Construct: ORF ccsbBroad304_08995
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000931.1_s304c1, BRDN0000400829
- Derived from:
- ccsbBroadEn_08995
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- WDR76 (79968)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_08995
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 79968 | WDR76 | WD repeat domain 76 | NM_024908.4 | 99.9% | 99.8% | 457T>G |
| 2 | human | 79968 | WDR76 | WD repeat domain 76 | NM_001167941.2 | 89.7% | 89.6% | 0_1ins192;265T>G |
| 3 | human | 79968 | WDR76 | WD repeat domain 76 | XM_005254680.3 | 89.7% | 89.6% | 0_1ins192;265T>G |
| 4 | human | 79968 | WDR76 | WD repeat domain 76 | XR_001751398.2 | 49% | 1_40del;497T>G;1919_3829del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1944
- ORF length:
- 1878
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgtc caggtcgggc gcggcggctg agaaggcgga ctccagacag cgaccccaga 121 tgaaggtaaa tgaatataaa gaaaatcaaa acatcgctta tgtgtctctg agaccagcac 181 agactacagt tttaataaaa acagctaagg tctatcttgc ccccttttca ctcagtaatt 241 accagctaga ccagcttatg tgccccaaat ccctatcaga aaagaattct aacaatgaag 301 tggcgtgtaa gaagactaaa ataaagaaaa cttgcagaag gattatacct ccaaagatga 361 aaaacacatc ttccaaggca gaatccacgc tgcaaaattc atcctcagct gttcatactg 421 aaagtaacaa gctacaaccc aagagaacgg cagatgcgat gaatctcagt gttgatgtgg 481 aaagtagtca ggatggagac agtgatgaag ataccacacc agccctggat ttttcgggat 541 tgtcacccta cgaaaggaag agactgaaga acatatcaga aaacgcagac ttttttgctt 601 ctcttcagtt gtctgagtct gctgcaagac tccgtgaaat gatagagaag agacagcctc 661 ctaaatccaa aagaaagaag cctaagagag aaaatgggat tggatgtaga aggtcaatgc 721 gattactaaa agttgatcct tcgggagttt cattaccagc agctccaaca ccgccgacat 781 tagtagcaga tgaaactcct ttgttacctc ctgggccttt agaaatgact tctgaaaatc 841 aagaagacaa caatgaacga tttaaaggat ttctgcacac atgggcagga atgagcaagc 901 caagtagtaa gaacactgag aagggattat ctagcattaa aagctacaaa gccaatttaa 961 atggcatggt cattagtgaa gataccgttt acaaagttac cacaggccca atattctcta 1021 tggctctcca tccatcagaa actagaactt tggtagcagt tggggccaaa tttgggcaag 1081 ttggactttg tgatttgacc cagcaaccta aagaagatgg agtttatgtt tttcatcccc 1141 atagtcagcc agttagctgt ctttacttct cacccgccaa tccggcccac atactgtcac 1201 tgagctatga tggcacgtta cgctgtgggg atttttccag ggctattttt gaagaggtgt 1261 atagaaatga aagaagtagc ttttcctcct tcgacttctt ggcagaagat gcctccactt 1321 taatagtagg acactgggat ggaaatatgt cactggtgga tagacggaca cctggaactt 1381 cttatgagaa acttaccagt tcttctatgg gaaaaataag aactgttcat gtccacccag 1441 tgcatagaca gtattttatc actgccggat tgagggatac tcatatttat gatgcaaggc 1501 gattgaattc caggagaagt cagcctttga tttctttgac tgaacataca aagagcattg 1561 cttccgccta tttttcacct cttactggta acagagtggt gaccacatgt gctgattgta 1621 atctgagaat ttttgacagc agctgtatat cttctaagat tccgctcctc accaccatca 1681 ggcacaacac tttcactggg cgatggctga ccaggttcca agccatgtgg gatcctaaac 1741 aagaagactg tgtcatagtt ggcagcatgg cccatccacg acgggtagaa atcttccatg 1801 agacaggaaa gagggtgcat tcgtttggtg gagaatacct tgtctctgtg tgttccatca 1861 atgccatgca cccaactcgg tatattttgg ctggaggtaa ttccagcggg aagatacatg 1921 tttttatgaa tgaaaaaagc tgctgcccaa ctttcttgta caaagtggtt ggtaagccta 1981 tccctaaccc tctcctcggt ctcgattcta cgtagtaatg agctagcgct aaccggtggc 2041 gcgttaagtc gacaatcaac ctctggatta