Construct: ORF ccsbBroad304_08865

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF011484.1_s304c1, BRDN0000461999
Derived from:
ccsbBroadEn_08865
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
PARP12 (64761)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_08865
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 64761 PARP12 poly(ADP-ribose) polymerase... NM_022750.4 99.8% 100% 1014C>A;1656C>T;1896C>G
2 human 64761 PARP12 poly(ADP-ribose) polymerase... XM_011516491.2 76.2% 70.6% (many diffs)
3 human 64761 PARP12 poly(ADP-ribose) polymerase... XM_005250038.3 69.1% 68% (many diffs)
4 human 64761 PARP12 poly(ADP-ribose) polymerase... NR_130117.2 64.8% (many diffs)
5 human 64761 PARP12 poly(ADP-ribose) polymerase... XM_005250039.4 59.8% 56.6% 1014C>A;1180_1181ins142;1260_1261ins701
6 human 64761 PARP12 poly(ADP-ribose) polymerase... XM_005250040.4 58.6% 56.6% (many diffs)
7 human 64761 PARP12 poly(ADP-ribose) polymerase... XR_927514.2 48.5% (many diffs)
8 human 64761 PARP12 poly(ADP-ribose) polymerase... XR_001744855.1 48.1% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
2169
ORF length:
2103
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatggc ccaggccggc gtcgtcggtg aggtcaccca ggtgctgtgc gcggccgggg
121gcgccctgga gttgcccgag ctgcggcgcc gcttgcggat gggcttgagc gccgacgcgc
181tggagcggct gctgcggcag cgtgggcgct tcgtggtggc ggtgcgggcg ggcggcgcag
241ccgcggcccc ggagcgcgtg gtgctggccg cctcgccgct gcgcctgtgt cgcgcgcacc
301agggctccaa gccgggctgc gtggggctct gcgcgcagct ccacctctgc aggttcatgg
361tctacggcgc ctgcaagttc ctgagagccg ggaagaactg taggaatagt cacagcttga
421caaccgaaca caacctgagt gtgctgagaa ctcatggcgt tgaccacctg agctataatg
481agctatgcca actcttgttt cagaacgacc cctggctttt gccagaaatt tgccaacatt
541acaacaaagg agatggaccc cacggctctt gtgcctttca aaagcagtgc atcaagctcc
601atatctgcca gtatttttta cagggggaat gcaagtttgg cactagctgt aagagatccc
661atgatttctc taattctgag aatctggaaa aattggagaa gttgggtatg agctcagacc
721tggtgagcag gctgcctacc atttatagaa atgcacatga catcaagaat aagagctctg
781cccccagcag agtgcctcct ctttttgtcc cacaggggac ttctgaaaga aaagacagtt
841caggttctgt gtccccaaac actcttagcc aggaggaggg tgatcagatc tgtttgtacc
901atatccggaa aagttgtagc tttcaagata agtgccatag agttcatttc catttgccgt
961atcgatggca attcttggat agaggcaaat gggaggattt ggacaacatg gaacttattg
1021aagaggcata ttgcaatccc aaaatagaaa ggatcctgtg ctctgagtca gccagtacat
1081ttcactctca ttgtctgaac tttaacgcca tgacttacgg tgctacccag gctcgccgcc
1141tctccacggc ctcctctgtc accaaacctc cacacttcat cctcaccact gactggattt
1201ggtactggag tgatgagttt ggttcttggc aggaatatgg aagacagggc acggtgcacc
1261ctgtgaccac tgtcagcagt agcgacgtgg agaaggccta cctggcctac tgtacaccgg
1321ggtctgacgg ccaggcagcc accttgaagt tccaggccgg aaagcacaac tacgagttag
1381atttcaaagc cttcgttcag aaaaacctgg tctatggcac aactaaaaag gtttgccgca
1441gacccaaata cgtgtctccc caggatgtga cgaccatgca aacctgcaat accaagtttc
1501caggcccgaa gagcatccca gactattggg actcctctgc cctgccagac ccaggctttc
1561agaagatcac ccttagttct tcctcggaag agtatcagaa ggtctggaac ctctttaacc
1621gcacgctgcc tttctacttt gttcagaaga ttgagcgagt acagaacctg gccctctggg
1681aagtctacca gtggcaaaaa ggacagatgc agaagcagaa tggagggaag gccgtggacg
1741agcggcagct gttccacggc accagcgcca tttttgtgga cgccatctgc cagcagaact
1801ttgactggcg ggtctgtggt gttcatggca cttcctacgg caaggggagc tactttgccc
1861gagatgctgc atattcccac cactacagca aatccgacac gcagacccac acgatgttcc
1921tggcccgggt gctggtgggc gagttcgtca ggggcaatgc gtcctttgtc cgtccgccgg
1981ccaaggaggg ctggagcaac gccttctatg atagctgcgt gaacagtgtg tccgacccct
2041ccatctttgt gatctttgag aaacaccagg tctacccaga gtatgtcatc cagtacacca
2101cctcctccaa gccctcggtc acaccctcca tcctgctggc cttgggctcc ctgttcagca
2161gccgacagtg cccaactttc ttgtacaaag tggttggtaa gcctatccct aaccctctcc
2221tcggtctcga ttctacgtag taatgagcta gcgctaaccg gtggcgcgtt aagtcgacaa
2281tcaacctctg gatta

Download FASTA (ORF) (Full)