Construct: ORF ccsbBroad304_08975
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001707.1_s304c1, BRDN0000406707
- Derived from:
- ccsbBroadEn_08975
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CPSF7 (79869)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_08975
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 79869 | CPSF7 | cleavage and polyadenylatio... | NM_001136040.3 | 99.9% | 100% | 1209C>T |
| 2 | human | 79869 | CPSF7 | cleavage and polyadenylatio... | XM_005274299.4 | 99.9% | 100% | 1209C>T |
| 3 | human | 79869 | CPSF7 | cleavage and polyadenylatio... | XM_011545258.2 | 99.9% | 100% | 1209C>T |
| 4 | human | 79869 | CPSF7 | cleavage and polyadenylatio... | NM_001142565.2 | 98% | 98% | 523_524ins27;1182C>T |
| 5 | human | 79869 | CPSF7 | cleavage and polyadenylatio... | XM_011545259.2 | 98% | 98% | 523_524ins27;1182C>T |
| 6 | human | 79869 | CPSF7 | cleavage and polyadenylatio... | NM_024811.3 | 91.5% | 91.6% | 1_129del;1338C>T |
| 7 | human | 79869 | CPSF7 | cleavage and polyadenylatio... | XM_005274298.4 | 89.8% | 89.8% | 1_129del;652_653ins27;1311C>T |
| 8 | human | 79869 | CPSF7 | cleavage and polyadenylatio... | XM_005274303.4 | 80.6% | 80.6% | 1_2delATins272;939C>T |
| 9 | human | 79869 | CPSF7 | cleavage and polyadenylatio... | XM_011545260.2 | 80.6% | 80.6% | 1_2delATins272;939C>T |
| 10 | human | 79869 | CPSF7 | cleavage and polyadenylatio... | XM_011545261.2 | 80.6% | 80.6% | 1_2delATins272;939C>T |
| 11 | human | 79869 | CPSF7 | cleavage and polyadenylatio... | XM_011545257.2 | 78.5% | 78.5% | 1_129del;834_835ins201;1137C>T |
| 12 | human | 79869 | CPSF7 | cleavage and polyadenylatio... | XM_017018345.1 | 76.7% | 76.8% | (many diffs) |
| 13 | human | 79869 | CPSF7 | cleavage and polyadenylatio... | XM_011545262.3 | 50.2% | 50.3% | 0_1ins702;507C>T |
| 14 | human | 79869 | CPSF7 | cleavage and polyadenylatio... | XM_011545263.2 | 50.2% | 50.3% | 0_1ins702;507C>T |
| 15 | mouse | 269061 | Cpsf7 | cleavage and polyadenylatio... | NM_172302.3 | 93.4% | 98.7% | (many diffs) |
| 16 | mouse | 269061 | Cpsf7 | cleavage and polyadenylatio... | XM_006527116.3 | 93.4% | 98.7% | (many diffs) |
| 17 | mouse | 269061 | Cpsf7 | cleavage and polyadenylatio... | NM_001164272.1 | 91.6% | 96.8% | (many diffs) |
| 18 | mouse | 269061 | Cpsf7 | cleavage and polyadenylatio... | XM_006527117.2 | 78.8% | 79.5% | (many diffs) |
| 19 | mouse | 269061 | Cpsf7 | cleavage and polyadenylatio... | XM_006527119.1 | 78.8% | 79.5% | (many diffs) |
| 20 | mouse | 269061 | Cpsf7 | cleavage and polyadenylatio... | XM_017318214.1 | 78.8% | 79.5% | (many diffs) |
| 21 | mouse | 269061 | Cpsf7 | cleavage and polyadenylatio... | XM_006527120.2 | 77% | 77.6% | (many diffs) |
| 22 | mouse | 269061 | Cpsf7 | cleavage and polyadenylatio... | XM_017318215.1 | 77% | 77.6% | (many diffs) |
| 23 | mouse | 269061 | Cpsf7 | cleavage and polyadenylatio... | XM_006527123.2 | 47.4% | 49.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1479
- ORF length:
- 1413
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgtc agaaggagtg gacttgattg atatatatgc tgacgaggag ttcaaccagg 121 acccagagtt caacaataca gatcagattg acctgtatga tgatgtgctg acagccacct 181 cacagccctc agatgacaga agcagcagca ctgaaccacc tcctcctgtt cgccaggagc 241 catctcccaa gcccaacaac aagacccctg caattctgta tacctacagt ggcctgcgta 301 atagacgagc tgccgtttat gtgggcagct tctcctggtg gaccacagac cagcagctga 361 tccaggttat tcgctctata ggagtctatg atgtggtgga gttgaaattt gcagagaatc 421 gagcaaatgg ccagtccaaa gggtatgctg aggtggtggt agcctctgaa aactctgtcc 481 acaaattgtt ggaactccta ccagggaaag ttcttaatgg agaaaaagtg gacgtgaggc 541 cggccacccg gcagaacctg tcacagtttg aggcacaggc tcggaaacgt gagtgtgtcc 601 gagtcccaag agggggaata cctccacggg cccattcccg agattctagt gattctgctg 661 atggacgggc cacaccctct gagaaccttg taccctcatc tgctcgtgtg gataagcccc 721 ccagtgtgct gccctacttc aatcgtcctc cttcggccct tcccctgatg ggtctgcccc 781 caccaccaat tccaccccca ccacctctct cctcaagctt tggggtccct cctcctcctc 841 ctggtatcca ctaccagcat ctcatgcccc cacctcctcg attacctcct catcttgctg 901 tacctccccc tggggccatc ccacctgccc ttcacctcaa tccagccttc ttccccccac 961 caaacgctac agtggggcct ccaccagata cttacatgaa ggcctctgcc ccctataacc 1021 accatggcag ccgagattcg ggccctccac cctctacagt gagtgaagcc gaatttgaag 1081 atatcatgaa gcgaaacaga gcaatttcca gcagtgccat ttccaaagca gtatctggag 1141 ccagtgcagg ggattacagt gacgcaattg agacgctgct cacagccatt gcggttatca 1201 aacagtcccg ggttgccaat gatgagcgtt gccgtgtcct catctcctct cttaaggact 1261 gtcttcatgg cattgaagcc aagtcctaca gtgtgggtgc cagtgggagc tcttccagga 1321 aaagacatcg ctcccgggaa aggtcaccta gccggtcccg ggagagcagc aggaggcacc 1381 gggatctgct tcataatgaa gatcggcatg atgattattt ccaagaaagg aaccgggagc 1441 atgagagaca ccgggataga gaacgggacc ggcaccactg cccaactttc ttgtacaaag 1501 tggttggtaa gcctatccct aaccctctcc tcggtctcga ttctacgtag taatgagcta 1561 gcgctaaccg gtggcgcgtt aagtcgacaa tcaacctctg gatta