ORF length:
- 2370
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggg tactaggaaa aaagttcatg catttgtccg tgtcaaaccc accgatgact 121 ttgctcatga aatgatcaga tacggagatg acaaaagaag cattgatatt cacttaaaaa 181 aagacattcg gagaggagtt gtcaataacc aacagacaga ctggtcgttt aagttggatg 241 gagttcttca cgatgcctcc caggacttgg tttatgagac agttgcaaag gatgtggttt 301 ctcaggccct cgatggctat aatggcacca tcatgtgtta tgggcagacg ggagctggca 361 agacatacac catgatgggg gcaactgaga attacaagca ccgggggatc ctccctcgtg 421 ccctgcagca ggtttttagg atgatcgaag aacgccccac acatgccatc actgtgcgtg 481 tttcctactt ggaaatctat aatgagagcc tgtttgatct cctgtccact ctgccctatg 541 ttggaccctc agtcacacca atgaccatcg tggaaaaccc tcaaggagtc ttcattaagg 601 gcttgtcagt tcacctcaca agtcaggagg aggatgcatt cagcctcctt tttgagggtg 661 agaccaacag gattatagcc tcccacacta tgaacaaaaa ctcttccaga tcacactgca 721 ttttcaccat ctacttagag gcccattccc ggaccttatc agaggaaaag tacatcactt 781 ccaaaattaa cttggtggat ctggcaggct cagagaggct ggggaagtct gggtctgagg 841 gccaagtcct gaaggaagcc acctacatca acaaatcgct ctcattcctg gagcaggcca 901 tcattgccct tggggaccag aagcgggacc acatcccctt tcggcagtgc aagctcaccc 961 acgctctgaa ggactcgtta gggggaaact gcaatatggt cctcgtgaca aacatctatg 1021 gagaagctgc ccagttagaa gaaacgctat cttcactgag atttgccagc aggatgaagc 1081 tagtcaccac tgagcctgcc atcaatgaaa agtatgatgc tgagagaatg gtcaagaacc 1141 tggagaagga actagcacta ctcaagcagg agctggctat ccatgacagc ctgaccaacc 1201 gcacctttgt gacctatgac cccatggatg aaatccagat tgctgagatc aactcccagg 1261 tgcggaggta cctggagggg acactggacg agatcgacat aatcagcctt agacagatca 1321 aggaggtgtt caaccagttc cgggtggttc tgagccaaca ggaacaggaa gtggagtcca 1381 ctttgcgcag gaagtacacc ctcattgaca ggaatgactt tgcagccatt tctgctatcc 1441 agaaggcggg gcttgtggat gttgatggcc acctagtggg tgagcctgaa ggacaaaact 1501 ttggactcgg agtcgcccct ttctctacca aacctgggaa gaaagccaag tccaagaaga 1561 cattcaaaga gccactcagc tccttggcaa gaaaggaagg tgccagcagc cctgtgaatg 1621 ggaaggactt ggattacgtt tccacctcca agacccagct ggtcccatcc tccaaagatg 1681 gggatgtcaa agacatgctt tcgcgggacc gggaaacttc cagcattgag ccccttccct 1741 cagactcccc gaaggaggaa ttacgcccaa ttaggcccga caccccaccc tccaaaccag 1801 tggcctttga ggagtttaag aatgagcaag gtagtgagat caaccgaatt ttcaaagaaa 1861 acaaatccat cttgaatgaa cggaggaaaa gggccagcga gaccacacag cacatcaatg 1921 ccatcaagcg ggagattgat gtgaccaagg aggccctgaa tttccagaag tcactatggg 1981 agaagcaagg caagtacgaa aacaaggggc tgatgatcat cgatgaggaa gaattcctgc 2041 tgatcctcaa gctcaaagac ctcaagaagc agtaccgcag cgagtaccag gacctgcgtg 2101 acctcagggc tgagatccag tattgccagc acctagtgga tcagtgtcgc caccgcctgc 2161 tcatggaatt tgacatctgg tacaatgagt cctttgtcat ccctgaggac atgcagatgg 2221 cactgaagcc aggcggcagc atccggccag gcatggtccc tgtgaacagg attgtgtctc 2281 tgggagaaga tgaccaggac aaattcagcc agctgcagca gagggtgctt cctgagggcc 2341 ctgattccat ctccttctac aatgccaaag tcaagataga gcagaagcat aattacttga 2401 aaaccatgat gggcctccag caggcacata gaaaataccc aactttcttg tacaaagtgg 2461 ttggtaagcc tatccctaac cctctcctcg gtctcgattc tacgtagtaa tgagctagcg 2521 ctaaccggtg gcgcgttaag tcgacaatca acctctggat ta