Construct: ORF ccsbBroad304_08883
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002110.1_s304c1, BRDN0000402676
- Derived from:
- ccsbBroadEn_08883
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MRPS6 (64968)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_08883
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 64968 | MRPS6 | mitochondrial ribosomal pro... | NM_032476.4 | 99.7% | 100% | 360A>G |
| 2 | mouse | 121022 | Mrps6 | mitochondrial ribosomal pro... | NM_080456.1 | 83.7% | 85.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 441
- ORF length:
- 375
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgcc ccgctacgag ctggctttaa tcctgaaagc catgcagcgg ccagagactg 121 ctgctacttt gaaacgtacg atagaggccc tgatggacag aggagcaata gtgagggact 181 tggaaaacct gggtgaacga gcgcttcctt ataggatctc tgcccacagt cagcagcaca 241 acagaggcgg gtatttcttg gtggattttt atgcacccac cgcagctgtt gaaagcatgg 301 tggagcactt gtctcgagat atagatgtga ttagagggaa tattgtcaaa caccctctga 361 cccaggaact aaaagaatgt gaagggattg tcccagtccc actcgcagaa aaattatatt 421 ccacgaagaa gaggaagaag tgcccaactt tcttgtacaa agtggttggt aagcctatcc 481 ctaaccctct cctcggtctc gattctacgt agtaatgagc tagcgctaac cggtggcgcg 541 ttaagtcgac aatcaacctc tggatta