Construct: ORF ccsbBroad304_08872

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF009875.1_s304c1, BRDN0000462333
Derived from:
ccsbBroadEn_08872
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
EFCAB6 (64800)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_08872
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 64800 EFCAB6 EF-hand calcium binding dom... NM_198856.3 99.8% 99.7% (many diffs)
2 human 64800 EFCAB6 EF-hand calcium binding dom... XM_011530321.3 93.8% 93.7% (many diffs)
3 human 64800 EFCAB6 EF-hand calcium binding dom... XM_024452262.1 93.8% 93.7% (many diffs)
4 human 64800 EFCAB6 EF-hand calcium binding dom... XM_017028912.2 93.1% 92.4% (many diffs)
5 human 64800 EFCAB6 EF-hand calcium binding dom... XM_011530323.2 93% 92.5% (many diffs)
6 human 64800 EFCAB6 EF-hand calcium binding dom... XM_005261705.4 90.9% 90.8% (many diffs)
7 human 64800 EFCAB6 EF-hand calcium binding dom... XM_011530319.3 90.9% 90.8% (many diffs)
8 human 64800 EFCAB6 EF-hand calcium binding dom... NM_022785.4 89.7% 89.6% (many diffs)
9 human 64800 EFCAB6 EF-hand calcium binding dom... XM_017028911.2 88.2% 88% (many diffs)
10 human 64800 EFCAB6 EF-hand calcium binding dom... XM_011530325.2 87.2% 87% (many diffs)
11 human 64800 EFCAB6 EF-hand calcium binding dom... XM_011530318.3 85.6% 85.1% (many diffs)
12 human 64800 EFCAB6 EF-hand calcium binding dom... XM_011530320.3 85% 81.4% (many diffs)
13 human 64800 EFCAB6 EF-hand calcium binding dom... XM_005261704.2 84.8% 84.7% (many diffs)
14 human 64800 EFCAB6 EF-hand calcium binding dom... XM_011530317.3 84.8% 84.7% (many diffs)
15 human 64800 EFCAB6 EF-hand calcium binding dom... XM_011530316.1 83.5% 83.3% (many diffs)
16 human 64800 EFCAB6 EF-hand calcium binding dom... XM_017028910.2 83.5% 83.3% (many diffs)
17 human 64800 EFCAB6 EF-hand calcium binding dom... XM_011530327.2 81.9% 80.6% (many diffs)
18 human 64800 EFCAB6 EF-hand calcium binding dom... XM_011530326.3 71.4% 70.4% (many diffs)
19 human 64800 EFCAB6 EF-hand calcium binding dom... XR_937896.3 69.6% (many diffs)
20 human 64800 EFCAB6 EF-hand calcium binding dom... XR_937895.3 65.8% (many diffs)
21 human 64800 EFCAB6 EF-hand calcium binding dom... XM_024452263.1 55.5% 55.4% (many diffs)
22 human 64800 EFCAB6 EF-hand calcium binding dom... XM_011530328.3 52.8% 52.8% (many diffs)
23 human 64800 EFCAB6 EF-hand calcium binding dom... XM_011530330.3 43.9% 43.8% (many diffs)
24 human 64800 EFCAB6 EF-hand calcium binding dom... XR_001755274.2 38.1% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
4116
ORF length:
4047
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcaccat gaattgctgc cgcacattaa gagaacttga aatccaagtg ggagaaaagg
121ttttcaaaaa cattaagact gttatgaaag cctttgagct cattgatgtt aacaagactg
181gactggttcg accgcaggag ctaagaaggg ttctggagac cttctgtatg aagttaagag
241acgaggaata cgaaaagttt tcgaaacact acaacatcca caaggatact gcagtagatt
301acaacgtgtt tttgaagaat ctcagcataa ataacgactt gaaccttaga tattgtatgg
361gaaatcaaga ggtctcattg gagaatcaac aagccaaaaa ttccaaaaag gaacgtttgc
421taggttctgc atcatctgaa gatatctgga gaaactactc cttggatgaa attgagagga
481acttttgtct acaactttcg aagtcttatg aaaaggttga aaaggccctc agtgcagggg
541acccctgtaa aggtggctac gtgtctttta attatctaaa gattgtcctc gacacttttg
601tataccaaat accaagaaga atttttatcc agttaatgaa aagatttgga cttaaagcca
661ccactaaaat caattggaag caatttctaa catcatttca tgagcctcag gggttgcaag
721ttagcagtaa aggtcccctg acaaaaagaa atagcatcaa ctctagaaat gaatctcaca
781aggaaaacat catcacaaag ttatttagac acactgaaga tcactctgcg tcactgaaga
841aagcattact gataatcaac actaaacccg atggaccgat aacaagagaa gaatttcgat
901atattctaaa ttgcatggct gtaaaactaa gcgattcaga attcaaagaa ctaatgcaaa
961tgcttgaccc tggggacact ggagtggtca acaccagcat gtttattgat ctgattgaag
1021agaactgtag gatgagaaag acatctccct gtacagatgc taagacacct ttcctcctgg
1081cctgggattc agtggaagaa attgttcatg atacaattac taggaaccta caagcttttt
1141ataacatgct acgctcatat gaccttggag acacagggcg cattggccga aataatttca
1201agaaaatcat gcacgtcttc tgtccatttt taacgaatgc acatttcata aaactctgca
1261gtaagattca ggacattggt tcaggaagaa tcctttacaa gaaacttttg gcatgcatag
1321gaattgatgg cccacccact gtctctccag ttcttgttcc aaaggatcag ctgttaagtg
1381aacatttaca aaaagatgaa cagcagcagc cagatctttc tgagagaacc aagctcacgg
1441aggataaaac caccctgacc aagaagatga ccacagaaga agtgattgaa aaattcaaaa
1501agtgtataca gcagcaggac ccggcattca aaaaacgatt tcttgacttc agcaaggagc
1561ctaatggaaa aattaacgtg catgacttta agaaggtact ggaagacact gggatgccca
1621tggacgatga tcagtatgcc ctgctgacca ctaaaatagg cttcgagaag gaagggatga
1681gctatcttga ttttgcagca ggatttgaag atcctccaat gagagggccg gaaaccactc
1741cgccgcagcc tccaactcct tcaaaaagtt acgtgaacag ccactttatc accgcagaag
1801aatgcctgaa gcttttccct aggaggctga aggagtcatt ccgggacccc tactctgcct
1861tctttaaaac agatgctgac agggatggca taatcaacat gcatgacctt cacagactgc
1921tcctgcatct actgcttaat ctcaaagacg acgagtttga gcgcttcctt ggccttcttg
1981gcttgagact tagtgtcact ttaaattttc gggaatttca aaatttgtgt gagaagagac
2041catggagaac agatgaagcg cctcaaagac tcattagacc aaaacagaag gttgcggatt
2101cagaactagc ttgtgagcag gctcatcagt atcttgttac caaagcaaaa aacagatggt
2161cagacttgtc taagaatttt ctagaaaccg ataatgaggg caatggcatt cttcgacgcc
2221gggacataaa gaacgcactg tacggttttg atattcccct cacaccaaga gagtttgaaa
2281agctttgggc aagatacgac accgagggaa aagggcacat tacttaccag gaatttttac
2341agaaattggg tattaactat tcgcctgctg tccatcggcc ctgtgcagag gattatttca
2401acttcatggg tcattttaca aagccacagc agctacagga agagatgaag gagctgcagc
2461agagcacaga gaaggctgtg gcagccaggg ataagcttat ggaccgccat caagatatca
2521gcaaagcatt caccaaaact gatcaatcca aaaccaacta catatccata tgcaagatgc
2581aggaagtgct ggaagaatgt ggatgttctc ttaccgaagg ggagctgacc catctgctaa
2641acagttgggg agtcagccgg catgataatg ctatcaatta cctcgacttc ctgagagcag
2701tggagaacag caagtcaaca ggagctcagc ccaaggaaaa agaagagagc atgccaatca
2761attttgcaac gctgaatcca caggaggttg tgaggaagat ccaggaagta gttgagtcct
2821cccagctggc tttgtccacg gcattttctg cattgggtaa agaggataca ggatttgtaa
2881aggctacaga attcggacaa gttcttaagg atttctgtta caaactaacg gacaatcagt
2941atcattattt tttgaggaaa ctaagaactc atctaacccc ctatataaat tggaaatatt
3001ttctccagaa ctttagctgt ttccttgagg agacagctga tgagtgggct gagaaaatgc
3061ccaaaggccc gccgcctacc tctcccaagg ccacagccga cagagacatc ctggctcgcc
3121tccacaaagc agtgacttcc cattaccatg ccatcaccca ggagtttgag aattttgaca
3181ccatgaaaac gaacaccatc tccagagagg agtttagggc catttgtaat cgccgcgtcc
3241aaatcctgac ggacgaacag tttggcagac tctggaacga gatgccagtc aatgccaagg
3301ggaggctgaa atacccggac ttcctgagca ggttcagttc cgagacagca gccacaccaa
3361tggccactgg tgactcggcc gtggcccaga gagggagcag tgtccctgac gtctcggaag
3421gcaccagatc tgccctctca ttgcccactc aggagctgag accagggtca aagtcgcaga
3481gccacccctg tactccagcg agcaccaccg tgatcccggg cactccaccc ttgcagaact
3541gtgatcccat agagagcagg ctccggaaga gaatccaggg ctgctggcgc cagctcctga
3601aagaatgcaa ggagaaggac gtggccagac agggggacat caacgcctcc gatttcctgg
3661ctcttgtgga gaaattcaac ctggacataa gcaaagagga gtgtcagcag ctcattataa
3721aatacgactt aaagagcaac gggaaatttg catactgtga cttcattcag agctgtgtcc
3781tcctgctaaa agcaaaggaa agctcactga tgcacaggat gaagatccag aatgcacaca
3841agatgaaaga agccggcgcg gagacgccat ctttttactc tgctctgctg cgtattcagc
3901ccaaaattgt gcactgctgg aggccaatgc ggcgcacgtt caaaagctat gatgaggctg
3961gaacagggct gctaagcgtc gcagatttca ggacggtcct gagacagtac agcatcaacc
4021tctctgagga agagttcttc catattctgg agtattacga taagacgctg tcttcaaaaa
4081tctcctacaa cgacttcctc cgggcattcc tccagttgcc aactttcttg tacaaagtgg
4141ttggtaagcc tatccctaac cctctcctcg gtctcgattc tacgtagtaa tgagctagcg
4201ctaaccggtg gcgcgttaag tcgacaatca acctctggat ta

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